Simple Fluorescence Sensing Approach for Selective Detection of Fe3+ Ions: Live-Cell Imaging and Logic Gate Functioning

Simple Fluorescence Sensing Approach for Selective Detection of Fe3+ Ions: Live-Cell Imaging and Logic Gate Functioning

A pyrene-based fluorescent chemosensor APSB [N-(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe3+ in the presence of other metal ions. Apart fro...

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Veröffentlicht in:ACS omega 2022-09, Vol.7 (37), p.33248-33257
Hauptverfasser: Rasin, Puthiyavalappil, Manakkadan, Vipin, Vadakkedathu Palakkeezhillam, Vishnunarayanan Namboothiri, Haribabu, Jebiti, Echeverria, Cesar, Sreekanth, Anandaram
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container_end_page 33257
container_issue 37
container_start_page 33248
container_title ACS omega
container_volume 7
creator Rasin, Puthiyavalappil
Manakkadan, Vipin
Vadakkedathu Palakkeezhillam, Vishnunarayanan Namboothiri
Haribabu, Jebiti
Echeverria, Cesar
Sreekanth, Anandaram
description A pyrene-based fluorescent chemosensor APSB [N-(pyrene-1-ylmethylene) anthracen-2-amine] was designed and developed by a simple condensation reaction between pyrene carboxaldehyde and 2-aminoanthracene. The APSB fluorescent sensor selectively binds Fe3+ in the presence of other metal ions. Apart from this, APSB shows high selectivity and sensitivity toward Fe3+ ion detection. The detection limit for APSB was 1.95 nM, and the binding constant (K b) was obtained as 8.20 × 105 M–1 in DMSO/water (95/5, v/v) medium. The fluorescence quantum yields for APSB and APSB–Fe3+ were calculated as 0.035 and 0.573, respectively. The function of this fluorescent sensor APSB can be explained through the photo-induced electron transfer mechanism which was further proved by density functional theory studies. Finally, a live-cell image study of APSB in HeLa cells was also carried out to investigate the cell permeability of APSB and its efficiency for selective detection of Fe3+ in living cells.
doi_str_mv 10.1021/acsomega.2c03718
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title Simple Fluorescence Sensing Approach for Selective Detection of Fe3+ Ions: Live-Cell Imaging and Logic Gate Functioning
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