Temporal analysis of Lassa virus infection and transmission in experimentally infected Mastomys natalensis
Little is known about the temporal patterns of infection and transmission of Lassa virus (LASV) within its natural reservoir ( ). Here, we characterize infection dynamics and transmissibility of a LASV isolate (Soromba-R) in adult lab-reared originating from Mali. The lab-reared M. proved to be high...
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| Veröffentlicht in: | PNAS nexus 2022-07, Vol.1 (3), p.pgac114-pgac114 |
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| creator | Safronetz, David Rosenke, Kyle Meade-White, Kimberley Sloan, Angela Maiga, Ousmane Bane, Sidy Martellaro, Cynthia Scott, Dana P Sogoba, Nafomon Feldmann, Heinz |
| description | Little is known about the temporal patterns of infection and transmission of Lassa virus (LASV) within its natural reservoir (
). Here, we characterize infection dynamics and transmissibility of a LASV isolate (Soromba-R) in adult lab-reared
originating from Mali. The lab-reared M.
proved to be highly susceptible to LASV isolates from geographically distinct regions of West Africa via multiple routes of exposure, with 50% infectious doses of |
| doi_str_mv | 10.1093/pnasnexus/pgac114 |
| format | Article |
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). Here, we characterize infection dynamics and transmissibility of a LASV isolate (Soromba-R) in adult lab-reared
originating from Mali. The lab-reared M.
proved to be highly susceptible to LASV isolates from geographically distinct regions of West Africa via multiple routes of exposure, with 50% infectious doses of < 1 TCID
. Postinoculation, LASV Soromba-R established a systemic infection with no signs of clinical disease. Viral RNA was detected in all nine tissues examined with peak concentrations detected between days 7 and 14 postinfection within most organs. There was an overall trend toward clearance of virus within 40 days of infection in most organs. The exception is lung specimens, which retained positivity throughout the course of the 85-day study. Direct (contact) and indirect (fomite) transmission experiments demonstrated 40% of experimentally infected
were capable of transmitting LASV to naïve animals, with peak transmissibility occurring between 28 and 42 days post-inoculation. No differences in patterns of infection or transmission were noted between male and female experimentally infected rodents. Adult lab-reared
are highly susceptible to genetically distinct LASV strains developing a temporary asymptomatic infection associated with virus shedding resulting in contact and fomite transmission within a cohort.</description><identifier>ISSN: 2752-6542</identifier><identifier>EISSN: 2752-6542</identifier><identifier>DOI: 10.1093/pnasnexus/pgac114</identifier><identifier>PMID: 35967978</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Biological, Health, and Medical Sciences ; Development and progression ; Distribution ; Genetic aspects ; Health aspects ; Lassa fever ; Muridae</subject><ispartof>PNAS nexus, 2022-07, Vol.1 (3), p.pgac114-pgac114</ispartof><rights>Published by Oxford University Press on behalf of National Academy of Sciences 2022.</rights><rights>COPYRIGHT 2022 Oxford University Press</rights><rights>Published by Oxford University Press on behalf of National Academy of Sciences 2022. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-d28caaa1cff2e67ffd0ef7a24b9a4cdc14775d7318d174b320f71048faef435a3</citedby><cites>FETCH-LOGICAL-c466t-d28caaa1cff2e67ffd0ef7a24b9a4cdc14775d7318d174b320f71048faef435a3</cites><orcidid>0000-0002-9024-728X ; 0000-0001-8838-5803 ; 0000-0001-9448-8227</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364215/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364215/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35967978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nelson, Karen E</contributor><creatorcontrib>Safronetz, David</creatorcontrib><creatorcontrib>Rosenke, Kyle</creatorcontrib><creatorcontrib>Meade-White, Kimberley</creatorcontrib><creatorcontrib>Sloan, Angela</creatorcontrib><creatorcontrib>Maiga, Ousmane</creatorcontrib><creatorcontrib>Bane, Sidy</creatorcontrib><creatorcontrib>Martellaro, Cynthia</creatorcontrib><creatorcontrib>Scott, Dana P</creatorcontrib><creatorcontrib>Sogoba, Nafomon</creatorcontrib><creatorcontrib>Feldmann, Heinz</creatorcontrib><title>Temporal analysis of Lassa virus infection and transmission in experimentally infected Mastomys natalensis</title><title>PNAS nexus</title><addtitle>PNAS Nexus</addtitle><description>Little is known about the temporal patterns of infection and transmission of Lassa virus (LASV) within its natural reservoir (
). Here, we characterize infection dynamics and transmissibility of a LASV isolate (Soromba-R) in adult lab-reared
originating from Mali. The lab-reared M.
proved to be highly susceptible to LASV isolates from geographically distinct regions of West Africa via multiple routes of exposure, with 50% infectious doses of < 1 TCID
. Postinoculation, LASV Soromba-R established a systemic infection with no signs of clinical disease. Viral RNA was detected in all nine tissues examined with peak concentrations detected between days 7 and 14 postinfection within most organs. There was an overall trend toward clearance of virus within 40 days of infection in most organs. The exception is lung specimens, which retained positivity throughout the course of the 85-day study. Direct (contact) and indirect (fomite) transmission experiments demonstrated 40% of experimentally infected
were capable of transmitting LASV to naïve animals, with peak transmissibility occurring between 28 and 42 days post-inoculation. No differences in patterns of infection or transmission were noted between male and female experimentally infected rodents. Adult lab-reared
are highly susceptible to genetically distinct LASV strains developing a temporary asymptomatic infection associated with virus shedding resulting in contact and fomite transmission within a cohort.</description><subject>Biological, Health, and Medical Sciences</subject><subject>Development and progression</subject><subject>Distribution</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Lassa fever</subject><subject>Muridae</subject><issn>2752-6542</issn><issn>2752-6542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNptkUtr3DAUhUVpaUKaH9BNEXTTzSR62bI3hRDSB0zpJl2LO_LVVMGWXF87ZP59Ncx0SKBoIXH1ncM9HMbeS3ElRauvxwSU8Gmh63ELXkrzip0rW6lVXRn1-tn7jF0SPQghlLUFq96yM121tW1tc84e7nEY8wQ9hwT9jiLxHPgaiIA_xmkhHlNAP8ecCtHxeYJEQyTaD2Li-DTiFAdMM_T97ghjx38AzXnYEU9QfjAV43fsTYCe8PJ4X7BfX-7ub7-t1j-_fr-9Wa-8qet51anGA4D0ISisbQidwGBBmU0LxndeGmurzmrZdNKajVYiWClMEwCD0RXoC_b54DsumwE7X3Yr-dxY1oRp5zJE9_Inxd9umx9dq2ujZFUMPh0NpvxnQZpdCeyx7yFhXsgpK5RpbFOJgn48oNsS0pX0uTj6Pe5urG2kbmprCnX1H6qcDofoc8IQy_yFQB4EfspEE4bT9lK4ffvu1L47tl80H57HPin-da3_AizwsoY</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Safronetz, David</creator><creator>Rosenke, Kyle</creator><creator>Meade-White, Kimberley</creator><creator>Sloan, Angela</creator><creator>Maiga, Ousmane</creator><creator>Bane, Sidy</creator><creator>Martellaro, Cynthia</creator><creator>Scott, Dana P</creator><creator>Sogoba, Nafomon</creator><creator>Feldmann, Heinz</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9024-728X</orcidid><orcidid>https://orcid.org/0000-0001-8838-5803</orcidid><orcidid>https://orcid.org/0000-0001-9448-8227</orcidid></search><sort><creationdate>20220701</creationdate><title>Temporal analysis of Lassa virus infection and transmission in experimentally infected Mastomys natalensis</title><author>Safronetz, David ; Rosenke, Kyle ; Meade-White, Kimberley ; Sloan, Angela ; Maiga, Ousmane ; Bane, Sidy ; Martellaro, Cynthia ; Scott, Dana P ; Sogoba, Nafomon ; Feldmann, Heinz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-d28caaa1cff2e67ffd0ef7a24b9a4cdc14775d7318d174b320f71048faef435a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological, Health, and Medical Sciences</topic><topic>Development and progression</topic><topic>Distribution</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Lassa fever</topic><topic>Muridae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Safronetz, David</creatorcontrib><creatorcontrib>Rosenke, Kyle</creatorcontrib><creatorcontrib>Meade-White, Kimberley</creatorcontrib><creatorcontrib>Sloan, Angela</creatorcontrib><creatorcontrib>Maiga, Ousmane</creatorcontrib><creatorcontrib>Bane, Sidy</creatorcontrib><creatorcontrib>Martellaro, Cynthia</creatorcontrib><creatorcontrib>Scott, Dana P</creatorcontrib><creatorcontrib>Sogoba, Nafomon</creatorcontrib><creatorcontrib>Feldmann, Heinz</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>PNAS nexus</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Safronetz, David</au><au>Rosenke, Kyle</au><au>Meade-White, Kimberley</au><au>Sloan, Angela</au><au>Maiga, Ousmane</au><au>Bane, Sidy</au><au>Martellaro, Cynthia</au><au>Scott, Dana P</au><au>Sogoba, Nafomon</au><au>Feldmann, Heinz</au><au>Nelson, Karen E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal analysis of Lassa virus infection and transmission in experimentally infected Mastomys natalensis</atitle><jtitle>PNAS nexus</jtitle><addtitle>PNAS Nexus</addtitle><date>2022-07-01</date><risdate>2022</risdate><volume>1</volume><issue>3</issue><spage>pgac114</spage><epage>pgac114</epage><pages>pgac114-pgac114</pages><issn>2752-6542</issn><eissn>2752-6542</eissn><abstract>Little is known about the temporal patterns of infection and transmission of Lassa virus (LASV) within its natural reservoir (
). Here, we characterize infection dynamics and transmissibility of a LASV isolate (Soromba-R) in adult lab-reared
originating from Mali. The lab-reared M.
proved to be highly susceptible to LASV isolates from geographically distinct regions of West Africa via multiple routes of exposure, with 50% infectious doses of < 1 TCID
. Postinoculation, LASV Soromba-R established a systemic infection with no signs of clinical disease. Viral RNA was detected in all nine tissues examined with peak concentrations detected between days 7 and 14 postinfection within most organs. There was an overall trend toward clearance of virus within 40 days of infection in most organs. The exception is lung specimens, which retained positivity throughout the course of the 85-day study. Direct (contact) and indirect (fomite) transmission experiments demonstrated 40% of experimentally infected
were capable of transmitting LASV to naïve animals, with peak transmissibility occurring between 28 and 42 days post-inoculation. No differences in patterns of infection or transmission were noted between male and female experimentally infected rodents. Adult lab-reared
are highly susceptible to genetically distinct LASV strains developing a temporary asymptomatic infection associated with virus shedding resulting in contact and fomite transmission within a cohort.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>35967978</pmid><doi>10.1093/pnasnexus/pgac114</doi><orcidid>https://orcid.org/0000-0002-9024-728X</orcidid><orcidid>https://orcid.org/0000-0001-8838-5803</orcidid><orcidid>https://orcid.org/0000-0001-9448-8227</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Access via Oxford University Press (Open Access Collection); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biological, Health, and Medical Sciences Development and progression Distribution Genetic aspects Health aspects Lassa fever Muridae |
| title | Temporal analysis of Lassa virus infection and transmission in experimentally infected Mastomys natalensis |
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