Breaking down antibiotic resistance in methicillin-resistant Staphylococcus aureus : Combining antimicrobial photodynamic and antibiotic treatments
The widespread use of antibiotics drives the evolution of antimicrobial-resistant bacteria (ARB), threatening patients and healthcare professionals. Therefore, the development of novel strategies to combat resistance is recognized as a global healthcare priority. The two methods to combat ARB are de...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2022-09, Vol.119 (36), p.e2208378119-e2208378119 |
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| creator | Willis, Jace A Cheburkanov, Vsevolod Chen, Shaorong Soares, Jennifer M Kassab, Giulia Blanco, Kate C Bagnato, Vanderlei S de Figueiredo, Paul Yakovlev, Vladislav V |
| description | The widespread use of antibiotics drives the evolution of antimicrobial-resistant bacteria (ARB), threatening patients and healthcare professionals. Therefore, the development of novel strategies to combat resistance is recognized as a global healthcare priority. The two methods to combat ARB are development of new antibiotics or reduction in existing resistances. Development of novel antibiotics is a laborious and slow-progressing task that is no longer a safe reserve against looming risks. In this research, we suggest a method for reducing resistance to extend the efficacious lifetime of current antibiotics. Antimicrobial photodynamic therapy (aPDT) is used to generate reactive oxygen species (ROS) via the photoactivation of a photosensitizer. ROS then nonspecifically damage cellular components, leading to general impairment and cell death. Here, we test the hypothesis that concurrent treatment of bacteria with antibiotics and aPDT achieves an additive effect in the elimination of ARB. Performing aPDT with the photosensitizer methylene blue in combination with antibiotics chloramphenicol and tetracycline results in significant reductions in resistance for two methicillin-resistant
(MRSA) strains, USA300 and RN4220. Additional resistant
strain and antibiotic combinations reveal similar results. Taken together, these results suggest that concurrent aPDT consistently decreases
resistance by improving susceptibility to antibiotic treatment. In turn, this development exhibits an alternative to overcome some of the growing MRSA challenge. |
| doi_str_mv | 10.1073/pnas.2208378119 |
| format | Article |
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(MRSA) strains, USA300 and RN4220. Additional resistant
strain and antibiotic combinations reveal similar results. Taken together, these results suggest that concurrent aPDT consistently decreases
resistance by improving susceptibility to antibiotic treatment. In turn, this development exhibits an alternative to overcome some of the growing MRSA challenge.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.2208378119</identifier><identifier>PMID: 36037346</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Antiinfectives and antibacterials ; Bacteria ; Biological Sciences ; Breaking down ; Cell death ; Chloramphenicol ; Chloromycetin ; Drug resistance ; Drug Resistance, Microbial - drug effects ; Drug Resistance, Microbial - radiation effects ; Global health ; Health care ; Humans ; Methicillin ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - radiation effects ; Methylene blue ; Penicillin ; Photoactivation ; Photochemotherapy ; Photodynamic therapy ; Photosensitizing Agents - pharmacology ; Public health ; Reactive oxygen species ; Reactive Oxygen Species - pharmacology ; Staphylococcus aureus ; Staphylococcus infections</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2022-09, Vol.119 (36), p.e2208378119-e2208378119</ispartof><rights>Copyright National Academy of Sciences Sep 6, 2022</rights><rights>Copyright © 2022 the Author(s). Published by PNAS. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-b8a3aea229784f4c70e91f698d6c66905252e7569dc46416e3888f1ce58101303</citedby><cites>FETCH-LOGICAL-c421t-b8a3aea229784f4c70e91f698d6c66905252e7569dc46416e3888f1ce58101303</cites><orcidid>0000-0003-4833-239X ; 0000-0002-9055-7763 ; 0000-0002-4557-1013 ; 0000-0003-0361-9725</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457041/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457041/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36037346$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Willis, Jace A</creatorcontrib><creatorcontrib>Cheburkanov, Vsevolod</creatorcontrib><creatorcontrib>Chen, Shaorong</creatorcontrib><creatorcontrib>Soares, Jennifer M</creatorcontrib><creatorcontrib>Kassab, Giulia</creatorcontrib><creatorcontrib>Blanco, Kate C</creatorcontrib><creatorcontrib>Bagnato, Vanderlei S</creatorcontrib><creatorcontrib>de Figueiredo, Paul</creatorcontrib><creatorcontrib>Yakovlev, Vladislav V</creatorcontrib><title>Breaking down antibiotic resistance in methicillin-resistant Staphylococcus aureus : Combining antimicrobial photodynamic and antibiotic treatments</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The widespread use of antibiotics drives the evolution of antimicrobial-resistant bacteria (ARB), threatening patients and healthcare professionals. Therefore, the development of novel strategies to combat resistance is recognized as a global healthcare priority. The two methods to combat ARB are development of new antibiotics or reduction in existing resistances. Development of novel antibiotics is a laborious and slow-progressing task that is no longer a safe reserve against looming risks. In this research, we suggest a method for reducing resistance to extend the efficacious lifetime of current antibiotics. Antimicrobial photodynamic therapy (aPDT) is used to generate reactive oxygen species (ROS) via the photoactivation of a photosensitizer. ROS then nonspecifically damage cellular components, leading to general impairment and cell death. Here, we test the hypothesis that concurrent treatment of bacteria with antibiotics and aPDT achieves an additive effect in the elimination of ARB. Performing aPDT with the photosensitizer methylene blue in combination with antibiotics chloramphenicol and tetracycline results in significant reductions in resistance for two methicillin-resistant
(MRSA) strains, USA300 and RN4220. Additional resistant
strain and antibiotic combinations reveal similar results. Taken together, these results suggest that concurrent aPDT consistently decreases
resistance by improving susceptibility to antibiotic treatment. In turn, this development exhibits an alternative to overcome some of the growing MRSA challenge.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacteria</subject><subject>Biological Sciences</subject><subject>Breaking down</subject><subject>Cell death</subject><subject>Chloramphenicol</subject><subject>Chloromycetin</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial - drug effects</subject><subject>Drug Resistance, Microbial - radiation effects</subject><subject>Global health</subject><subject>Health care</subject><subject>Humans</subject><subject>Methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus - drug effects</subject><subject>Methicillin-Resistant Staphylococcus aureus - radiation effects</subject><subject>Methylene blue</subject><subject>Penicillin</subject><subject>Photoactivation</subject><subject>Photochemotherapy</subject><subject>Photodynamic therapy</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Public health</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - pharmacology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUlv1TAUhS0Eoo_Cmh2KxIZN2ushHlgglScmqRILYG05jtPnktjBdkDvd_QP108dKKyu5PP53OEg9BLDCQZBT5dg8gkhIKmQGKtHaINB4ZYzBY_RBoCIVjLCjtCznC8BQHUSnqIjyoEKyvgGXb1Pzvz04aIZ4p_QmFB872Pxtkku-1xMsK7xoZld2Xnrp8mH9k4pzbdilt1-ijZau-bGrMnV8rbZxrn34eB6MJy9TbH3ZmqWXSxx2AdTn6o0POxX6iBldqHk5-jJaKbsXtzWY_Tj44fv28_t-ddPX7Zn561lBJe2l4YaZwhRQrKRWQFO4ZErOXDLuYKOdMSJjqvBMs4wd1RKOWLrOokBU6DH6N2N77L2sxts7Z3MpJfkZ5P2Ohqv_1WC3-mL-Fsr1glguBq8uTVI8dfqctGzz9ZNkwkurlkTAZJ0ikhZ0df_oZdxTaGuVylMiOgkF5U6vaHqwXJObrwfBoM-BK4Pgeu_gdcfrx7ucM_fJUyvAdzeq2g</recordid><startdate>20220906</startdate><enddate>20220906</enddate><creator>Willis, Jace A</creator><creator>Cheburkanov, Vsevolod</creator><creator>Chen, Shaorong</creator><creator>Soares, Jennifer M</creator><creator>Kassab, Giulia</creator><creator>Blanco, Kate C</creator><creator>Bagnato, Vanderlei S</creator><creator>de Figueiredo, Paul</creator><creator>Yakovlev, Vladislav V</creator><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4833-239X</orcidid><orcidid>https://orcid.org/0000-0002-9055-7763</orcidid><orcidid>https://orcid.org/0000-0002-4557-1013</orcidid><orcidid>https://orcid.org/0000-0003-0361-9725</orcidid></search><sort><creationdate>20220906</creationdate><title>Breaking down antibiotic resistance in methicillin-resistant Staphylococcus aureus : Combining antimicrobial photodynamic and antibiotic treatments</title><author>Willis, Jace A ; 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Therefore, the development of novel strategies to combat resistance is recognized as a global healthcare priority. The two methods to combat ARB are development of new antibiotics or reduction in existing resistances. Development of novel antibiotics is a laborious and slow-progressing task that is no longer a safe reserve against looming risks. In this research, we suggest a method for reducing resistance to extend the efficacious lifetime of current antibiotics. Antimicrobial photodynamic therapy (aPDT) is used to generate reactive oxygen species (ROS) via the photoactivation of a photosensitizer. ROS then nonspecifically damage cellular components, leading to general impairment and cell death. Here, we test the hypothesis that concurrent treatment of bacteria with antibiotics and aPDT achieves an additive effect in the elimination of ARB. Performing aPDT with the photosensitizer methylene blue in combination with antibiotics chloramphenicol and tetracycline results in significant reductions in resistance for two methicillin-resistant
(MRSA) strains, USA300 and RN4220. Additional resistant
strain and antibiotic combinations reveal similar results. Taken together, these results suggest that concurrent aPDT consistently decreases
resistance by improving susceptibility to antibiotic treatment. In turn, this development exhibits an alternative to overcome some of the growing MRSA challenge.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>36037346</pmid><doi>10.1073/pnas.2208378119</doi><orcidid>https://orcid.org/0000-0003-4833-239X</orcidid><orcidid>https://orcid.org/0000-0002-9055-7763</orcidid><orcidid>https://orcid.org/0000-0002-4557-1013</orcidid><orcidid>https://orcid.org/0000-0003-0361-9725</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antiinfectives and antibacterials Bacteria Biological Sciences Breaking down Cell death Chloramphenicol Chloromycetin Drug resistance Drug Resistance, Microbial - drug effects Drug Resistance, Microbial - radiation effects Global health Health care Humans Methicillin Methicillin-Resistant Staphylococcus aureus - drug effects Methicillin-Resistant Staphylococcus aureus - radiation effects Methylene blue Penicillin Photoactivation Photochemotherapy Photodynamic therapy Photosensitizing Agents - pharmacology Public health Reactive oxygen species Reactive Oxygen Species - pharmacology Staphylococcus aureus Staphylococcus infections |
| title | Breaking down antibiotic resistance in methicillin-resistant Staphylococcus aureus : Combining antimicrobial photodynamic and antibiotic treatments |
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