Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study

Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study

1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) lipase assays are used to measure lipase activity in the diagnosis of pancreatitis. The effect of hepatic lipases released from damaged hepatocytes on serum DGGR lipase activity has not been reported, to our knowledge. We i...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of veterinary diagnostic investigation 2022-09, Vol.34 (5), p.854-858
Hauptverfasser: Thomson, James M., Williams, Tim L.
Format: Artikel
Sprache:eng
Schlagworte:
Brief Reports
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 858
container_issue 5
container_start_page 854
container_title Journal of veterinary diagnostic investigation
container_volume 34
creator Thomson, James M.
Williams, Tim L.
description 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) lipase assays are used to measure lipase activity in the diagnosis of pancreatitis. The effect of hepatic lipases released from damaged hepatocytes on serum DGGR lipase activity has not been reported, to our knowledge. We identified dogs with histologically confirmed liver lesions and concurrent unremarkable pancreatic histology, and dogs with no histologic evidence of hepatic or pancreatic disease. Dogs with relevant comorbidities were excluded. The hepatopathy group (n = 7) included 4 dogs with inflammatory hepatopathies, 2 with hepatic neoplasia, and 1 with unspecified (non-inflammatory, non-neoplastic) hepatopathy. The control group (n = 5) included one dog each with enteritis, subcutaneous hemangiosarcoma, hydrocephalus, myelomalacia, and tetanus. A Mann–Whitney U test compared selected biochemical parameters including serum DGGR lipase, alkaline phosphatase, alanine aminotransferase, and amylase activities, with statistical significance defined as p ≤ 0.05. Data are presented as median and range. Serum DGGR lipase activity (RI:
doi_str_mv 10.1177/10406387221106401
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9446298</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_10406387221106401</sage_id><sourcerecordid>2681816414</sourcerecordid><originalsourceid>FETCH-LOGICAL-c345t-4e730d8fd6dfc2733e42fb8c68b1bf2074c275494b0b456ece7748faafe97eae3</originalsourceid><addsrcrecordid>eNp9kU1r3DAQhkVoSdIkPyA3HXtxqi9Ldg6FkrbbQqAQ2rOQ5dGugtdyPHKK_321bAiUQg_DDDPv-wzMEHLN2Q3nxnzgTDEtGyME50wrxk_IOW-VrFQr9ZtSl3l1EJyRd4iPjNWiNvyUnMnaaMFZe07SA-Q54QQ-x2egbnTDihFpCtQhJh9djmmkHeTfACPdweRyKrFbi7anCPOyp583mwc6xMlhIRxAMa80jrRPW7yljk5xSJliXvr1krwNbkC4eskX5NfXLz_vvlX3Pzbf7z7dV16qOlcKjGR9E3rdBy-MlKBE6Bqvm453QTCjSrdWrepYp2oNHoxRTXAuQGvAgbwgH4_caen20HsY8-wGO81x7-bVJhft35Mx7uw2PdtWKS3apgDevwDm9LQAZruP6GEY3AhpQSt0wxuuFVdFyo9SXy6JM4TXNZzZw6PsP48qnpujB90W7GNa5nJ5_I_hD1ublIA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2681816414</pqid></control><display><type>article</type><title>Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study</title><source>Access via SAGE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Thomson, James M. ; Williams, Tim L.</creator><creatorcontrib>Thomson, James M. ; Williams, Tim L.</creatorcontrib><description>1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) lipase assays are used to measure lipase activity in the diagnosis of pancreatitis. The effect of hepatic lipases released from damaged hepatocytes on serum DGGR lipase activity has not been reported, to our knowledge. We identified dogs with histologically confirmed liver lesions and concurrent unremarkable pancreatic histology, and dogs with no histologic evidence of hepatic or pancreatic disease. Dogs with relevant comorbidities were excluded. The hepatopathy group (n = 7) included 4 dogs with inflammatory hepatopathies, 2 with hepatic neoplasia, and 1 with unspecified (non-inflammatory, non-neoplastic) hepatopathy. The control group (n = 5) included one dog each with enteritis, subcutaneous hemangiosarcoma, hydrocephalus, myelomalacia, and tetanus. A Mann–Whitney U test compared selected biochemical parameters including serum DGGR lipase, alkaline phosphatase, alanine aminotransferase, and amylase activities, with statistical significance defined as p ≤ 0.05. Data are presented as median and range. Serum DGGR lipase activity (RI: &lt;44 IU/L) was not different between the hepatopathy (52 IU/L; range: 27–85 IU/L) and control (37 IU/L, 25–105 IU/L; p = 0.947) groups. Serum amylase activity (RI: 256–1,610 IU/L) was significantly higher in the hepatopathy group (830 IU/L; 711–1,210 IU/L) than the control group (541 IU/L, 336–695 IU/L; p = 0.028). No association or correlation between serum DGGR lipase activity and hepatic lesions (based on histologic or biochemical findings) was identified, suggesting that clinically relevant changes in serum DGGR lipase activity may not be expected secondary to hepatopathy alone.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/10406387221106401</identifier><identifier>PMID: 35762109</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Brief Reports</subject><ispartof>Journal of veterinary diagnostic investigation, 2022-09, Vol.34 (5), p.854-858</ispartof><rights>2022 The Author(s)</rights><rights>2022 The Author(s) 2022 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-4e730d8fd6dfc2733e42fb8c68b1bf2074c275494b0b456ece7748faafe97eae3</citedby><cites>FETCH-LOGICAL-c345t-4e730d8fd6dfc2733e42fb8c68b1bf2074c275494b0b456ece7748faafe97eae3</cites><orcidid>0000-0002-9775-8285</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446298/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9446298/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,21819,27924,27925,43621,43622,53791,53793</link.rule.ids></links><search><creatorcontrib>Thomson, James M.</creatorcontrib><creatorcontrib>Williams, Tim L.</creatorcontrib><title>Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study</title><title>Journal of veterinary diagnostic investigation</title><description>1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) lipase assays are used to measure lipase activity in the diagnosis of pancreatitis. The effect of hepatic lipases released from damaged hepatocytes on serum DGGR lipase activity has not been reported, to our knowledge. We identified dogs with histologically confirmed liver lesions and concurrent unremarkable pancreatic histology, and dogs with no histologic evidence of hepatic or pancreatic disease. Dogs with relevant comorbidities were excluded. The hepatopathy group (n = 7) included 4 dogs with inflammatory hepatopathies, 2 with hepatic neoplasia, and 1 with unspecified (non-inflammatory, non-neoplastic) hepatopathy. The control group (n = 5) included one dog each with enteritis, subcutaneous hemangiosarcoma, hydrocephalus, myelomalacia, and tetanus. A Mann–Whitney U test compared selected biochemical parameters including serum DGGR lipase, alkaline phosphatase, alanine aminotransferase, and amylase activities, with statistical significance defined as p ≤ 0.05. Data are presented as median and range. Serum DGGR lipase activity (RI: &lt;44 IU/L) was not different between the hepatopathy (52 IU/L; range: 27–85 IU/L) and control (37 IU/L, 25–105 IU/L; p = 0.947) groups. Serum amylase activity (RI: 256–1,610 IU/L) was significantly higher in the hepatopathy group (830 IU/L; 711–1,210 IU/L) than the control group (541 IU/L, 336–695 IU/L; p = 0.028). No association or correlation between serum DGGR lipase activity and hepatic lesions (based on histologic or biochemical findings) was identified, suggesting that clinically relevant changes in serum DGGR lipase activity may not be expected secondary to hepatopathy alone.</description><subject>Brief Reports</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><recordid>eNp9kU1r3DAQhkVoSdIkPyA3HXtxqi9Ldg6FkrbbQqAQ2rOQ5dGugtdyPHKK_321bAiUQg_DDDPv-wzMEHLN2Q3nxnzgTDEtGyME50wrxk_IOW-VrFQr9ZtSl3l1EJyRd4iPjNWiNvyUnMnaaMFZe07SA-Q54QQ-x2egbnTDihFpCtQhJh9djmmkHeTfACPdweRyKrFbi7anCPOyp583mwc6xMlhIRxAMa80jrRPW7yljk5xSJliXvr1krwNbkC4eskX5NfXLz_vvlX3Pzbf7z7dV16qOlcKjGR9E3rdBy-MlKBE6Bqvm453QTCjSrdWrepYp2oNHoxRTXAuQGvAgbwgH4_caen20HsY8-wGO81x7-bVJhft35Mx7uw2PdtWKS3apgDevwDm9LQAZruP6GEY3AhpQSt0wxuuFVdFyo9SXy6JM4TXNZzZw6PsP48qnpujB90W7GNa5nJ5_I_hD1ublIA</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Thomson, James M.</creator><creator>Williams, Tim L.</creator><general>SAGE Publications</general><scope>AFRWT</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9775-8285</orcidid></search><sort><creationdate>20220901</creationdate><title>Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study</title><author>Thomson, James M. ; Williams, Tim L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-4e730d8fd6dfc2733e42fb8c68b1bf2074c275494b0b456ece7748faafe97eae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Brief Reports</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomson, James M.</creatorcontrib><creatorcontrib>Williams, Tim L.</creatorcontrib><collection>Sage Journals GOLD Open Access 2024</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomson, James M.</au><au>Williams, Tim L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><date>2022-09-01</date><risdate>2022</risdate><volume>34</volume><issue>5</issue><spage>854</spage><epage>858</epage><pages>854-858</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester (DGGR) lipase assays are used to measure lipase activity in the diagnosis of pancreatitis. The effect of hepatic lipases released from damaged hepatocytes on serum DGGR lipase activity has not been reported, to our knowledge. We identified dogs with histologically confirmed liver lesions and concurrent unremarkable pancreatic histology, and dogs with no histologic evidence of hepatic or pancreatic disease. Dogs with relevant comorbidities were excluded. The hepatopathy group (n = 7) included 4 dogs with inflammatory hepatopathies, 2 with hepatic neoplasia, and 1 with unspecified (non-inflammatory, non-neoplastic) hepatopathy. The control group (n = 5) included one dog each with enteritis, subcutaneous hemangiosarcoma, hydrocephalus, myelomalacia, and tetanus. A Mann–Whitney U test compared selected biochemical parameters including serum DGGR lipase, alkaline phosphatase, alanine aminotransferase, and amylase activities, with statistical significance defined as p ≤ 0.05. Data are presented as median and range. Serum DGGR lipase activity (RI: &lt;44 IU/L) was not different between the hepatopathy (52 IU/L; range: 27–85 IU/L) and control (37 IU/L, 25–105 IU/L; p = 0.947) groups. Serum amylase activity (RI: 256–1,610 IU/L) was significantly higher in the hepatopathy group (830 IU/L; 711–1,210 IU/L) than the control group (541 IU/L, 336–695 IU/L; p = 0.028). No association or correlation between serum DGGR lipase activity and hepatic lesions (based on histologic or biochemical findings) was identified, suggesting that clinically relevant changes in serum DGGR lipase activity may not be expected secondary to hepatopathy alone.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>35762109</pmid><doi>10.1177/10406387221106401</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-9775-8285</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1040-6387
ispartof Journal of veterinary diagnostic investigation, 2022-09, Vol.34 (5), p.854-858
issn 1040-6387
1943-4936
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9446298
source Access via SAGE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection
subjects Brief Reports
title Retrospective analysis of association between hepatopathy and serum DGGR lipase activity in dogs: a pilot study
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T21%3A30%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Retrospective%20analysis%20of%20association%20between%20hepatopathy%20and%20serum%20DGGR%20lipase%20activity%20in%20dogs:%20a%20pilot%20study&rft.jtitle=Journal%20of%20veterinary%20diagnostic%20investigation&rft.au=Thomson,%20James%20M.&rft.date=2022-09-01&rft.volume=34&rft.issue=5&rft.spage=854&rft.epage=858&rft.pages=854-858&rft.issn=1040-6387&rft.eissn=1943-4936&rft_id=info:doi/10.1177/10406387221106401&rft_dat=%3Cproquest_pubme%3E2681816414%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2681816414&rft_id=info:pmid/35762109&rft_sage_id=10.1177_10406387221106401&rfr_iscdi=true