Paediatric pre‐B acute lymphoblastic leukaemia‐derived exosomes regulate immune function in human T cells
Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia‐derived exosomes has less been investigated. Hence, changes in immune response‐related genes and human T cells apoptosis co‐incubated with exosomes isolated from p...
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| Veröffentlicht in: | Journal of cellular and molecular medicine 2022-08, Vol.26 (16), p.4566-4576 |
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| creator | Gholipour, Elham Kahroba, Houman Soltani, Nasim Samadi, Parisa Sarvarian, Parisa Vakili‐Samiani, Sajjad Hosein Pour Feizi, Abbas Ali Soltani‐Zangbar, Mohammad Sadegh Baghersalimi, Adel Darbandi, Bahram Movassaghpour, Aliakbar Talebi, Mehdi Motavalli, Roza Mehdizadeh, Amir Kazemi, Abdolhassan Yousefi, Mehdi |
| description | Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia‐derived exosomes has less been investigated. Hence, changes in immune response‐related genes and human T cells apoptosis co‐incubated with exosomes isolated from patients' pre‐B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co‐incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT‐PCR. Apoptosis and caspase‐3 and caspase‐9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs‐related cytokines, including TGF‐B and IL‐10. The expression level of Th17‐related transcription factors (RoRγt) and interleukins (IL‐17 and IL‐23) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging. |
| doi_str_mv | 10.1111/jcmm.17482 |
| format | Article |
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Hence, changes in immune response‐related genes and human T cells apoptosis co‐incubated with exosomes isolated from patients' pre‐B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co‐incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT‐PCR. Apoptosis and caspase‐3 and caspase‐9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs‐related cytokines, including TGF‐B and IL‐10. The expression level of Th17‐related transcription factors (RoRγt) and interleukins (IL‐17 and IL‐23) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/jcmm.17482</identifier><identifier>PMID: 35822529</identifier><language>eng</language><publisher>Chichester: John Wiley & Sons, Inc</publisher><subject>Acute lymphoblastic leukemia ; Angiogenesis ; Antibodies ; Apoptosis ; Blood ; Cancer therapies ; Caspase ; CD8+ T cells ; Chemotherapy ; Exosomes ; Foxp3 protein ; Helper cells ; Hematology ; Immune system ; immunosuppression ; Interleukins ; Leukemia ; Lymphocytes ; Lymphocytes T ; Membranes ; Metastases ; mRNA ; Original ; Patients ; Proteins ; regulatory T cell ; Solid tumors ; Transcription factors ; Transmission electron microscopy ; tumour‐derived exosome ; Ultrafiltration ; Veins & arteries ; Western blotting</subject><ispartof>Journal of cellular and molecular medicine, 2022-08, Vol.26 (16), p.4566-4576</ispartof><rights>2022 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4252-51a7fcd7d5c0cbcf7620c83bdc92f8b92da38364d1e4ac9b1b9e7098ee7f589e3</citedby><cites>FETCH-LOGICAL-c4252-51a7fcd7d5c0cbcf7620c83bdc92f8b92da38364d1e4ac9b1b9e7098ee7f589e3</cites><orcidid>0000-0002-3029-4172 ; 0000-0003-3960-5712 ; 0000-0003-0099-6728</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357647/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357647/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11542,27903,27904,45553,45554,46030,46454,53769,53771</link.rule.ids></links><search><creatorcontrib>Gholipour, Elham</creatorcontrib><creatorcontrib>Kahroba, Houman</creatorcontrib><creatorcontrib>Soltani, Nasim</creatorcontrib><creatorcontrib>Samadi, Parisa</creatorcontrib><creatorcontrib>Sarvarian, Parisa</creatorcontrib><creatorcontrib>Vakili‐Samiani, Sajjad</creatorcontrib><creatorcontrib>Hosein Pour Feizi, Abbas Ali</creatorcontrib><creatorcontrib>Soltani‐Zangbar, Mohammad Sadegh</creatorcontrib><creatorcontrib>Baghersalimi, Adel</creatorcontrib><creatorcontrib>Darbandi, Bahram</creatorcontrib><creatorcontrib>Movassaghpour, Aliakbar</creatorcontrib><creatorcontrib>Talebi, Mehdi</creatorcontrib><creatorcontrib>Motavalli, Roza</creatorcontrib><creatorcontrib>Mehdizadeh, Amir</creatorcontrib><creatorcontrib>Kazemi, Abdolhassan</creatorcontrib><creatorcontrib>Yousefi, Mehdi</creatorcontrib><title>Paediatric pre‐B acute lymphoblastic leukaemia‐derived exosomes regulate immune function in human T cells</title><title>Journal of cellular and molecular medicine</title><description>Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia‐derived exosomes has less been investigated. Hence, changes in immune response‐related genes and human T cells apoptosis co‐incubated with exosomes isolated from patients' pre‐B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co‐incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT‐PCR. Apoptosis and caspase‐3 and caspase‐9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs‐related cytokines, including TGF‐B and IL‐10. The expression level of Th17‐related transcription factors (RoRγt) and interleukins (IL‐17 and IL‐23) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging.</description><subject>Acute lymphoblastic leukemia</subject><subject>Angiogenesis</subject><subject>Antibodies</subject><subject>Apoptosis</subject><subject>Blood</subject><subject>Cancer therapies</subject><subject>Caspase</subject><subject>CD8+ T cells</subject><subject>Chemotherapy</subject><subject>Exosomes</subject><subject>Foxp3 protein</subject><subject>Helper cells</subject><subject>Hematology</subject><subject>Immune system</subject><subject>immunosuppression</subject><subject>Interleukins</subject><subject>Leukemia</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Membranes</subject><subject>Metastases</subject><subject>mRNA</subject><subject>Original</subject><subject>Patients</subject><subject>Proteins</subject><subject>regulatory T cell</subject><subject>Solid tumors</subject><subject>Transcription factors</subject><subject>Transmission electron microscopy</subject><subject>tumour‐derived exosome</subject><subject>Ultrafiltration</subject><subject>Veins & arteries</subject><subject>Western 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pre‐B acute lymphoblastic leukaemia‐derived exosomes regulate immune function in human T cells</title><author>Gholipour, Elham ; Kahroba, Houman ; Soltani, Nasim ; Samadi, Parisa ; Sarvarian, Parisa ; Vakili‐Samiani, Sajjad ; Hosein Pour Feizi, Abbas Ali ; Soltani‐Zangbar, Mohammad Sadegh ; Baghersalimi, Adel ; Darbandi, Bahram ; Movassaghpour, Aliakbar ; Talebi, Mehdi ; Motavalli, Roza ; Mehdizadeh, Amir ; Kazemi, Abdolhassan ; Yousefi, Mehdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4252-51a7fcd7d5c0cbcf7620c83bdc92f8b92da38364d1e4ac9b1b9e7098ee7f589e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acute lymphoblastic leukemia</topic><topic>Angiogenesis</topic><topic>Antibodies</topic><topic>Apoptosis</topic><topic>Blood</topic><topic>Cancer therapies</topic><topic>Caspase</topic><topic>CD8+ T 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Hence, changes in immune response‐related genes and human T cells apoptosis co‐incubated with exosomes isolated from patients' pre‐B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co‐incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT‐PCR. Apoptosis and caspase‐3 and caspase‐9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs‐related cytokines, including TGF‐B and IL‐10. 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| source | DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acute lymphoblastic leukemia Angiogenesis Antibodies Apoptosis Blood Cancer therapies Caspase CD8+ T cells Chemotherapy Exosomes Foxp3 protein Helper cells Hematology Immune system immunosuppression Interleukins Leukemia Lymphocytes Lymphocytes T Membranes Metastases mRNA Original Patients Proteins regulatory T cell Solid tumors Transcription factors Transmission electron microscopy tumour‐derived exosome Ultrafiltration Veins & arteries Western blotting |
| title | Paediatric pre‐B acute lymphoblastic leukaemia‐derived exosomes regulate immune function in human T cells |
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