Regulators of natural competence in Vibrio parahaemolyticus
Vibrio parahaemolyticus can degrade insoluble chitin with the help of chitinase enzymes that generate soluble N-acetyl glucosamine oligosaccharides (GlcNAc n ) to induce a state of natural competence for the uptake of extracellular DNA. In this study, we had evaluated the role of various regulatory...
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| Veröffentlicht in: | Brazilian journal of microbiology 2022-09, Vol.53 (3), p.1491-1499 |
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| creator | Debnath, Anusuya Miyoshi, Shin-Ichi |
| description | Vibrio parahaemolyticus
can degrade insoluble chitin with the help of chitinase enzymes that generate soluble N-acetyl glucosamine oligosaccharides (GlcNAc
n
) to induce a state of natural competence for the uptake of extracellular DNA. In this study, we had evaluated the role of various regulatory factors such as TfoX, CytR, OpaR, and RpoS during natural transformation of
V. parahaemolyticus
. The results suggest that TfoX regulates natural competence via CytR in a chitin-dependent manner. CytR controls the release of GlcNAc
6
from insoluble chitin and conversion of GlcNAc
6
into smaller GlcNAc residues inside the periplasm by modulating the expression of endochitinase and periplasmic chitinases. In addition, CytR was also responsible for GlcNAc
6
-mediated upregulation of competence-related genes such as
pilA
,
pilB
,
comEA
, and
qstR
. Next, we found that the quorum sensing regulator OpaR affects the natural transformation through its regulation of extracellular nuclease Dns. The Δ
opaR
mutant showed increased expression of Dns, which might degrade the eDNA. As a consequence, the transformation efficiency was decreased and eDNA-dependent growth was hugely enhanced. However, when Dns-containing DASW was substituted with fresh DASW, the transformation was detectable in Δ
opaR
mutant and eDNA-dependent growth was less. These results suggest that the occurrence of natural transformation and eDNA-dependent growth were inversely related to each other. Lastly, the general stress regulator RpoS was required for neither quorum-sensing dependent nor chitin-dependent regulation of natural competence in
V. parahaemolyticus
. |
| doi_str_mv | 10.1007/s42770-022-00788-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9433500</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2681813902</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-b044f34113ce28d81dc1d14e4d3779f7442d9ccbea00e031d0af4f9a9b5596ce3</originalsourceid><addsrcrecordid>eNp9kUtLxTAQhYMovv-ACym4cVOdPNqkCIKILxAEUbchTafXSttck1a4_97ovV4fC1eZMN-cnMkhZI_CEQWQx0EwKSEFxtJ4VSqFFbJJc6lSISBbjXVGZaq4YhtkK4QXAJaBYOtkg2cyjxLFJjm5x8nYmsH5kLg66c0wetMm1nVTHLC3mDR98tSUvnHJ1HjzbLBz7Wxo7Bh2yFpt2oC7i3ObPF5ePJxfp7d3VzfnZ7epFVIMaQlC1FxQyi0yVSlaWVpRgaLiUha1FIJVhbUlGgAETiswtagLU5RZVuQW-TY5netOx7LDymI_RI966pvO-Jl2ptG_O33zrCfuTReC8wwgChwuBLx7HTEMumuCxbY1PboxaJYrqigvgEX04A_64kbfx_U0k6BkHn9WRorNKetdCB7rpRkK-iMbPc9Gx2z0Zzb6w8X-zzWWI19hRIDPgRBb_QT999v_yL4DYbmakQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2708764057</pqid></control><display><type>article</type><title>Regulators of natural competence in Vibrio parahaemolyticus</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>SpringerLink Journals - AutoHoldings</source><creator>Debnath, Anusuya ; Miyoshi, Shin-Ichi</creator><creatorcontrib>Debnath, Anusuya ; Miyoshi, Shin-Ichi</creatorcontrib><description>Vibrio parahaemolyticus
can degrade insoluble chitin with the help of chitinase enzymes that generate soluble N-acetyl glucosamine oligosaccharides (GlcNAc
n
) to induce a state of natural competence for the uptake of extracellular DNA. In this study, we had evaluated the role of various regulatory factors such as TfoX, CytR, OpaR, and RpoS during natural transformation of
V. parahaemolyticus
. The results suggest that TfoX regulates natural competence via CytR in a chitin-dependent manner. CytR controls the release of GlcNAc
6
from insoluble chitin and conversion of GlcNAc
6
into smaller GlcNAc residues inside the periplasm by modulating the expression of endochitinase and periplasmic chitinases. In addition, CytR was also responsible for GlcNAc
6
-mediated upregulation of competence-related genes such as
pilA
,
pilB
,
comEA
, and
qstR
. Next, we found that the quorum sensing regulator OpaR affects the natural transformation through its regulation of extracellular nuclease Dns. The Δ
opaR
mutant showed increased expression of Dns, which might degrade the eDNA. As a consequence, the transformation efficiency was decreased and eDNA-dependent growth was hugely enhanced. However, when Dns-containing DASW was substituted with fresh DASW, the transformation was detectable in Δ
opaR
mutant and eDNA-dependent growth was less. These results suggest that the occurrence of natural transformation and eDNA-dependent growth were inversely related to each other. Lastly, the general stress regulator RpoS was required for neither quorum-sensing dependent nor chitin-dependent regulation of natural competence in
V. parahaemolyticus
.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-022-00788-0</identifier><identifier>PMID: 35761009</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biomedical and Life Sciences ; Chitin ; Chitin - metabolism ; Chitinase ; Chitinases - genetics ; Chitinases - metabolism ; Endochitinase ; Environmental DNA ; Environmental Microbiology - Research Paper ; Food Microbiology ; Gene Expression Regulation, Bacterial ; Genetic transformation ; Glucosamine ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mutants ; Mycology ; Nuclease ; Oligosaccharides ; Periplasm ; PilA protein ; Quorum sensing ; Transformations ; Vibrio cholerae - genetics ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - genetics ; Vibrio parahaemolyticus - metabolism</subject><ispartof>Brazilian journal of microbiology, 2022-09, Vol.53 (3), p.1491-1499</ispartof><rights>The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2022</rights><rights>2022. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.</rights><rights>The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-b044f34113ce28d81dc1d14e4d3779f7442d9ccbea00e031d0af4f9a9b5596ce3</citedby><cites>FETCH-LOGICAL-c474t-b044f34113ce28d81dc1d14e4d3779f7442d9ccbea00e031d0af4f9a9b5596ce3</cites><orcidid>0000-0001-7660-8715</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433500/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9433500/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,41486,42555,51317,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35761009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Debnath, Anusuya</creatorcontrib><creatorcontrib>Miyoshi, Shin-Ichi</creatorcontrib><title>Regulators of natural competence in Vibrio parahaemolyticus</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Vibrio parahaemolyticus
can degrade insoluble chitin with the help of chitinase enzymes that generate soluble N-acetyl glucosamine oligosaccharides (GlcNAc
n
) to induce a state of natural competence for the uptake of extracellular DNA. In this study, we had evaluated the role of various regulatory factors such as TfoX, CytR, OpaR, and RpoS during natural transformation of
V. parahaemolyticus
. The results suggest that TfoX regulates natural competence via CytR in a chitin-dependent manner. CytR controls the release of GlcNAc
6
from insoluble chitin and conversion of GlcNAc
6
into smaller GlcNAc residues inside the periplasm by modulating the expression of endochitinase and periplasmic chitinases. In addition, CytR was also responsible for GlcNAc
6
-mediated upregulation of competence-related genes such as
pilA
,
pilB
,
comEA
, and
qstR
. Next, we found that the quorum sensing regulator OpaR affects the natural transformation through its regulation of extracellular nuclease Dns. The Δ
opaR
mutant showed increased expression of Dns, which might degrade the eDNA. As a consequence, the transformation efficiency was decreased and eDNA-dependent growth was hugely enhanced. However, when Dns-containing DASW was substituted with fresh DASW, the transformation was detectable in Δ
opaR
mutant and eDNA-dependent growth was less. These results suggest that the occurrence of natural transformation and eDNA-dependent growth were inversely related to each other. Lastly, the general stress regulator RpoS was required for neither quorum-sensing dependent nor chitin-dependent regulation of natural competence in
V. parahaemolyticus
.</description><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Chitin</subject><subject>Chitin - metabolism</subject><subject>Chitinase</subject><subject>Chitinases - genetics</subject><subject>Chitinases - metabolism</subject><subject>Endochitinase</subject><subject>Environmental DNA</subject><subject>Environmental Microbiology - Research Paper</subject><subject>Food Microbiology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetic transformation</subject><subject>Glucosamine</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mutants</subject><subject>Mycology</subject><subject>Nuclease</subject><subject>Oligosaccharides</subject><subject>Periplasm</subject><subject>PilA protein</subject><subject>Quorum sensing</subject><subject>Transformations</subject><subject>Vibrio cholerae - genetics</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - genetics</subject><subject>Vibrio parahaemolyticus - metabolism</subject><issn>1517-8382</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtLxTAQhYMovv-ACym4cVOdPNqkCIKILxAEUbchTafXSttck1a4_97ovV4fC1eZMN-cnMkhZI_CEQWQx0EwKSEFxtJ4VSqFFbJJc6lSISBbjXVGZaq4YhtkK4QXAJaBYOtkg2cyjxLFJjm5x8nYmsH5kLg66c0wetMm1nVTHLC3mDR98tSUvnHJ1HjzbLBz7Wxo7Bh2yFpt2oC7i3ObPF5ePJxfp7d3VzfnZ7epFVIMaQlC1FxQyi0yVSlaWVpRgaLiUha1FIJVhbUlGgAETiswtagLU5RZVuQW-TY5netOx7LDymI_RI966pvO-Jl2ptG_O33zrCfuTReC8wwgChwuBLx7HTEMumuCxbY1PboxaJYrqigvgEX04A_64kbfx_U0k6BkHn9WRorNKetdCB7rpRkK-iMbPc9Gx2z0Zzb6w8X-zzWWI19hRIDPgRBb_QT999v_yL4DYbmakQ</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Debnath, Anusuya</creator><creator>Miyoshi, Shin-Ichi</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7660-8715</orcidid></search><sort><creationdate>20220901</creationdate><title>Regulators of natural competence in Vibrio parahaemolyticus</title><author>Debnath, Anusuya ; Miyoshi, Shin-Ichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-b044f34113ce28d81dc1d14e4d3779f7442d9ccbea00e031d0af4f9a9b5596ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Chitin</topic><topic>Chitin - metabolism</topic><topic>Chitinase</topic><topic>Chitinases - genetics</topic><topic>Chitinases - metabolism</topic><topic>Endochitinase</topic><topic>Environmental DNA</topic><topic>Environmental Microbiology - Research Paper</topic><topic>Food Microbiology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetic transformation</topic><topic>Glucosamine</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mutants</topic><topic>Mycology</topic><topic>Nuclease</topic><topic>Oligosaccharides</topic><topic>Periplasm</topic><topic>PilA protein</topic><topic>Quorum sensing</topic><topic>Transformations</topic><topic>Vibrio cholerae - genetics</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - genetics</topic><topic>Vibrio parahaemolyticus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Debnath, Anusuya</creatorcontrib><creatorcontrib>Miyoshi, Shin-Ichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debnath, Anusuya</au><au>Miyoshi, Shin-Ichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulators of natural competence in Vibrio parahaemolyticus</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2022-09-01</date><risdate>2022</risdate><volume>53</volume><issue>3</issue><spage>1491</spage><epage>1499</epage><pages>1491-1499</pages><issn>1517-8382</issn><eissn>1678-4405</eissn><abstract>Vibrio parahaemolyticus
can degrade insoluble chitin with the help of chitinase enzymes that generate soluble N-acetyl glucosamine oligosaccharides (GlcNAc
n
) to induce a state of natural competence for the uptake of extracellular DNA. In this study, we had evaluated the role of various regulatory factors such as TfoX, CytR, OpaR, and RpoS during natural transformation of
V. parahaemolyticus
. The results suggest that TfoX regulates natural competence via CytR in a chitin-dependent manner. CytR controls the release of GlcNAc
6
from insoluble chitin and conversion of GlcNAc
6
into smaller GlcNAc residues inside the periplasm by modulating the expression of endochitinase and periplasmic chitinases. In addition, CytR was also responsible for GlcNAc
6
-mediated upregulation of competence-related genes such as
pilA
,
pilB
,
comEA
, and
qstR
. Next, we found that the quorum sensing regulator OpaR affects the natural transformation through its regulation of extracellular nuclease Dns. The Δ
opaR
mutant showed increased expression of Dns, which might degrade the eDNA. As a consequence, the transformation efficiency was decreased and eDNA-dependent growth was hugely enhanced. However, when Dns-containing DASW was substituted with fresh DASW, the transformation was detectable in Δ
opaR
mutant and eDNA-dependent growth was less. These results suggest that the occurrence of natural transformation and eDNA-dependent growth were inversely related to each other. Lastly, the general stress regulator RpoS was required for neither quorum-sensing dependent nor chitin-dependent regulation of natural competence in
V. parahaemolyticus
.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>35761009</pmid><doi>10.1007/s42770-022-00788-0</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7660-8715</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Chitin Chitin - metabolism Chitinase Chitinases - genetics Chitinases - metabolism Endochitinase Environmental DNA Environmental Microbiology - Research Paper Food Microbiology Gene Expression Regulation, Bacterial Genetic transformation Glucosamine Life Sciences Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mutants Mycology Nuclease Oligosaccharides Periplasm PilA protein Quorum sensing Transformations Vibrio cholerae - genetics Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - metabolism |
| title | Regulators of natural competence in Vibrio parahaemolyticus |
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