A single-transformation gene function test in diploid Candida albicans

The fungal pathogen Candida albicans is naturally diploid, and current gene disruption strategies require two successive transformations. We describe here a genetic construct (UAU1) for which two copies may be selected. Insertion of UAU1 into one genomic site, after a single transformation, allows s...

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Veröffentlicht in:	Journal of bacteriology 2000-10, Vol.182 (20), p.5730-5736
Hauptverfasser:	Enloe, B, Diamond, A, Mitchell, A P
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Base Sequence Candida albicans Candida albicans - genetics Candida albicans - growth & development CDC25 gene CDC28 gene Diploidy DNA Primers Eukaryotic Cells Fungi Gene Deletion Genes Genetics Genome, Fungal Homozygote Molecular Sequence Data Mutagenesis, Insertional Plasmids Polymerase Chain Reaction Recombination, Genetic SNF1 gene Transformation, Genetic UAU1 gene
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