Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs
The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and inf...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences 2022-09, Vol.23 (17), p.9873 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 17 |
| container_start_page | 9873 |
| container_title | International journal of molecular sciences |
| container_volume | 23 |
| creator | Ding, Mengfan Chen, Haiqin Yu, Renqiang Ross, Reynolds Paul Stanton, Catherine Zhang, Hao Yang, Bo Chen, Wei |
| description | The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and infant and maternal feces. Eleven pregnant women were recruited successfully. HBM; infant feces during colostrum, transition, and mature stages; and maternal feces within the mature stage were collected. sIgA-coated and sIgA-uncoated bacteria were separated with magnetic-activated cell sorting. Then, 16S rRNA sequencing, bifidobacterial groEL gene sequencing, and lactobacilli groEL gene sequencing were performed to analyze the bacterial community. PCoA revealed that the compositions of sIgA-coated and sIgA-uncoated bacteria were different among HBM and infant and maternal feces. Higher relative abundance of sIgA-uncoated Bifidobacterium was found in the three lactation stages in infant feces compared to the corresponding HBM, and a higher relative abundance of sIgA-uncoated Faecalibacterium was found in maternal feces compared to HBM and infant feces. For bifidobacterial community, sIgA-coated and sIgA-uncoated B. longum subsp. infantis and B. pseudocatenulatum was dominant in infant feces and maternal feces, respectively. The relative abundance of sIgA-uncoated B. longum subsp. infantis was significantly higher in infant feces compared to that in maternal feces. For the Lactobacillus community, L. paragasseri and L. mucosae were dominant in infant and maternal feces, respectively. HBM and infant and maternal feces showed distinct diversity and composition of both sIgA-coated and sIgA-uncoated bacteria at genus level. Infant and maternal feces showed similar composition of Bifidobacterium at species level. The same Bifidobacterium species could be detected both in sIgA-coated and -uncoated form. This article provided deeper understanding on the microbiota profile in HBM and infant and maternal feces. |
| doi_str_mv | 10.3390/ijms23179873 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9456154</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2712856585</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-41abf4fa6283794a16ebfbde07d3a28f4a4ed9fbbc5167e01d6ec28dcfb782ac3</originalsourceid><addsrcrecordid>eNpdkctKBDEQRYMovnd-QIMbF7bm1Ul6I-jgY8AXqFtDdTpxMswkmvQI7vwH_9AvsWUGUVdVlzpc6lYhtEPwAWM1PvTjaaaMyFpJtoTWCae0xFjI5V_9GtrIeYwxZbSqV9EaE1hKKsk6erwbQbJtAaEtrmMoFzIPn47LQYRuMSofgpmrEzCdTR4KH4ph6GzufLBFdMVV7EY2fb5_DIOD0BW34FPeQisOJtluL-omejg7vR9clJc358PB8WVpmKq7khNoHHcgqGKy5kCEbVzTWixbBlQ5Dty2tWsaUxEhLSatsIaq1rhGKgqGbaKjue_zrJna1tjQJZjo5-SnkN50BK__ToIf6af4qmteCVLx3mBvYZDiy6xPpac-GzuZQLBxlnV_LKoqUamqR3f_oeM4S6GP900RLoTCsqf255RJMedk3c8yBOvvx-nfj2NfIA2MMA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2711466807</pqid></control><display><type>article</type><title>Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Ding, Mengfan ; Chen, Haiqin ; Yu, Renqiang ; Ross, Reynolds Paul ; Stanton, Catherine ; Zhang, Hao ; Yang, Bo ; Chen, Wei</creator><creatorcontrib>Ding, Mengfan ; Chen, Haiqin ; Yu, Renqiang ; Ross, Reynolds Paul ; Stanton, Catherine ; Zhang, Hao ; Yang, Bo ; Chen, Wei</creatorcontrib><description>The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and infant and maternal feces. Eleven pregnant women were recruited successfully. HBM; infant feces during colostrum, transition, and mature stages; and maternal feces within the mature stage were collected. sIgA-coated and sIgA-uncoated bacteria were separated with magnetic-activated cell sorting. Then, 16S rRNA sequencing, bifidobacterial groEL gene sequencing, and lactobacilli groEL gene sequencing were performed to analyze the bacterial community. PCoA revealed that the compositions of sIgA-coated and sIgA-uncoated bacteria were different among HBM and infant and maternal feces. Higher relative abundance of sIgA-uncoated Bifidobacterium was found in the three lactation stages in infant feces compared to the corresponding HBM, and a higher relative abundance of sIgA-uncoated Faecalibacterium was found in maternal feces compared to HBM and infant feces. For bifidobacterial community, sIgA-coated and sIgA-uncoated B. longum subsp. infantis and B. pseudocatenulatum was dominant in infant feces and maternal feces, respectively. The relative abundance of sIgA-uncoated B. longum subsp. infantis was significantly higher in infant feces compared to that in maternal feces. For the Lactobacillus community, L. paragasseri and L. mucosae were dominant in infant and maternal feces, respectively. HBM and infant and maternal feces showed distinct diversity and composition of both sIgA-coated and sIgA-uncoated bacteria at genus level. Infant and maternal feces showed similar composition of Bifidobacterium at species level. The same Bifidobacterium species could be detected both in sIgA-coated and -uncoated form. This article provided deeper understanding on the microbiota profile in HBM and infant and maternal feces.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms23179873</identifier><identifier>PMID: 36077271</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Abundance ; Babies ; Bacteria ; Bifidobacterium ; Breast milk ; Breastfeeding & lactation ; Coatings ; Colostrum ; Composition ; DNA sequencing ; Feces ; GroEL gene ; Infants ; Intestinal microflora ; Intestine ; Lactation ; Lactobacilli ; Microbiota ; Milk ; Relative abundance ; rRNA 16S</subject><ispartof>International journal of molecular sciences, 2022-09, Vol.23 (17), p.9873</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-41abf4fa6283794a16ebfbde07d3a28f4a4ed9fbbc5167e01d6ec28dcfb782ac3</citedby><cites>FETCH-LOGICAL-c389t-41abf4fa6283794a16ebfbde07d3a28f4a4ed9fbbc5167e01d6ec28dcfb782ac3</cites><orcidid>0000-0003-4876-8839 ; 0000-0002-6724-7011 ; 0000-0002-1347-3718 ; 0000-0001-6529-0158</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456154/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456154/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids></links><search><creatorcontrib>Ding, Mengfan</creatorcontrib><creatorcontrib>Chen, Haiqin</creatorcontrib><creatorcontrib>Yu, Renqiang</creatorcontrib><creatorcontrib>Ross, Reynolds Paul</creatorcontrib><creatorcontrib>Stanton, Catherine</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><title>Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs</title><title>International journal of molecular sciences</title><description>The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and infant and maternal feces. Eleven pregnant women were recruited successfully. HBM; infant feces during colostrum, transition, and mature stages; and maternal feces within the mature stage were collected. sIgA-coated and sIgA-uncoated bacteria were separated with magnetic-activated cell sorting. Then, 16S rRNA sequencing, bifidobacterial groEL gene sequencing, and lactobacilli groEL gene sequencing were performed to analyze the bacterial community. PCoA revealed that the compositions of sIgA-coated and sIgA-uncoated bacteria were different among HBM and infant and maternal feces. Higher relative abundance of sIgA-uncoated Bifidobacterium was found in the three lactation stages in infant feces compared to the corresponding HBM, and a higher relative abundance of sIgA-uncoated Faecalibacterium was found in maternal feces compared to HBM and infant feces. For bifidobacterial community, sIgA-coated and sIgA-uncoated B. longum subsp. infantis and B. pseudocatenulatum was dominant in infant feces and maternal feces, respectively. The relative abundance of sIgA-uncoated B. longum subsp. infantis was significantly higher in infant feces compared to that in maternal feces. For the Lactobacillus community, L. paragasseri and L. mucosae were dominant in infant and maternal feces, respectively. HBM and infant and maternal feces showed distinct diversity and composition of both sIgA-coated and sIgA-uncoated bacteria at genus level. Infant and maternal feces showed similar composition of Bifidobacterium at species level. The same Bifidobacterium species could be detected both in sIgA-coated and -uncoated form. This article provided deeper understanding on the microbiota profile in HBM and infant and maternal feces.</description><subject>Abundance</subject><subject>Babies</subject><subject>Bacteria</subject><subject>Bifidobacterium</subject><subject>Breast milk</subject><subject>Breastfeeding & lactation</subject><subject>Coatings</subject><subject>Colostrum</subject><subject>Composition</subject><subject>DNA sequencing</subject><subject>Feces</subject><subject>GroEL gene</subject><subject>Infants</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Lactation</subject><subject>Lactobacilli</subject><subject>Microbiota</subject><subject>Milk</subject><subject>Relative abundance</subject><subject>rRNA 16S</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkctKBDEQRYMovnd-QIMbF7bm1Ul6I-jgY8AXqFtDdTpxMswkmvQI7vwH_9AvsWUGUVdVlzpc6lYhtEPwAWM1PvTjaaaMyFpJtoTWCae0xFjI5V_9GtrIeYwxZbSqV9EaE1hKKsk6erwbQbJtAaEtrmMoFzIPn47LQYRuMSofgpmrEzCdTR4KH4ph6GzufLBFdMVV7EY2fb5_DIOD0BW34FPeQisOJtluL-omejg7vR9clJc358PB8WVpmKq7khNoHHcgqGKy5kCEbVzTWixbBlQ5Dty2tWsaUxEhLSatsIaq1rhGKgqGbaKjue_zrJna1tjQJZjo5-SnkN50BK__ToIf6af4qmteCVLx3mBvYZDiy6xPpac-GzuZQLBxlnV_LKoqUamqR3f_oeM4S6GP900RLoTCsqf255RJMedk3c8yBOvvx-nfj2NfIA2MMA</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Ding, Mengfan</creator><creator>Chen, Haiqin</creator><creator>Yu, Renqiang</creator><creator>Ross, Reynolds Paul</creator><creator>Stanton, Catherine</creator><creator>Zhang, Hao</creator><creator>Yang, Bo</creator><creator>Chen, Wei</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4876-8839</orcidid><orcidid>https://orcid.org/0000-0002-6724-7011</orcidid><orcidid>https://orcid.org/0000-0002-1347-3718</orcidid><orcidid>https://orcid.org/0000-0001-6529-0158</orcidid></search><sort><creationdate>20220901</creationdate><title>Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs</title><author>Ding, Mengfan ; Chen, Haiqin ; Yu, Renqiang ; Ross, Reynolds Paul ; Stanton, Catherine ; Zhang, Hao ; Yang, Bo ; Chen, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-41abf4fa6283794a16ebfbde07d3a28f4a4ed9fbbc5167e01d6ec28dcfb782ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abundance</topic><topic>Babies</topic><topic>Bacteria</topic><topic>Bifidobacterium</topic><topic>Breast milk</topic><topic>Breastfeeding & lactation</topic><topic>Coatings</topic><topic>Colostrum</topic><topic>Composition</topic><topic>DNA sequencing</topic><topic>Feces</topic><topic>GroEL gene</topic><topic>Infants</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Lactation</topic><topic>Lactobacilli</topic><topic>Microbiota</topic><topic>Milk</topic><topic>Relative abundance</topic><topic>rRNA 16S</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Mengfan</creatorcontrib><creatorcontrib>Chen, Haiqin</creatorcontrib><creatorcontrib>Yu, Renqiang</creatorcontrib><creatorcontrib>Ross, Reynolds Paul</creatorcontrib><creatorcontrib>Stanton, Catherine</creatorcontrib><creatorcontrib>Zhang, Hao</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Chen, Wei</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Mengfan</au><au>Chen, Haiqin</au><au>Yu, Renqiang</au><au>Ross, Reynolds Paul</au><au>Stanton, Catherine</au><au>Zhang, Hao</au><au>Yang, Bo</au><au>Chen, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs</atitle><jtitle>International journal of molecular sciences</jtitle><date>2022-09-01</date><risdate>2022</risdate><volume>23</volume><issue>17</issue><spage>9873</spage><pages>9873-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The infant gut microbiota is critical for promoting and maintaining early-life health. The study aimed to analyze the composition of sIgA-coated and sIgA-uncoated bacterial communities at genus level and lactobacilli and bifidobacterial communities at species level in human breast milk (HBM) and infant and maternal feces. Eleven pregnant women were recruited successfully. HBM; infant feces during colostrum, transition, and mature stages; and maternal feces within the mature stage were collected. sIgA-coated and sIgA-uncoated bacteria were separated with magnetic-activated cell sorting. Then, 16S rRNA sequencing, bifidobacterial groEL gene sequencing, and lactobacilli groEL gene sequencing were performed to analyze the bacterial community. PCoA revealed that the compositions of sIgA-coated and sIgA-uncoated bacteria were different among HBM and infant and maternal feces. Higher relative abundance of sIgA-uncoated Bifidobacterium was found in the three lactation stages in infant feces compared to the corresponding HBM, and a higher relative abundance of sIgA-uncoated Faecalibacterium was found in maternal feces compared to HBM and infant feces. For bifidobacterial community, sIgA-coated and sIgA-uncoated B. longum subsp. infantis and B. pseudocatenulatum was dominant in infant feces and maternal feces, respectively. The relative abundance of sIgA-uncoated B. longum subsp. infantis was significantly higher in infant feces compared to that in maternal feces. For the Lactobacillus community, L. paragasseri and L. mucosae were dominant in infant and maternal feces, respectively. HBM and infant and maternal feces showed distinct diversity and composition of both sIgA-coated and sIgA-uncoated bacteria at genus level. Infant and maternal feces showed similar composition of Bifidobacterium at species level. The same Bifidobacterium species could be detected both in sIgA-coated and -uncoated form. This article provided deeper understanding on the microbiota profile in HBM and infant and maternal feces.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36077271</pmid><doi>10.3390/ijms23179873</doi><orcidid>https://orcid.org/0000-0003-4876-8839</orcidid><orcidid>https://orcid.org/0000-0002-6724-7011</orcidid><orcidid>https://orcid.org/0000-0002-1347-3718</orcidid><orcidid>https://orcid.org/0000-0001-6529-0158</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2022-09, Vol.23 (17), p.9873 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9456154 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Abundance Babies Bacteria Bifidobacterium Breast milk Breastfeeding & lactation Coatings Colostrum Composition DNA sequencing Feces GroEL gene Infants Intestinal microflora Intestine Lactation Lactobacilli Microbiota Milk Relative abundance rRNA 16S |
| title | Shared and Non-Shared sIgA-Coated and -Uncoated Bacteria in Intestine of Mother–Infant Pairs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T07%3A15%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Shared%20and%20Non-Shared%20sIgA-Coated%20and%20-Uncoated%20Bacteria%20in%20Intestine%20of%20Mother%E2%80%93Infant%20Pairs&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Ding,%20Mengfan&rft.date=2022-09-01&rft.volume=23&rft.issue=17&rft.spage=9873&rft.pages=9873-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms23179873&rft_dat=%3Cproquest_pubme%3E2712856585%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2711466807&rft_id=info:pmid/36077271&rfr_iscdi=true |