The Use of 3D Polylactic Acid Scaffolds with Hydroxyapatite/Alginate Composite Injection and Mesenchymal Stem Cells as Laminoplasty Spacers in Rabbits
Several types of laminoplasty spacer have been used to fill bone gaps and maintain a widened canal. A 3D scaffold can be used as an alternative spacer to minimize the risk observed in allografts or autografts. This study aims to evaluate the in vivo biocompatibility and tissue−scaffold integration o...
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| Veröffentlicht in: | Polymers 2022-08, Vol.14 (16), p.3292 |
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| creator | Rahyussalim, Ahmad Jabir Aprilya, Dina Handidwiono, Raden Whulanza, Yudan Ramahdita, Ghiska Kurniawati, Tri |
| description | Several types of laminoplasty spacer have been used to fill bone gaps and maintain a widened canal. A 3D scaffold can be used as an alternative spacer to minimize the risk observed in allografts or autografts. This study aims to evaluate the in vivo biocompatibility and tissue−scaffold integration of a polylactic acid (PLA) scaffold with the addition of alginate/hydroxyapatite (HA) and mesenchymal stem cell (MSc) injections. This is an experimental study with a pretest and post-test control group design. A total of 15 laminoplasty rabbit models were divided into five groups with variations in the autograft, PLA, HA/alginate, and MSc scaffold. In general, there were no signs of inflammation in most samples (47%), and there were no samples with areas of necrosis. There were no significant differences in the histopathological results and microstructural assessment between the five groups. This demonstrates that the synthetic scaffolds that we used had a similar tissue reaction and tissue integration profile as the autograft (p > 0.05). We recommend further translational studies in humans so that this biocompatible fabricated scaffold can be used to fill bone defects. |
| doi_str_mv | 10.3390/polym14163292 |
| format | Article |
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A 3D scaffold can be used as an alternative spacer to minimize the risk observed in allografts or autografts. This study aims to evaluate the in vivo biocompatibility and tissue−scaffold integration of a polylactic acid (PLA) scaffold with the addition of alginate/hydroxyapatite (HA) and mesenchymal stem cell (MSc) injections. This is an experimental study with a pretest and post-test control group design. A total of 15 laminoplasty rabbit models were divided into five groups with variations in the autograft, PLA, HA/alginate, and MSc scaffold. In general, there were no signs of inflammation in most samples (47%), and there were no samples with areas of necrosis. There were no significant differences in the histopathological results and microstructural assessment between the five groups. This demonstrates that the synthetic scaffolds that we used had a similar tissue reaction and tissue integration profile as the autograft (p > 0.05). We recommend further translational studies in humans so that this biocompatible fabricated scaffold can be used to fill bone defects.</description><identifier>ISSN: 2073-4360</identifier><identifier>EISSN: 2073-4360</identifier><identifier>DOI: 10.3390/polym14163292</identifier><identifier>PMID: 36015548</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Alginates ; Analysis ; Biocompatibility ; Biomedical materials ; Biopolymers ; Cell growth ; Disease transmission ; Hydroxyapatite ; In vivo methods and tests ; Mechanical properties ; Necrosis ; Performance evaluation ; Polylactic acid ; Polymers ; Rabbits ; Scaffolds ; Sodium ; Software ; Spinal cord ; Stem cells ; Tissue engineering ; Transplantation</subject><ispartof>Polymers, 2022-08, Vol.14 (16), p.3292</ispartof><rights>COPYRIGHT 2022 MDPI AG</rights><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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We recommend further translational studies in humans so that this biocompatible fabricated scaffold can be used to fill bone defects.</description><subject>Alginates</subject><subject>Analysis</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Biopolymers</subject><subject>Cell growth</subject><subject>Disease transmission</subject><subject>Hydroxyapatite</subject><subject>In vivo methods and tests</subject><subject>Mechanical properties</subject><subject>Necrosis</subject><subject>Performance evaluation</subject><subject>Polylactic acid</subject><subject>Polymers</subject><subject>Rabbits</subject><subject>Scaffolds</subject><subject>Sodium</subject><subject>Software</subject><subject>Spinal cord</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Transplantation</subject><issn>2073-4360</issn><issn>2073-4360</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkk1vEzEQhlcIRKvSI1dkiQuXtP5a7_qCFKWUVgoCkXC2vP5IHHntZe0A-0f6e3HUUhqEffBo_LyvNeOpqtcIXhDC4eUQ_dQjihjBHD-rTjFsyIwSBp8_iU-q85R2sCxaM4aal9VJyaK6pu1pdbfeGvAtGRAtIFfgSzH0UmWnwFw5DVZKWhu9TuCny1twM-kx_prkILPL5nLuNy7IbMAi9kNMJQVuw84UeQxABg0-mWSC2k699GCVTQ8WxvsEZAJL2bsQBy9TnsBqkMqMCbgAvsquczm9ql5Y6ZM5fzjPqvX1h_XiZrb8_PF2MV_OFG1xnmlicNsybS1VlHOFGalbZCnjWneowwZbzgjiqIAd5FbDumOcU64g0m1Dzqr397bDvuuNVibkUXoxjK6X4ySidOL4Jrit2MQfgpee1w0qBu8eDMb4fW9SFr1LqhQpg4n7JHADG4Zg-Z2Cvv0H3cX9GEp1B4rhhtEa_qU20hvhgo3lXXUwFfOGMkpahnihLv5Dla1N71QMxrqSPxLM7gVqjCmNxj7WiKA4jJI4GqXCv3namEf6z-CQ3-fKxYs</recordid><startdate>20220812</startdate><enddate>20220812</enddate><creator>Rahyussalim, Ahmad Jabir</creator><creator>Aprilya, Dina</creator><creator>Handidwiono, Raden</creator><creator>Whulanza, Yudan</creator><creator>Ramahdita, Ghiska</creator><creator>Kurniawati, Tri</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6986-4833</orcidid><orcidid>https://orcid.org/0000-0003-3248-1147</orcidid><orcidid>https://orcid.org/0000-0002-1541-2118</orcidid></search><sort><creationdate>20220812</creationdate><title>The Use of 3D Polylactic Acid Scaffolds with Hydroxyapatite/Alginate Composite Injection and Mesenchymal Stem Cells as Laminoplasty Spacers in Rabbits</title><author>Rahyussalim, Ahmad Jabir ; Aprilya, Dina ; Handidwiono, Raden ; Whulanza, Yudan ; Ramahdita, Ghiska ; Kurniawati, Tri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-d3e2886dff4c499c263581f469ddb1b2e2f963191d3eb09fd05b69949c01d873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Alginates</topic><topic>Analysis</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Biopolymers</topic><topic>Cell growth</topic><topic>Disease transmission</topic><topic>Hydroxyapatite</topic><topic>In vivo methods and tests</topic><topic>Mechanical properties</topic><topic>Necrosis</topic><topic>Performance evaluation</topic><topic>Polylactic acid</topic><topic>Polymers</topic><topic>Rabbits</topic><topic>Scaffolds</topic><topic>Sodium</topic><topic>Software</topic><topic>Spinal cord</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahyussalim, Ahmad Jabir</creatorcontrib><creatorcontrib>Aprilya, Dina</creatorcontrib><creatorcontrib>Handidwiono, Raden</creatorcontrib><creatorcontrib>Whulanza, Yudan</creatorcontrib><creatorcontrib>Ramahdita, Ghiska</creatorcontrib><creatorcontrib>Kurniawati, Tri</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahyussalim, Ahmad Jabir</au><au>Aprilya, Dina</au><au>Handidwiono, Raden</au><au>Whulanza, Yudan</au><au>Ramahdita, Ghiska</au><au>Kurniawati, Tri</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Use of 3D Polylactic Acid Scaffolds with Hydroxyapatite/Alginate Composite Injection and Mesenchymal Stem Cells as Laminoplasty Spacers in Rabbits</atitle><jtitle>Polymers</jtitle><addtitle>Polymers (Basel)</addtitle><date>2022-08-12</date><risdate>2022</risdate><volume>14</volume><issue>16</issue><spage>3292</spage><pages>3292-</pages><issn>2073-4360</issn><eissn>2073-4360</eissn><abstract>Several types of laminoplasty spacer have been used to fill bone gaps and maintain a widened canal. 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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Alginates Analysis Biocompatibility Biomedical materials Biopolymers Cell growth Disease transmission Hydroxyapatite In vivo methods and tests Mechanical properties Necrosis Performance evaluation Polylactic acid Polymers Rabbits Scaffolds Sodium Software Spinal cord Stem cells Tissue engineering Transplantation |
| title | The Use of 3D Polylactic Acid Scaffolds with Hydroxyapatite/Alginate Composite Injection and Mesenchymal Stem Cells as Laminoplasty Spacers in Rabbits |
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