Implantable Aptamer-Graphene Microtransistors for Real-Time Monitoring of Neurochemical Release in Vivo
The real-time monitoring of neurochemical release in vivo plays a critical role in understanding the biochemical process of the complex nervous system. Current technologies for such applications, including microdialysis and fast-scan cyclic voltammetry, suffer from limited spatiotemporal resolution...
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| Veröffentlicht in: | Nano letters 2022-05, Vol.22 (9), p.3668-3677 |
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| creator | Wu, Guangfu Zhang, Nannan Matarasso, Avi Heck, Ian Li, Huijie Lu, Wei Phaup, J. Glenn Schneider, Michael J. Wu, Yixin Weng, Zhengyan Sun, He Gao, Zan Zhang, Xincheng Sandberg, Stefan G. Parvin, Dilruba Seaholm, Elena Islam, Syed Kamrul Wang, Xueju Phillips, Paul E. M. Castro, Daniel C. Ding, Shinghua Li, De-Pei Bruchas, Michael R. Zhang, Yi |
| description | The real-time monitoring of neurochemical release in vivo plays a critical role in understanding the biochemical process of the complex nervous system. Current technologies for such applications, including microdialysis and fast-scan cyclic voltammetry, suffer from limited spatiotemporal resolution or poor selectivity. Here, we report a soft implantable aptamer-graphene microtransistor probe for real-time monitoring of neurochemical release. As a demonstration, we show the monitoring of dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), and picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex vivo experiments, and in vivo studies in mice models highlight the key features and demonstrate the capability of capturing the dopamine release dynamics evoked by pharmacological stimulation, suggesting the potential applications in basic neuroscience studies and studying neurological disease-related processes. The developed system can be easily adapted for monitoring other neurochemicals and drugs by simply replacing the aptamers functionalized on the graphene microtransistors. |
| doi_str_mv | 10.1021/acs.nanolett.2c00289 |
| format | Article |
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Glenn ; Schneider, Michael J. ; Wu, Yixin ; Weng, Zhengyan ; Sun, He ; Gao, Zan ; Zhang, Xincheng ; Sandberg, Stefan G. ; Parvin, Dilruba ; Seaholm, Elena ; Islam, Syed Kamrul ; Wang, Xueju ; Phillips, Paul E. M. ; Castro, Daniel C. ; Ding, Shinghua ; Li, De-Pei ; Bruchas, Michael R. ; Zhang, Yi</creator><creatorcontrib>Wu, Guangfu ; Zhang, Nannan ; Matarasso, Avi ; Heck, Ian ; Li, Huijie ; Lu, Wei ; Phaup, J. Glenn ; Schneider, Michael J. ; Wu, Yixin ; Weng, Zhengyan ; Sun, He ; Gao, Zan ; Zhang, Xincheng ; Sandberg, Stefan G. ; Parvin, Dilruba ; Seaholm, Elena ; Islam, Syed Kamrul ; Wang, Xueju ; Phillips, Paul E. M. ; Castro, Daniel C. ; Ding, Shinghua ; Li, De-Pei ; Bruchas, Michael R. ; Zhang, Yi</creatorcontrib><description>The real-time monitoring of neurochemical release in vivo plays a critical role in understanding the biochemical process of the complex nervous system. Current technologies for such applications, including microdialysis and fast-scan cyclic voltammetry, suffer from limited spatiotemporal resolution or poor selectivity. Here, we report a soft implantable aptamer-graphene microtransistor probe for real-time monitoring of neurochemical release. As a demonstration, we show the monitoring of dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), and picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex vivo experiments, and in vivo studies in mice models highlight the key features and demonstrate the capability of capturing the dopamine release dynamics evoked by pharmacological stimulation, suggesting the potential applications in basic neuroscience studies and studying neurological disease-related processes. The developed system can be easily adapted for monitoring other neurochemicals and drugs by simply replacing the aptamers functionalized on the graphene microtransistors.</description><identifier>ISSN: 1530-6984</identifier><identifier>ISSN: 1530-6992</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/acs.nanolett.2c00289</identifier><identifier>PMID: 35439419</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Dopamine ; Graphite ; Mice ; Norepinephrine ; Oligonucleotides</subject><ispartof>Nano letters, 2022-05, Vol.22 (9), p.3668-3677</ispartof><rights>2022 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a3649-df3f56c719a17dec608b28e8155848be9e1f123b7a4aa298b01d9b20148dca203</citedby><cites>FETCH-LOGICAL-a3649-df3f56c719a17dec608b28e8155848be9e1f123b7a4aa298b01d9b20148dca203</cites><orcidid>0000-0002-0669-8759 ; 0000-0002-0907-663X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.2c00289$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.nanolett.2c00289$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35439419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Guangfu</creatorcontrib><creatorcontrib>Zhang, Nannan</creatorcontrib><creatorcontrib>Matarasso, Avi</creatorcontrib><creatorcontrib>Heck, Ian</creatorcontrib><creatorcontrib>Li, Huijie</creatorcontrib><creatorcontrib>Lu, Wei</creatorcontrib><creatorcontrib>Phaup, J. Glenn</creatorcontrib><creatorcontrib>Schneider, Michael J.</creatorcontrib><creatorcontrib>Wu, Yixin</creatorcontrib><creatorcontrib>Weng, Zhengyan</creatorcontrib><creatorcontrib>Sun, He</creatorcontrib><creatorcontrib>Gao, Zan</creatorcontrib><creatorcontrib>Zhang, Xincheng</creatorcontrib><creatorcontrib>Sandberg, Stefan G.</creatorcontrib><creatorcontrib>Parvin, Dilruba</creatorcontrib><creatorcontrib>Seaholm, Elena</creatorcontrib><creatorcontrib>Islam, Syed Kamrul</creatorcontrib><creatorcontrib>Wang, Xueju</creatorcontrib><creatorcontrib>Phillips, Paul E. 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As a demonstration, we show the monitoring of dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), and picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex vivo experiments, and in vivo studies in mice models highlight the key features and demonstrate the capability of capturing the dopamine release dynamics evoked by pharmacological stimulation, suggesting the potential applications in basic neuroscience studies and studying neurological disease-related processes. The developed system can be easily adapted for monitoring other neurochemicals and drugs by simply replacing the aptamers functionalized on the graphene microtransistors.</description><subject>Animals</subject><subject>Dopamine</subject><subject>Graphite</subject><subject>Mice</subject><subject>Norepinephrine</subject><subject>Oligonucleotides</subject><issn>1530-6984</issn><issn>1530-6992</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQtRCIlsI_QChHLln8lax9QaqqUiq1RapartbEmey6cuxgJ5X493i12xVcOM1o5r03o_cI-cjoilHOvoDNqwAhepznFbeUcqVfkVPWCFq3WvPXx17JE_Iu5ydKqRYNfUtORCOFlkyfks31OHkIM3Qeq_NphhFTfZVg2mLA6tbZFOcEIbs8x5SrIabqHsHXD24s6xhcGbuwqeJQ3eGSot3i6Cz4gvIIGSsXqp_uOb4nbwbwGT8c6hl5_Hb5cPG9vvlxdX1xflODaKWu-0EMTWvXTANb92hbqjquULGmUVJ1qJENjItuDRKAa9VR1uuOUyZVb4FTcUa-7nWnpRuxtxjK-95MyY2QfpsIzvy7CW5rNvHZaFnYQhaBzweBFH8tmGczumzRF5MwLtnwtuGqlWu9uyX30GJSzgmH4xlGzS4jUzIyLxmZQ0aF9unvF4-kl1AKgO4BO_pTXFIojv1f8w_TXaPy</recordid><startdate>20220511</startdate><enddate>20220511</enddate><creator>Wu, Guangfu</creator><creator>Zhang, Nannan</creator><creator>Matarasso, Avi</creator><creator>Heck, Ian</creator><creator>Li, Huijie</creator><creator>Lu, Wei</creator><creator>Phaup, J. 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M.</creator><creator>Castro, Daniel C.</creator><creator>Ding, Shinghua</creator><creator>Li, De-Pei</creator><creator>Bruchas, Michael R.</creator><creator>Zhang, Yi</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0669-8759</orcidid><orcidid>https://orcid.org/0000-0002-0907-663X</orcidid></search><sort><creationdate>20220511</creationdate><title>Implantable Aptamer-Graphene Microtransistors for Real-Time Monitoring of Neurochemical Release in Vivo</title><author>Wu, Guangfu ; Zhang, Nannan ; Matarasso, Avi ; Heck, Ian ; Li, Huijie ; Lu, Wei ; Phaup, J. Glenn ; Schneider, Michael J. ; Wu, Yixin ; Weng, Zhengyan ; Sun, He ; Gao, Zan ; Zhang, Xincheng ; Sandberg, Stefan G. ; Parvin, Dilruba ; Seaholm, Elena ; Islam, Syed Kamrul ; Wang, Xueju ; Phillips, Paul E. 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Here, we report a soft implantable aptamer-graphene microtransistor probe for real-time monitoring of neurochemical release. As a demonstration, we show the monitoring of dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), and picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex vivo experiments, and in vivo studies in mice models highlight the key features and demonstrate the capability of capturing the dopamine release dynamics evoked by pharmacological stimulation, suggesting the potential applications in basic neuroscience studies and studying neurological disease-related processes. The developed system can be easily adapted for monitoring other neurochemicals and drugs by simply replacing the aptamers functionalized on the graphene microtransistors.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>35439419</pmid><doi>10.1021/acs.nanolett.2c00289</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0669-8759</orcidid><orcidid>https://orcid.org/0000-0002-0907-663X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Nano letters, 2022-05, Vol.22 (9), p.3668-3677 |
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| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Dopamine Graphite Mice Norepinephrine Oligonucleotides |
| title | Implantable Aptamer-Graphene Microtransistors for Real-Time Monitoring of Neurochemical Release in Vivo |
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