Towards Control and Oversight of SARS‐CoV‐2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors
The development of versatile and sensitive biotools to quantify specific SARS‐CoV‐2 immunoglobulins in SARS‐CoV‐2 infected and non‐infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in‐house expressed recombinant spike (S) proteins is reported....
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| creator | Torrente‐Rodríguez, Rebeca M. Montero‐Calle, Ana San Bartolomé, Clara Cano, Olga Vázquez, Mónica Iglesias‐Caballero, María Corral‐Lugo, Andrés McConnell, Michael J. Pascal, Mariona Mas, Vicente Pingarrón, José M. Barderas, Rodrigo Campuzano, Susana |
| description | The development of versatile and sensitive biotools to quantify specific SARS‐CoV‐2 immunoglobulins in SARS‐CoV‐2 infected and non‐infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in‐house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N‐ and S‐specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP‐conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen‐printed electrodes using the HQ/HRP/H2O2 system. The obtained results using N and in‐house expressed S ectodomains of five SARS‐CoV‐2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.
A multiplexed amperometric bioplatform can detect and quantify total and isotype N‐ and S‐specific anti‐SARS‐CoV‐2 serum immunoglobulins within 75 minutes. The platform was used to analyze the global and isotype‐specific immune response of COVID‐19 convalescent and vaccinated individuals, and to detect variant‐specific S antibodies from variants of concern. |
| doi_str_mv | 10.1002/anie.202203662 |
| format | Article |
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A multiplexed amperometric bioplatform can detect and quantify total and isotype N‐ and S‐specific anti‐SARS‐CoV‐2 serum immunoglobulins within 75 minutes. The platform was used to analyze the global and isotype‐specific immune response of COVID‐19 convalescent and vaccinated individuals, and to detect variant‐specific S antibodies from variants of concern.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.202203662</identifier><identifier>PMID: 35507573</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Antibodies ; Biosensors ; Electrical measurement ; Electroanalytical Bioplatforms ; Hydrogen peroxide ; Immune Response ; Immune system ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Immunoglobulins ; Infections ; Interrogation ; Isotypes ; Microspheres ; Monitoring ; Multiplexing ; Nanoparticles ; Nucleocapsids ; Omicron ; SARS-Cov-2 ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Vaccines ; Viral Antigens</subject><ispartof>Angewandte Chemie International Edition, 2022-07, Vol.61 (28), p.e202203662-n/a</ispartof><rights>2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH</rights><rights>2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4682-3fa3b2d623ddc8fa1dd8c361757c6ed94a4afdd6ce829a01a065544ef809dc473</citedby><cites>FETCH-LOGICAL-c4682-3fa3b2d623ddc8fa1dd8c361757c6ed94a4afdd6ce829a01a065544ef809dc473</cites><orcidid>0000-0003-2271-1383 ; 0000-0002-2153-172X ; 0000-0003-3539-7469 ; 0000-0002-9928-6613 ; 0000-0001-5141-0454</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.202203662$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.202203662$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35507573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torrente‐Rodríguez, Rebeca M.</creatorcontrib><creatorcontrib>Montero‐Calle, Ana</creatorcontrib><creatorcontrib>San Bartolomé, Clara</creatorcontrib><creatorcontrib>Cano, Olga</creatorcontrib><creatorcontrib>Vázquez, Mónica</creatorcontrib><creatorcontrib>Iglesias‐Caballero, María</creatorcontrib><creatorcontrib>Corral‐Lugo, Andrés</creatorcontrib><creatorcontrib>McConnell, Michael J.</creatorcontrib><creatorcontrib>Pascal, Mariona</creatorcontrib><creatorcontrib>Mas, Vicente</creatorcontrib><creatorcontrib>Pingarrón, José M.</creatorcontrib><creatorcontrib>Barderas, Rodrigo</creatorcontrib><creatorcontrib>Campuzano, Susana</creatorcontrib><title>Towards Control and Oversight of SARS‐CoV‐2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors</title><title>Angewandte Chemie International Edition</title><addtitle>Angew Chem Int Ed Engl</addtitle><description>The development of versatile and sensitive biotools to quantify specific SARS‐CoV‐2 immunoglobulins in SARS‐CoV‐2 infected and non‐infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in‐house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N‐ and S‐specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP‐conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen‐printed electrodes using the HQ/HRP/H2O2 system. The obtained results using N and in‐house expressed S ectodomains of five SARS‐CoV‐2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.
A multiplexed amperometric bioplatform can detect and quantify total and isotype N‐ and S‐specific anti‐SARS‐CoV‐2 serum immunoglobulins within 75 minutes. 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Amperometric interrogation of captured N‐ and S‐specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP‐conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen‐printed electrodes using the HQ/HRP/H2O2 system. The obtained results using N and in‐house expressed S ectodomains of five SARS‐CoV‐2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.
A multiplexed amperometric bioplatform can detect and quantify total and isotype N‐ and S‐specific anti‐SARS‐CoV‐2 serum immunoglobulins within 75 minutes. The platform was used to analyze the global and isotype‐specific immune response of COVID‐19 convalescent and vaccinated individuals, and to detect variant‐specific S antibodies from variants of concern.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>35507573</pmid><doi>10.1002/anie.202203662</doi><tpages>9</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0003-2271-1383</orcidid><orcidid>https://orcid.org/0000-0002-2153-172X</orcidid><orcidid>https://orcid.org/0000-0003-3539-7469</orcidid><orcidid>https://orcid.org/0000-0002-9928-6613</orcidid><orcidid>https://orcid.org/0000-0001-5141-0454</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Biosensors Electrical measurement Electroanalytical Bioplatforms Hydrogen peroxide Immune Response Immune system Immunoglobulin A Immunoglobulin G Immunoglobulin M Immunoglobulins Infections Interrogation Isotypes Microspheres Monitoring Multiplexing Nanoparticles Nucleocapsids Omicron SARS-Cov-2 Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Vaccines Viral Antigens |
| title | Towards Control and Oversight of SARS‐CoV‐2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors |
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