Development of in vivo HDX‐MS with applications to a TonB‐dependent transporter and other proteins

Hydrogen‐deuterium exchange mass spectrometry (HDX‐MS) is a powerful tool that monitors protein dynamics in solution. However, the reversible nature of HDX labels has largely limited the application to in vitro systems. Here, we describe a protocol for measuring HDX‐MS in living Escherichia coli cel...

Ausführliche Beschreibung