In vivo tracking of individual stem cells labeled with nanowire lasers using multimodality imaging

Emerging cell-based regenerative medicine and stem cell therapies have drawn wide attention in medical research and clinical practice to treat tissue damage and numerous incurable diseases. In vivo observation of the distribution, migration, and development of the transplanted cells is important for...

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Veröffentlicht in:Biomedical optics express 2022-09, Vol.13 (9), p.4706-4717
Hauptverfasser: Li, Xuzhou, Zhang, Wei, Li, Yanxiu, Wu, Xiaoqin, Wang, Mingyang, Tan, Xiaotian, Paulus, Yannis M., Fan, Xudong, Wang, Xueding
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container_end_page 4717
container_issue 9
container_start_page 4706
container_title Biomedical optics express
container_volume 13
creator Li, Xuzhou
Zhang, Wei
Li, Yanxiu
Wu, Xiaoqin
Wang, Mingyang
Tan, Xiaotian
Paulus, Yannis M.
Fan, Xudong
Wang, Xueding
description Emerging cell-based regenerative medicine and stem cell therapies have drawn wide attention in medical research and clinical practice to treat tissue damage and numerous incurable diseases. In vivo observation of the distribution, migration, and development of the transplanted cells is important for both understanding the mechanism and evaluating the treatment efficacy and safety. However, tracking the 3D migration trajectories for individual therapeutic cells in clinically relevant pathological environments remains technically challenging. Using a laser photocoagulation model in living rabbit eyes, this study demonstrates a multimodality imaging technology integrating optical coherence tomography (OCT), fluorescence microscopy (FM), and lasing emission for in vivo longitudinal tracking of the 3D migration trajectories of individual human retinal pigment epithelium cells (ARPE-19) labeled with CdS nanowires. With unique lasing spectra generated from the subtle microcavity differences, the surface-modified nanowires perform as distinct spectral identifiers for labeling individual ARPE-19 cells. Meanwhile, with strong optical scattering and natural fluorescence emission, CdS nanowires also served as OCT and FM contrast agents to indicate the spatial locations of the transplanted ARPE-19 cells. A longitudinal study of tracking individual ARPE-19 cells in rabbit eyes over a duration of 28 days was accomplished. This method could potentially promote an understanding of the pharmacodynamics and pharmacokinetics of implanted cells in the development of cell-based therapies.
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title In vivo tracking of individual stem cells labeled with nanowire lasers using multimodality imaging
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