A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states

Microglia are emerging as key drivers of neurological diseases. However, we lack a systematic understanding of the underlying mechanisms. Here, we present a screening platform to systematically elucidate functional consequences of genetic perturbations in human induced pluripotent stem cell-derived...

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Veröffentlicht in:	Nature neuroscience 2022-09, Vol.25 (9), p.1149-1162
Hauptverfasser:	Dräger, Nina M., Sattler, Sydney M., Huang, Cindy Tzu-Ling, Teter, Olivia M., Leng, Kun, Hashemi, Sayed Hadi, Hong, Jason, Aviles, Giovanni, Clelland, Claire D., Zhan, Lihong, Udeochu, Joe C., Kodama, Lay, Singleton, Andrew B., Nalls, Mike A., Ichida, Justin, Ward, Michael E., Faghri, Faraz, Gan, Li, Kampmann, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2163 631/208/191 631/378/2596/1953 631/532/1360 Animal Genetics and Genomics Behavioral Sciences Biological Techniques Biomedical and Life Sciences Biomedicine Brain - metabolism Clustered Regularly Interspaced Short Palindromic Repeats - genetics Colony-stimulating factor CRISPR Disease Gene sequencing Genes Genomes Humans Induced Pluripotent Stem Cells - metabolism Macrophage colony-stimulating factor Microglia Microglia - metabolism Neurobiology Neurodegeneration Neurological diseases Neurosciences Osteopontin Perturbation Phagocytosis Phagocytosis - genetics Pluripotency Stem cells Therapeutic targets Transcription activation Transcription factors
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