Ultrabright nanoparticle-labeled lateral flow immunoassay for detection of anti-SARS-CoV-2 neutralizing antibodies in human serum
The level of anti-SARS-CoV-2 neutralizing antibodies (NAb) is an indispensable reference for evaluating the acquired protective immunity against SARS-CoV-2. Here, we established an ultrabright nanoparticles-based lateral flow immunoassay (LFIA) for one-step rapid semi-quantitative detection of anti-...
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| Veröffentlicht in: | Biomaterials 2022-09, Vol.288, p.121694-121694, Article 121694 |
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| container_title | Biomaterials |
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| creator | Bian, Lun Li, Zhaoyue He, An Wu, Biru Yang, Hui Wu, Yingsong Hu, Fang Lin, Guanfeng Zhang, Deqing |
| description | The level of anti-SARS-CoV-2 neutralizing antibodies (NAb) is an indispensable reference for evaluating the acquired protective immunity against SARS-CoV-2. Here, we established an ultrabright nanoparticles-based lateral flow immunoassay (LFIA) for one-step rapid semi-quantitative detection of anti-SARS-CoV-2 NAb in vaccinee's serum. Once embedded in polystyrene (PS) nanoparticles, the aggregation-induced emission (AIE) luminogen, AIE490, exhibited ultrabright fluorescence due to the rigidity of PS and severe inhibition of intramolecular motions. The ultrabright AIE490-PS nanoparticle was used as a fluorescent marker of LFIA. Upon optimized conditions including incubation time, concentrations of coated proteins and conjugated nanoparticles, amounts of antigens modified on the surface of nanoparticles, dilution rate of serum samples, and so on, the ultrabright nanoparticles-based LFIA could accurately identify 70 negative samples and 63 positive samples from human serum (p |
| doi_str_mv | 10.1016/j.biomaterials.2022.121694 |
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Here, we established an ultrabright nanoparticles-based lateral flow immunoassay (LFIA) for one-step rapid semi-quantitative detection of anti-SARS-CoV-2 NAb in vaccinee's serum. Once embedded in polystyrene (PS) nanoparticles, the aggregation-induced emission (AIE) luminogen, AIE490, exhibited ultrabright fluorescence due to the rigidity of PS and severe inhibition of intramolecular motions. The ultrabright AIE490-PS nanoparticle was used as a fluorescent marker of LFIA. Upon optimized conditions including incubation time, concentrations of coated proteins and conjugated nanoparticles, amounts of antigens modified on the surface of nanoparticles, dilution rate of serum samples, and so on, the ultrabright nanoparticles-based LFIA could accurately identify 70 negative samples and 63 positive samples from human serum (p < 0.0001). The intra- and inter-assay precisions of the established method are above 13% and 16%, respectively. The established LFIA has tremendous practical value of generalization as a rapid semi-quantitative detection method of anti-SARS-CoV-2 NAb. Meanwhile, the AIE490-PS nanoparticle is also promising to detect many other analytes by altering the protein on the surface.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2022.121694</identifier><identifier>PMID: 35977850</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Aggregation-induced emission ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 - diagnosis ; Humans ; Immunoassay - methods ; Lateral flow immunoassay ; Nanoparticles ; Neutralizing antibody ; SARS-CoV-2</subject><ispartof>Biomaterials, 2022-09, Vol.288, p.121694-121694, Article 121694</ispartof><rights>2022 Elsevier Ltd</rights><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><rights>2022 Elsevier Ltd. All rights reserved. 2022 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c553t-ab25dbfc923d57f3146fa94400340a8125ca4358617d4d4ba7485e42c21012453</citedby><cites>FETCH-LOGICAL-c553t-ab25dbfc923d57f3146fa94400340a8125ca4358617d4d4ba7485e42c21012453</cites><orcidid>0000-0001-5180-4963 ; 0000-0002-1150-9625</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961222003349$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35977850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bian, Lun</creatorcontrib><creatorcontrib>Li, Zhaoyue</creatorcontrib><creatorcontrib>He, An</creatorcontrib><creatorcontrib>Wu, Biru</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Wu, Yingsong</creatorcontrib><creatorcontrib>Hu, Fang</creatorcontrib><creatorcontrib>Lin, Guanfeng</creatorcontrib><creatorcontrib>Zhang, Deqing</creatorcontrib><title>Ultrabright nanoparticle-labeled lateral flow immunoassay for detection of anti-SARS-CoV-2 neutralizing antibodies in human serum</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>The level of anti-SARS-CoV-2 neutralizing antibodies (NAb) is an indispensable reference for evaluating the acquired protective immunity against SARS-CoV-2. Here, we established an ultrabright nanoparticles-based lateral flow immunoassay (LFIA) for one-step rapid semi-quantitative detection of anti-SARS-CoV-2 NAb in vaccinee's serum. Once embedded in polystyrene (PS) nanoparticles, the aggregation-induced emission (AIE) luminogen, AIE490, exhibited ultrabright fluorescence due to the rigidity of PS and severe inhibition of intramolecular motions. The ultrabright AIE490-PS nanoparticle was used as a fluorescent marker of LFIA. Upon optimized conditions including incubation time, concentrations of coated proteins and conjugated nanoparticles, amounts of antigens modified on the surface of nanoparticles, dilution rate of serum samples, and so on, the ultrabright nanoparticles-based LFIA could accurately identify 70 negative samples and 63 positive samples from human serum (p < 0.0001). The intra- and inter-assay precisions of the established method are above 13% and 16%, respectively. The established LFIA has tremendous practical value of generalization as a rapid semi-quantitative detection method of anti-SARS-CoV-2 NAb. Meanwhile, the AIE490-PS nanoparticle is also promising to detect many other analytes by altering the protein on the surface.</description><subject>Aggregation-induced emission</subject><subject>Antibodies, Neutralizing</subject><subject>Antibodies, Viral</subject><subject>COVID-19 - diagnosis</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Lateral flow immunoassay</subject><subject>Nanoparticles</subject><subject>Neutralizing antibody</subject><subject>SARS-CoV-2</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuLFDEUhYMoTjv6FyS4rzZJJfVwIQw9vmBAcBy3IZXc6r5NKmmSqpFx5z83besw7lyFcO85JzkfIa84W3PGm9f79YBxMjMkND6vBRNizQVvevmIrHjXdpXqmXpMVoxLUfUNF2fkWc57Vu5MiqfkrFZ923aKrcjPGz8nMyTc7mYaTIgHk2a0HipvBvDgqD8GGU9HH79TnKYlRJOzuaNjTNTBDHbGGGgcqQkzVtcXX66rTfxWCRpgKd4ef2DY_h4O0SFkioHulskEmiEt03PyZCzfgBd_znNy8_7d183H6urzh0-bi6vKKlXPlRmEcsNoe1E71Y41l81oeikZqyUzHRfKGlmrruGtk04OppWdAimsKJ0Jqepz8vbke1iGCZyFcHycPiScTLrT0aD-dxJwp7fxVvd1w9pWFoM3JwObYs4JxnstZ_oIRu_1QzD6CEafwBTxy4fp99K_JMrC5WkBSge3CElnixAsOEylYu0i_k_OL6RLqZ4</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Bian, Lun</creator><creator>Li, Zhaoyue</creator><creator>He, An</creator><creator>Wu, Biru</creator><creator>Yang, Hui</creator><creator>Wu, Yingsong</creator><creator>Hu, Fang</creator><creator>Lin, Guanfeng</creator><creator>Zhang, Deqing</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5180-4963</orcidid><orcidid>https://orcid.org/0000-0002-1150-9625</orcidid></search><sort><creationdate>20220901</creationdate><title>Ultrabright nanoparticle-labeled lateral flow immunoassay for detection of anti-SARS-CoV-2 neutralizing antibodies in human serum</title><author>Bian, Lun ; Li, Zhaoyue ; He, An ; Wu, Biru ; Yang, Hui ; Wu, Yingsong ; Hu, Fang ; Lin, Guanfeng ; Zhang, Deqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c553t-ab25dbfc923d57f3146fa94400340a8125ca4358617d4d4ba7485e42c21012453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aggregation-induced emission</topic><topic>Antibodies, Neutralizing</topic><topic>Antibodies, Viral</topic><topic>COVID-19 - diagnosis</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Lateral flow immunoassay</topic><topic>Nanoparticles</topic><topic>Neutralizing antibody</topic><topic>SARS-CoV-2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bian, Lun</creatorcontrib><creatorcontrib>Li, Zhaoyue</creatorcontrib><creatorcontrib>He, An</creatorcontrib><creatorcontrib>Wu, Biru</creatorcontrib><creatorcontrib>Yang, Hui</creatorcontrib><creatorcontrib>Wu, Yingsong</creatorcontrib><creatorcontrib>Hu, Fang</creatorcontrib><creatorcontrib>Lin, Guanfeng</creatorcontrib><creatorcontrib>Zhang, Deqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bian, Lun</au><au>Li, Zhaoyue</au><au>He, An</au><au>Wu, Biru</au><au>Yang, Hui</au><au>Wu, Yingsong</au><au>Hu, Fang</au><au>Lin, Guanfeng</au><au>Zhang, Deqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrabright nanoparticle-labeled lateral flow immunoassay for detection of anti-SARS-CoV-2 neutralizing antibodies in human serum</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2022-09-01</date><risdate>2022</risdate><volume>288</volume><spage>121694</spage><epage>121694</epage><pages>121694-121694</pages><artnum>121694</artnum><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>The level of anti-SARS-CoV-2 neutralizing antibodies (NAb) is an indispensable reference for evaluating the acquired protective immunity against SARS-CoV-2. Here, we established an ultrabright nanoparticles-based lateral flow immunoassay (LFIA) for one-step rapid semi-quantitative detection of anti-SARS-CoV-2 NAb in vaccinee's serum. Once embedded in polystyrene (PS) nanoparticles, the aggregation-induced emission (AIE) luminogen, AIE490, exhibited ultrabright fluorescence due to the rigidity of PS and severe inhibition of intramolecular motions. The ultrabright AIE490-PS nanoparticle was used as a fluorescent marker of LFIA. Upon optimized conditions including incubation time, concentrations of coated proteins and conjugated nanoparticles, amounts of antigens modified on the surface of nanoparticles, dilution rate of serum samples, and so on, the ultrabright nanoparticles-based LFIA could accurately identify 70 negative samples and 63 positive samples from human serum (p < 0.0001). The intra- and inter-assay precisions of the established method are above 13% and 16%, respectively. 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| subjects | Aggregation-induced emission Antibodies, Neutralizing Antibodies, Viral COVID-19 - diagnosis Humans Immunoassay - methods Lateral flow immunoassay Nanoparticles Neutralizing antibody SARS-CoV-2 |
| title | Ultrabright nanoparticle-labeled lateral flow immunoassay for detection of anti-SARS-CoV-2 neutralizing antibodies in human serum |
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