Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues
Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of ma...
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| Veröffentlicht in: | International journal of molecular sciences 2022-08, Vol.23 (16), p.8868 |
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| creator | Kobayashi, Mako Ishida, Naoki Hashimoto, Yoshihide Negishi, Jun Saga, Hideki Sasaki, Yoshihiro Akiyoshi, Kazunari Kimura, Tsuyoshi Kishida, Akio |
| description | Decellularized tissues are widely used as promising materials in tissue engineering and regenerative medicine. Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling. |
| doi_str_mv | 10.3390/ijms23168868 |
| format | Article |
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Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms23168868</identifier><identifier>PMID: 36012126</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Biocompatibility ; Biological activity ; Cell growth ; Cell proliferation ; Endothelial cells ; Extracellular matrix ; Growth factors ; Hydrostatic pressure ; In vivo methods and tests ; Liver ; Methods ; Nanoparticles ; Particle size ; Regeneration (physiology) ; Regenerative medicine ; Surfactants ; Tissue engineering ; Transmission electron microscopy ; Urinary bladder</subject><ispartof>International journal of molecular sciences, 2022-08, Vol.23 (16), p.8868</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.</description><subject>Biocompatibility</subject><subject>Biological activity</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Endothelial cells</subject><subject>Extracellular matrix</subject><subject>Growth factors</subject><subject>Hydrostatic pressure</subject><subject>In vivo methods and tests</subject><subject>Liver</subject><subject>Methods</subject><subject>Nanoparticles</subject><subject>Particle size</subject><subject>Regeneration (physiology)</subject><subject>Regenerative medicine</subject><subject>Surfactants</subject><subject>Tissue engineering</subject><subject>Transmission electron microscopy</subject><subject>Urinary bladder</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1P3DAQhi3UCijl1h9gqRcqNcVfsZNLpS5dWCRoewCuluNMFq-cGOxkBT30t9cLCAHSSDPyPPNqXg9Cnyj5xnlNDt2qT4xTWVWy2kK7VDBWECLVuxf1DvqQ0ooQxllZb6MdLglllMld9G9-N0ZjRxcGbIYWz1zwYems8Xi-Nn4yD53Q4XMzRndXzMKUqV9mCGtIznpI-OB8dpW-4C6GHi_c8rpY3LcxpDGPWvwnQkpThOInWPB-8ia6v9DiC5efIX1E7zvjE-w_5T10eTy_OFoUZ79PTo9-nBVWlOVYNAqYUATMQ0BbQwfGliAZaXglDaWmVKKTQFhTs5I1QrQgCG87SSTllO-h74-6N1PTQ2thyK69vomuN_FeB-P0687grvUyrHUtiKqYygIHTwIx3ObFR927tHFkBghT0kwRJUlVC5bRz2_QVZjikO1tKMmYqkuRqa-PlM1_lSJ0z8tQojeH1S8Py_8D6vuXkQ</recordid><startdate>20220809</startdate><enddate>20220809</enddate><creator>Kobayashi, Mako</creator><creator>Ishida, Naoki</creator><creator>Hashimoto, Yoshihide</creator><creator>Negishi, Jun</creator><creator>Saga, Hideki</creator><creator>Sasaki, Yoshihiro</creator><creator>Akiyoshi, Kazunari</creator><creator>Kimura, Tsuyoshi</creator><creator>Kishida, Akio</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0537-8291</orcidid><orcidid>https://orcid.org/0000-0003-0476-0624</orcidid><orcidid>https://orcid.org/0000-0002-2434-3564</orcidid><orcidid>https://orcid.org/0000-0002-2413-5810</orcidid></search><sort><creationdate>20220809</creationdate><title>Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues</title><author>Kobayashi, Mako ; 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Research on the microstructure and components of the extracellular matrix (ECM) was conducted to improve the current understanding of decellularized tissue functionality. The presence of matrix-bound nanovesicles (MBVs) embedded within the ECM was recently reported. Results of a previous experimental investigation revealed that decellularized tissues prepared using high hydrostatic pressure (HHP) exhibited good in vivo performance. In the current study, according to the hypothesis that MBVs are one of the functional components in HHP-decellularized tissue, we investigated the extraction of MBVs and the associated effects on vascular endothelial cells. Using nanoparticle tracking assay (NTA), transmission electron microscopy (TEM), and RNA analysis, nanosized (100–300 nm) and membranous particles containing small RNA were detected in MBVs derived from HHP-decellularized small intestinal submucosa (SIS), urinary bladder matrix (UBM), and liver. To evaluate the effect on the growth of vascular endothelial cells, which are important in the tissue regeneration process, isolated SIS-derived MBVs were exposed to vascular endothelial cells to induce cell proliferation. These results indicate that MBVs can be extracted from HHP-decellularized tissues and may play a significant role in tissue remodeling.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>36012126</pmid><doi>10.3390/ijms23168868</doi><orcidid>https://orcid.org/0000-0003-0537-8291</orcidid><orcidid>https://orcid.org/0000-0003-0476-0624</orcidid><orcidid>https://orcid.org/0000-0002-2434-3564</orcidid><orcidid>https://orcid.org/0000-0002-2413-5810</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biocompatibility Biological activity Cell growth Cell proliferation Endothelial cells Extracellular matrix Growth factors Hydrostatic pressure In vivo methods and tests Liver Methods Nanoparticles Particle size Regeneration (physiology) Regenerative medicine Surfactants Tissue engineering Transmission electron microscopy Urinary bladder |
| title | Extraction and Biological Evaluation of Matrix-Bound Nanovesicles (MBVs) from High-Hydrostatic Pressure-Decellularized Tissues |
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