Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells

To fully realize the potential of human pluripotent stem cells (hPSCs) for both therapeutic and research purposes, it is critical to follow an efficient and reliable in vitro differentiation method that is based on optimal physical, chemical and developmental cues. This highly reproducible protocol...

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Veröffentlicht in:	Nature protocols 2022-03, Vol.17 (3), p.890-909
Hauptverfasser:	Kim, Jisun, Jeon, Jeha, Song, Bin, Lee, Nayeon, Ko, Sanghyeok, Cha, Young, Leblanc, Pierre, Seo, Hyemyung, Kim, Kwang-Soo
Format:	Artikel
Sprache:	eng
Schlagworte:	6-Hydroxydopamine 631/1647/1407/651 631/532/2182 692/617/375/1718 Analytical Chemistry Animals Autografts Biological Techniques Biomedical and Life Sciences Cell culture Cell Differentiation Computational Biology/Bioinformatics Confined spaces Culture Differentiation Dopamine Dopamine - metabolism Dopamine receptors Dopaminergic Neurons Embryo cells Gene expression Humans In vitro methods and tests Induced Pluripotent Stem Cells Life Sciences Mesencephalon Microarrays Movement disorders Neostriatum Neurodegenerative diseases Neurons Optimization Organic Chemistry Parkinson's disease Pluripotency Pluripotent Stem Cells Progenitor cells Protocol Quality control Rats Stem cell transplantation Stem cells
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