ssgA is essential for sporulation of Streptomyces coelicolor A3(2) and affects hyphal development by stimulating septum formation
The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was...
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| Veröffentlicht in: | Journal of bacteriology 2000-10, Vol.182 (20), p.5653-5662 |
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| creator | van Wezel, G P van der Meulen, J Kawamoto, S Luiten, R G Koerten, H K Kraal, B |
| description | The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division. |
| doi_str_mv | 10.1128/JB.182.20.5653-5662.2000 |
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An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JB.182.20.5653-5662.2000</identifier><identifier>PMID: 11004161</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>actinorhodin ; Amino Acid Sequence ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Base Sequence ; Cell Division ; Cells ; Gene Deletion ; Genetic Complementation Test ; Genetics ; Genetics and Molecular Biology ; iclR gene ; Microbiology ; Molecular Sequence Data ; Mutagenesis ; Plasmids ; Polymerase Chain Reaction ; Restriction Mapping ; Sequence Alignment ; Sequence Homology, Amino Acid ; Spores, Bacterial - physiology ; Spores, Bacterial - ultrastructure ; ssgA gene ; ssgR gene ; Streptomyces - cytology ; Streptomyces - genetics ; Streptomyces - physiology ; Streptomyces coelicolor ; whi gene</subject><ispartof>Journal of bacteriology, 2000-10, Vol.182 (20), p.5653-5662</ispartof><rights>Copyright American Society for Microbiology Oct 2000</rights><rights>Copyright © 2000, American Society for Microbiology 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-5b390ca02b10033a08fa9d4a55b4e9d594e5e8298e88251020606ea1735cdfb53</citedby><cites>FETCH-LOGICAL-c472t-5b390ca02b10033a08fa9d4a55b4e9d594e5e8298e88251020606ea1735cdfb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC94684/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC94684/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11004161$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Wezel, G P</creatorcontrib><creatorcontrib>van der Meulen, J</creatorcontrib><creatorcontrib>Kawamoto, S</creatorcontrib><creatorcontrib>Luiten, R G</creatorcontrib><creatorcontrib>Koerten, H K</creatorcontrib><creatorcontrib>Kraal, B</creatorcontrib><title>ssgA is essential for sporulation of Streptomyces coelicolor A3(2) and affects hyphal development by stimulating septum formation</title><title>Journal of bacteriology</title><addtitle>J Bacteriol</addtitle><description>The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.</description><subject>actinorhodin</subject><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - chemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Cell Division</subject><subject>Cells</subject><subject>Gene Deletion</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Genetics and Molecular Biology</subject><subject>iclR gene</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Restriction Mapping</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spores, Bacterial - physiology</subject><subject>Spores, Bacterial - ultrastructure</subject><subject>ssgA gene</subject><subject>ssgR gene</subject><subject>Streptomyces - cytology</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - physiology</subject><subject>Streptomyces coelicolor</subject><subject>whi gene</subject><issn>0021-9193</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtv1DAURi0EokPhLyCLBSqLDNfPOBKbacWrqsQCWFuOczOTKomDnVSaJf8cpx3xWlmWv-_4Xh1CKIMtY9y8vb7cMsO3HLZKK1EordcLwCOyYVCZQikBj8kGgLOiYpU4I89SugVgUir-lJwxBiCZZhvyM6X9jnaJYko4zp3raRsiTVOIS-_mLow0tPTrHHGaw3D0mKgP2Hc-9Dm2Exf8DXVjQ13bop8TPRynQ2Y0eId9mIaMpPWRprkb7nHjnqZMWob1l-Ge_5w8aV2f8MXpPCffP7z_dvWpuPny8fPV7qbwsuRzoWpRgXfA6zy7EA5M66pGOqVqiVWjKokKDa8MGsMVAw4aNDpWCuWbtlbinLx74E5LPWDj82jR9XaK3eDi0QbX2X9fxu5g9-HOVlIbmeuvT_UYfiyYZjt0yWPfuxHDkiwrDS-Vhhx89V_wNixxzKtZzkvQJcg1ZB5CPoaUIra_52BgV8X2-tJmxZaDXRXbVbFdFefqy7_3-FM8ORW_ANiipPs</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>van Wezel, G P</creator><creator>van der Meulen, J</creator><creator>Kawamoto, S</creator><creator>Luiten, R G</creator><creator>Koerten, H K</creator><creator>Kraal, B</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20001001</creationdate><title>ssgA is essential for sporulation of Streptomyces coelicolor A3(2) and affects hyphal development by stimulating septum formation</title><author>van Wezel, G P ; van der Meulen, J ; Kawamoto, S ; Luiten, R G ; Koerten, H K ; Kraal, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-5b390ca02b10033a08fa9d4a55b4e9d594e5e8298e88251020606ea1735cdfb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>actinorhodin</topic><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - chemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Cell Division</topic><topic>Cells</topic><topic>Gene Deletion</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Genetics and Molecular Biology</topic><topic>iclR gene</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Restriction Mapping</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spores, Bacterial - physiology</topic><topic>Spores, Bacterial - ultrastructure</topic><topic>ssgA gene</topic><topic>ssgR gene</topic><topic>Streptomyces - cytology</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - physiology</topic><topic>Streptomyces coelicolor</topic><topic>whi gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Wezel, G P</creatorcontrib><creatorcontrib>van der Meulen, J</creatorcontrib><creatorcontrib>Kawamoto, S</creatorcontrib><creatorcontrib>Luiten, R G</creatorcontrib><creatorcontrib>Koerten, H K</creatorcontrib><creatorcontrib>Kraal, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Wezel, G P</au><au>van der Meulen, J</au><au>Kawamoto, S</au><au>Luiten, R G</au><au>Koerten, H K</au><au>Kraal, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ssgA is essential for sporulation of Streptomyces coelicolor A3(2) and affects hyphal development by stimulating septum formation</atitle><jtitle>Journal of bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>182</volume><issue>20</issue><spage>5653</spage><epage>5662</epage><pages>5653-5662</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><coden>JOBAAY</coden><abstract>The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>11004161</pmid><doi>10.1128/JB.182.20.5653-5662.2000</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | actinorhodin Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Base Sequence Cell Division Cells Gene Deletion Genetic Complementation Test Genetics Genetics and Molecular Biology iclR gene Microbiology Molecular Sequence Data Mutagenesis Plasmids Polymerase Chain Reaction Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Spores, Bacterial - physiology Spores, Bacterial - ultrastructure ssgA gene ssgR gene Streptomyces - cytology Streptomyces - genetics Streptomyces - physiology Streptomyces coelicolor whi gene |
| title | ssgA is essential for sporulation of Streptomyces coelicolor A3(2) and affects hyphal development by stimulating septum formation |
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