Combining CRISPR–Cas12a with terminal deoxynucleotidyl transferase dependent reporter elongation for pathogen detection using lateral flow test strips

Abstract CRISPR–Cas (CC)-based detection technologies have some exceptional features, which hold the promise of developing into the next-generation diagnostic platforms. One of these features is the ability to trigger non-specific single-stranded DNA/RNA cleavage activity after specific target recog...

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Veröffentlicht in:	Biology Methods & Protocols 2022, Vol.7 (1), p.1-bpac015
Hauptverfasser:	Berghuis, Nicole F, Mars-Groenendijk, Roos, Busker, Ruud W, Paauw, Armand, van Leeuwen, Hans C
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA polymerases Enzymes Ethylenediaminetetraacetic acid Fluorescein Journalists Methods Nucleotides Pyrimidines
Online-Zugang:	Volltext
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