eif4ebp3l—A New Affector of Zebrafish Angiogenesis and Heart Regeneration?
The eukaryotic initiation factor 4E binding protein (4E-BP) family is involved in translational control of cell proliferation and pro-angiogenic factors. The zebrafish eukaryotic initiation factor 4E binding protein 3 like (eif4ebp3l) is a member of the 4E-BPs and responsible for activity-dependent...
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| Veröffentlicht in: | International journal of molecular sciences 2022-09, Vol.23 (17), p.10075 |
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| creator | Born, Lisa I. Andree, Theresa Frank, Svenja Hübner, Judith Link, Sandra Langheine, Marion Charlet, Anne Esser, Jennifer S. Brehm, Ralph Moser, Martin |
| description | The eukaryotic initiation factor 4E binding protein (4E-BP) family is involved in translational control of cell proliferation and pro-angiogenic factors. The zebrafish eukaryotic initiation factor 4E binding protein 3 like (eif4ebp3l) is a member of the 4E-BPs and responsible for activity-dependent myofibrillogenesis, but whether it affects cardiomyocyte (CM) proliferation or heart regeneration is unclear. We examined eif4ebp3l during zebrafish vascular development and heart regeneration post cryoinjury in adult zebrafish. Using morpholino injections we induced silencing of eif4ebp3l in zebrafish embryos, which led to increased angiogenesis at 94 h post fertilization (hpf). For investigation of eif4ebp3l in cardiac regeneration, zebrafish hearts were subjected to cryoinjury. Regenerating hearts were analyzed at different time points post-cryoinjury for expression of eif4ebp3l by in situ hybridization and showed strongly decreased eif4ebp3l expression in the injured area. We established a transgenic zebrafish strain, which overexpressed eif4ebp3l under the control of a heat-shock dependent promotor. Overexpression of eif4ebp3l during zebrafish heart regeneration caused only macroscopically a reduced amount of fibrin at the site of injury. Overall, these findings demonstrate that silencing of eif4ebp3l has pro-angiogenic properties in zebrafish vascular development and when eif4ebp3l is overexpressed, fibrin deposition tends to be altered in zebrafish cardiac regeneration after cryoinjury. |
| doi_str_mv | 10.3390/ijms231710075 |
| format | Article |
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The zebrafish eukaryotic initiation factor 4E binding protein 3 like (eif4ebp3l) is a member of the 4E-BPs and responsible for activity-dependent myofibrillogenesis, but whether it affects cardiomyocyte (CM) proliferation or heart regeneration is unclear. We examined eif4ebp3l during zebrafish vascular development and heart regeneration post cryoinjury in adult zebrafish. Using morpholino injections we induced silencing of eif4ebp3l in zebrafish embryos, which led to increased angiogenesis at 94 h post fertilization (hpf). For investigation of eif4ebp3l in cardiac regeneration, zebrafish hearts were subjected to cryoinjury. Regenerating hearts were analyzed at different time points post-cryoinjury for expression of eif4ebp3l by in situ hybridization and showed strongly decreased eif4ebp3l expression in the injured area. We established a transgenic zebrafish strain, which overexpressed eif4ebp3l under the control of a heat-shock dependent promotor. Overexpression of eif4ebp3l during zebrafish heart regeneration caused only macroscopically a reduced amount of fibrin at the site of injury. Overall, these findings demonstrate that silencing of eif4ebp3l has pro-angiogenic properties in zebrafish vascular development and when eif4ebp3l is overexpressed, fibrin deposition tends to be altered in zebrafish cardiac regeneration after cryoinjury.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms231710075</identifier><identifier>PMID: 36077472</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Angiogenesis ; Cardiomyocytes ; Cell proliferation ; Cryoinjury ; Danio rerio ; Embryos ; Fertilization ; Fibrin ; Gene expression ; Genotype & phenotype ; Growth factors ; Heart ; Heart failure ; Hybridization ; Initiation factor eIF-4E ; Injury prevention ; Proteins ; Tumorigenesis ; Zebrafish</subject><ispartof>International journal of molecular sciences, 2022-09, Vol.23 (17), p.10075</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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The zebrafish eukaryotic initiation factor 4E binding protein 3 like (eif4ebp3l) is a member of the 4E-BPs and responsible for activity-dependent myofibrillogenesis, but whether it affects cardiomyocyte (CM) proliferation or heart regeneration is unclear. We examined eif4ebp3l during zebrafish vascular development and heart regeneration post cryoinjury in adult zebrafish. Using morpholino injections we induced silencing of eif4ebp3l in zebrafish embryos, which led to increased angiogenesis at 94 h post fertilization (hpf). For investigation of eif4ebp3l in cardiac regeneration, zebrafish hearts were subjected to cryoinjury. Regenerating hearts were analyzed at different time points post-cryoinjury for expression of eif4ebp3l by in situ hybridization and showed strongly decreased eif4ebp3l expression in the injured area. We established a transgenic zebrafish strain, which overexpressed eif4ebp3l under the control of a heat-shock dependent promotor. 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Overall, these findings demonstrate that silencing of eif4ebp3l has pro-angiogenic properties in zebrafish vascular development and when eif4ebp3l is overexpressed, fibrin deposition tends to be altered in zebrafish cardiac regeneration after cryoinjury.</description><subject>Angiogenesis</subject><subject>Cardiomyocytes</subject><subject>Cell proliferation</subject><subject>Cryoinjury</subject><subject>Danio rerio</subject><subject>Embryos</subject><subject>Fertilization</subject><subject>Fibrin</subject><subject>Gene expression</subject><subject>Genotype & phenotype</subject><subject>Growth factors</subject><subject>Heart</subject><subject>Heart failure</subject><subject>Hybridization</subject><subject>Initiation factor eIF-4E</subject><subject>Injury prevention</subject><subject>Proteins</subject><subject>Tumorigenesis</subject><subject>Zebrafish</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc9Kw0AQhxdRrFaP3gNevET3_yYXpRS1QlEQvXhZNslsuyXN1t1E8eZD-IQ-iS0tYj3NMPPxMcMPoROCzxnL8YWbzSNlRBGMldhBB4RTmmIs1e6fvocOY5xhTBkV-T7qMYmV4ooeoDE4y6FYsPr782uQ3MN7MrAWytaHxNvkBYpgrIvTZNBMnJ9AA9HFxDRVMgIT2uQRVrNgWuebqyO0Z00d4XhT--j55vppOErHD7d3w8E4LRnP2pSwklCaU8uJMgXwjOeGlVZiWnFTCVIIooAJUeSkwtJKTklFJK4yQUpqZc766HLtXXTFHKoSmjaYWi-Cm5vwob1xenvTuKme-DedcyG5xEvB2UYQ_GsHsdVzF0uoa9OA76KmitBMSInFEj39h858F5rleyuKcI4FzZZUuqbK4GMMYH-PIVivctJbObEfHR2EHQ</recordid><startdate>20220903</startdate><enddate>20220903</enddate><creator>Born, Lisa I.</creator><creator>Andree, Theresa</creator><creator>Frank, Svenja</creator><creator>Hübner, Judith</creator><creator>Link, Sandra</creator><creator>Langheine, Marion</creator><creator>Charlet, Anne</creator><creator>Esser, Jennifer S.</creator><creator>Brehm, Ralph</creator><creator>Moser, Martin</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9180-1863</orcidid><orcidid>https://orcid.org/0000-0002-8494-2016</orcidid><orcidid>https://orcid.org/0000-0002-0489-7594</orcidid></search><sort><creationdate>20220903</creationdate><title>eif4ebp3l—A New Affector of Zebrafish Angiogenesis and Heart Regeneration?</title><author>Born, Lisa I. ; 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The zebrafish eukaryotic initiation factor 4E binding protein 3 like (eif4ebp3l) is a member of the 4E-BPs and responsible for activity-dependent myofibrillogenesis, but whether it affects cardiomyocyte (CM) proliferation or heart regeneration is unclear. We examined eif4ebp3l during zebrafish vascular development and heart regeneration post cryoinjury in adult zebrafish. Using morpholino injections we induced silencing of eif4ebp3l in zebrafish embryos, which led to increased angiogenesis at 94 h post fertilization (hpf). For investigation of eif4ebp3l in cardiac regeneration, zebrafish hearts were subjected to cryoinjury. Regenerating hearts were analyzed at different time points post-cryoinjury for expression of eif4ebp3l by in situ hybridization and showed strongly decreased eif4ebp3l expression in the injured area. We established a transgenic zebrafish strain, which overexpressed eif4ebp3l under the control of a heat-shock dependent promotor. Overexpression of eif4ebp3l during zebrafish heart regeneration caused only macroscopically a reduced amount of fibrin at the site of injury. Overall, these findings demonstrate that silencing of eif4ebp3l has pro-angiogenic properties in zebrafish vascular development and when eif4ebp3l is overexpressed, fibrin deposition tends to be altered in zebrafish cardiac regeneration after cryoinjury.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36077472</pmid><doi>10.3390/ijms231710075</doi><orcidid>https://orcid.org/0000-0001-9180-1863</orcidid><orcidid>https://orcid.org/0000-0002-8494-2016</orcidid><orcidid>https://orcid.org/0000-0002-0489-7594</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Angiogenesis Cardiomyocytes Cell proliferation Cryoinjury Danio rerio Embryos Fertilization Fibrin Gene expression Genotype & phenotype Growth factors Heart Heart failure Hybridization Initiation factor eIF-4E Injury prevention Proteins Tumorigenesis Zebrafish |
| title | eif4ebp3l—A New Affector of Zebrafish Angiogenesis and Heart Regeneration? |
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