Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability
The sodA gene encoding the Corynebacterium melassecola manganese-cofactored superoxide dismutase (SOD) has been cloned in Escherichia coli and sequenced. The gene is transcribed monocistronically; the predicted polypeptide is 200 amino acids long and associates in a homotetrameric, manganese-depende...
Gespeichert in:
| Veröffentlicht in: | Journal of bacteriology 2001-02, Vol.183 (4), p.1284-1295 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1295 |
|---|---|
| container_issue | 4 |
| container_start_page | 1284 |
| container_title | Journal of bacteriology |
| container_volume | 183 |
| creator | Merkamm, M Guyonvarch, A |
| description | The sodA gene encoding the Corynebacterium melassecola manganese-cofactored superoxide dismutase (SOD) has been cloned in Escherichia coli and sequenced. The gene is transcribed monocistronically; the predicted polypeptide is 200 amino acids long and associates in a homotetrameric, manganese-dependent form, able to complement an SOD-deficient E. coli mutant. A second open reading frame, coding for a putative 217-amino-acid protein with high homology to peptide methionine sulfoxide reductases from various origins, has been identified immediately upstream of sodA in the opposite transcription orientation. The sodA gene was inactivated by insertion of an integrative vector carrying a kanamycin resistance gene. The growth rate of the SOD-deficient integrant was only slightly affected in BHI rich medium as well as in BMCG chemically defined medium, but was strongly affected by the presence of the redox-cycling agent paraquat. The SOD deficiency had, on the other hand, a deleterious effect on viability as soon as the culture entered the stationary phase of growth in BHI medium. Surprisingly, SOD deficiency was able to rescue the dramatic loss of viability observed for the wild-type strain in BMCG synthetic medium when glucose was not the limiting growth factor. |
| doi_str_mv | 10.1128/JB.2001.183.4.1284-1295.2001 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_95002</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17827800</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-5763d4e793d67ef997cd921a162c7523edc15b0ae4c119db241223dbf5828ea63</originalsourceid><addsrcrecordid>eNpdkU9v1DAQxS0EokvhKyALIW4JHttZxxKXdsW_qhIXOFtOPNm6cuzFTqrutycpKwqcRnrznjXPP0LeAqsBePv-6rLmjEENrahlvSiyAq6bB_EJ2QDTbdU0gj0lG8Y4VBq0OCMvSrldDFI2_Dk5A4BGaQkbctiFFH3c0zTQ6QZpSe6C7jEiHXIa6S7lY8TO9hNmP490xGBLwT4FS210NKeAa7TMB8zp3jukzpdxnmxB6iPtMYQ52EzvvO188NPxJXk22FDw1Wmekx-fPn7ffamuv33-uru4rnrZyqlq1FY4iUoLt1U4aK16pzlY2PJeNVyg66HpmEXZA2jXcQmcC9cNTctbtFtxTj78fvcwd-PixjhlG8wh-9Hmo0nWm3830d-Yfbozulm-bYm_O8Vz-jljmczoy9rGRkxzMaBarlrGFuOb_4y3ac5xqWY4V2yrtBKPx_Q5lZJx-HMHMLNSNVeXZgVoFqpGmpWqWak-iEv89d9dHsMnjOIX8F2g1w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>227067973</pqid></control><display><type>article</type><title>Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Merkamm, M ; Guyonvarch, A</creator><creatorcontrib>Merkamm, M ; Guyonvarch, A</creatorcontrib><description>The sodA gene encoding the Corynebacterium melassecola manganese-cofactored superoxide dismutase (SOD) has been cloned in Escherichia coli and sequenced. The gene is transcribed monocistronically; the predicted polypeptide is 200 amino acids long and associates in a homotetrameric, manganese-dependent form, able to complement an SOD-deficient E. coli mutant. A second open reading frame, coding for a putative 217-amino-acid protein with high homology to peptide methionine sulfoxide reductases from various origins, has been identified immediately upstream of sodA in the opposite transcription orientation. The sodA gene was inactivated by insertion of an integrative vector carrying a kanamycin resistance gene. The growth rate of the SOD-deficient integrant was only slightly affected in BHI rich medium as well as in BMCG chemically defined medium, but was strongly affected by the presence of the redox-cycling agent paraquat. The SOD deficiency had, on the other hand, a deleterious effect on viability as soon as the culture entered the stationary phase of growth in BHI medium. Surprisingly, SOD deficiency was able to rescue the dramatic loss of viability observed for the wild-type strain in BMCG synthetic medium when glucose was not the limiting growth factor.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JB.2001.183.4.1284-1295.2001</identifier><identifier>PMID: 11157941</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Amino Acid Sequence ; Anatomy & physiology ; Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; Cells ; Chromosomes, Bacterial ; Cloning, Molecular ; Corynebacterium - enzymology ; Corynebacterium - genetics ; Corynebacterium melassecola ; Genes ; Genes, Bacterial ; Manganese ; Membrane Transport Proteins ; Metabolism ; Methionine Sulfoxide Reductases ; Molecular Sequence Data ; Mutagenesis, Insertional ; Open Reading Frames ; Oxidative Stress - genetics ; Oxidoreductases - genetics ; paraquat ; Physiology and Metabolism ; Recombinant Proteins - biosynthesis ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; sodA gene ; Superoxide Dismutase - biosynthesis ; Superoxide Dismutase - genetics ; Superoxides - metabolism ; viability</subject><ispartof>Journal of bacteriology, 2001-02, Vol.183 (4), p.1284-1295</ispartof><rights>Copyright American Society for Microbiology Feb 2001</rights><rights>Copyright © 2001, American Society for Microbiology 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-5763d4e793d67ef997cd921a162c7523edc15b0ae4c119db241223dbf5828ea63</citedby><cites>FETCH-LOGICAL-c484t-5763d4e793d67ef997cd921a162c7523edc15b0ae4c119db241223dbf5828ea63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC95002/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC95002/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11157941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Merkamm, M</creatorcontrib><creatorcontrib>Guyonvarch, A</creatorcontrib><title>Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability</title><title>Journal of bacteriology</title><addtitle>J Bacteriol</addtitle><description>The sodA gene encoding the Corynebacterium melassecola manganese-cofactored superoxide dismutase (SOD) has been cloned in Escherichia coli and sequenced. The gene is transcribed monocistronically; the predicted polypeptide is 200 amino acids long and associates in a homotetrameric, manganese-dependent form, able to complement an SOD-deficient E. coli mutant. A second open reading frame, coding for a putative 217-amino-acid protein with high homology to peptide methionine sulfoxide reductases from various origins, has been identified immediately upstream of sodA in the opposite transcription orientation. The sodA gene was inactivated by insertion of an integrative vector carrying a kanamycin resistance gene. The growth rate of the SOD-deficient integrant was only slightly affected in BHI rich medium as well as in BMCG chemically defined medium, but was strongly affected by the presence of the redox-cycling agent paraquat. The SOD deficiency had, on the other hand, a deleterious effect on viability as soon as the culture entered the stationary phase of growth in BHI medium. Surprisingly, SOD deficiency was able to rescue the dramatic loss of viability observed for the wild-type strain in BMCG synthetic medium when glucose was not the limiting growth factor.</description><subject>Amino Acid Sequence</subject><subject>Anatomy & physiology</subject><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Cells</subject><subject>Chromosomes, Bacterial</subject><subject>Cloning, Molecular</subject><subject>Corynebacterium - enzymology</subject><subject>Corynebacterium - genetics</subject><subject>Corynebacterium melassecola</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Manganese</subject><subject>Membrane Transport Proteins</subject><subject>Metabolism</subject><subject>Methionine Sulfoxide Reductases</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>Open Reading Frames</subject><subject>Oxidative Stress - genetics</subject><subject>Oxidoreductases - genetics</subject><subject>paraquat</subject><subject>Physiology and Metabolism</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>sodA gene</subject><subject>Superoxide Dismutase - biosynthesis</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxides - metabolism</subject><subject>viability</subject><issn>0021-9193</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU9v1DAQxS0EokvhKyALIW4JHttZxxKXdsW_qhIXOFtOPNm6cuzFTqrutycpKwqcRnrznjXPP0LeAqsBePv-6rLmjEENrahlvSiyAq6bB_EJ2QDTbdU0gj0lG8Y4VBq0OCMvSrldDFI2_Dk5A4BGaQkbctiFFH3c0zTQ6QZpSe6C7jEiHXIa6S7lY8TO9hNmP490xGBLwT4FS210NKeAa7TMB8zp3jukzpdxnmxB6iPtMYQ52EzvvO188NPxJXk22FDw1Wmekx-fPn7ffamuv33-uru4rnrZyqlq1FY4iUoLt1U4aK16pzlY2PJeNVyg66HpmEXZA2jXcQmcC9cNTctbtFtxTj78fvcwd-PixjhlG8wh-9Hmo0nWm3830d-Yfbozulm-bYm_O8Vz-jljmczoy9rGRkxzMaBarlrGFuOb_4y3ac5xqWY4V2yrtBKPx_Q5lZJx-HMHMLNSNVeXZgVoFqpGmpWqWak-iEv89d9dHsMnjOIX8F2g1w</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Merkamm, M</creator><creator>Guyonvarch, A</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20010201</creationdate><title>Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability</title><author>Merkamm, M ; Guyonvarch, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-5763d4e793d67ef997cd921a162c7523edc15b0ae4c119db241223dbf5828ea63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Anatomy & physiology</topic><topic>Bacteria</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Cells</topic><topic>Chromosomes, Bacterial</topic><topic>Cloning, Molecular</topic><topic>Corynebacterium - enzymology</topic><topic>Corynebacterium - genetics</topic><topic>Corynebacterium melassecola</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Manganese</topic><topic>Membrane Transport Proteins</topic><topic>Metabolism</topic><topic>Methionine Sulfoxide Reductases</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>Open Reading Frames</topic><topic>Oxidative Stress - genetics</topic><topic>Oxidoreductases - genetics</topic><topic>paraquat</topic><topic>Physiology and Metabolism</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>sodA gene</topic><topic>Superoxide Dismutase - biosynthesis</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxides - metabolism</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Merkamm, M</creatorcontrib><creatorcontrib>Guyonvarch, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Merkamm, M</au><au>Guyonvarch, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability</atitle><jtitle>Journal of bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>183</volume><issue>4</issue><spage>1284</spage><epage>1295</epage><pages>1284-1295</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><coden>JOBAAY</coden><abstract>The sodA gene encoding the Corynebacterium melassecola manganese-cofactored superoxide dismutase (SOD) has been cloned in Escherichia coli and sequenced. The gene is transcribed monocistronically; the predicted polypeptide is 200 amino acids long and associates in a homotetrameric, manganese-dependent form, able to complement an SOD-deficient E. coli mutant. A second open reading frame, coding for a putative 217-amino-acid protein with high homology to peptide methionine sulfoxide reductases from various origins, has been identified immediately upstream of sodA in the opposite transcription orientation. The sodA gene was inactivated by insertion of an integrative vector carrying a kanamycin resistance gene. The growth rate of the SOD-deficient integrant was only slightly affected in BHI rich medium as well as in BMCG chemically defined medium, but was strongly affected by the presence of the redox-cycling agent paraquat. The SOD deficiency had, on the other hand, a deleterious effect on viability as soon as the culture entered the stationary phase of growth in BHI medium. Surprisingly, SOD deficiency was able to rescue the dramatic loss of viability observed for the wild-type strain in BMCG synthetic medium when glucose was not the limiting growth factor.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>11157941</pmid><doi>10.1128/JB.2001.183.4.1284-1295.2001</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9193 |
| ispartof | Journal of bacteriology, 2001-02, Vol.183 (4), p.1284-1295 |
| issn | 0021-9193 1098-5530 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_95002 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amino Acid Sequence Anatomy & physiology Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacteriology Base Sequence Cells Chromosomes, Bacterial Cloning, Molecular Corynebacterium - enzymology Corynebacterium - genetics Corynebacterium melassecola Genes Genes, Bacterial Manganese Membrane Transport Proteins Metabolism Methionine Sulfoxide Reductases Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames Oxidative Stress - genetics Oxidoreductases - genetics paraquat Physiology and Metabolism Recombinant Proteins - biosynthesis Sequence Analysis, DNA Sequence Homology, Amino Acid sodA gene Superoxide Dismutase - biosynthesis Superoxide Dismutase - genetics Superoxides - metabolism viability |
| title | Cloning of the sodA gene from Corynebacterium melassecola and role of superoxide dismutase in cellular viability |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T07%3A17%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20the%20sodA%20gene%20from%20Corynebacterium%20melassecola%20and%20role%20of%20superoxide%20dismutase%20in%20cellular%20viability&rft.jtitle=Journal%20of%20bacteriology&rft.au=Merkamm,%20M&rft.date=2001-02-01&rft.volume=183&rft.issue=4&rft.spage=1284&rft.epage=1295&rft.pages=1284-1295&rft.issn=0021-9193&rft.eissn=1098-5530&rft.coden=JOBAAY&rft_id=info:doi/10.1128/JB.2001.183.4.1284-1295.2001&rft_dat=%3Cproquest_pubme%3E17827800%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=227067973&rft_id=info:pmid/11157941&rfr_iscdi=true |