Induction of amphotericin B resistance in susceptible Candida auris by extracellular vesicles
Drug resistance derived from extracellular vesicles (EVs) is an increasingly important research area but has seldom been described regarding fungal pathogens. Here, we characterized EVs derived from a triazole-resistant but amphotericin B-susceptible strain of Candida auris. Nano- to microgram conce...
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| Veröffentlicht in: | Emerging microbes & infections 2022-12, Vol.11 (1), p.1900-1909 |
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| creator | Chan, Walton Chow, Franklin Wang-Ngai Tsang, Chi-Ching Liu, Xueyan Yao, Weiming Chan, Tony Tat-Yin Siu, Gilman Kit-Hang Ho, Alex Yat-Man Luk, Kristine Shik Lau, Susanna Kar-Pui Woo, Patrick Chiu-Yat |
| description | Drug resistance derived from extracellular vesicles (EVs) is an increasingly important research area but has seldom been described regarding fungal pathogens. Here, we characterized EVs derived from a triazole-resistant but amphotericin B-susceptible strain of Candida auris. Nano- to microgram concentrations of C. auris EVs prepared from both broth and solid agar cultures could robustly increase the yeast's survival against both pure and clinical amphotericin B formulations in a dose-dependent manner, resulting in up to 16-fold changes of minimum inhibitory concentration. Meanwhile, this effect was not observed upon addition of these EVs to C. albicans, nor upon addition of C. albicans EVs to C. auris. No change in susceptibilities was observed upon EV treatment for fluconazole, voriconazole, micafungin, and flucytosine. Mass spectrometry indicated the presence of immunogenic-/drug resistance-implicated proteins in C. auris EVs, including alcohol dehydrogenase 1 as well as C. albicans Mp65-like and Xog1-like proteins in high quantities. Based on these observations, we propose a potential species-specific role for EVs in amphotericin B resistance in C. auris. These observations may provide critical insights into treatment of multidrug-resistant C. auris. |
| doi_str_mv | 10.1080/22221751.2022.2098058 |
| format | Article |
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Here, we characterized EVs derived from a triazole-resistant but amphotericin B-susceptible strain of Candida auris. Nano- to microgram concentrations of C. auris EVs prepared from both broth and solid agar cultures could robustly increase the yeast's survival against both pure and clinical amphotericin B formulations in a dose-dependent manner, resulting in up to 16-fold changes of minimum inhibitory concentration. Meanwhile, this effect was not observed upon addition of these EVs to C. albicans, nor upon addition of C. albicans EVs to C. auris. No change in susceptibilities was observed upon EV treatment for fluconazole, voriconazole, micafungin, and flucytosine. Mass spectrometry indicated the presence of immunogenic-/drug resistance-implicated proteins in C. auris EVs, including alcohol dehydrogenase 1 as well as C. albicans Mp65-like and Xog1-like proteins in high quantities. Based on these observations, we propose a potential species-specific role for EVs in amphotericin B resistance in C. auris. These observations may provide critical insights into treatment of multidrug-resistant C. auris.</description><identifier>ISSN: 2222-1751</identifier><identifier>EISSN: 2222-1751</identifier><identifier>DOI: 10.1080/22221751.2022.2098058</identifier><identifier>PMID: 35786393</identifier><language>eng</language><publisher>London: Taylor & Francis</publisher><subject>amphotericin B ; Antifungal agents ; Candida auris ; Drug dosages ; Drug resistance ; Epidemics ; Extracellular vesicles ; Fungal infections ; Hospitals ; Life sciences ; Medicine ; solid media EV purification</subject><ispartof>Emerging microbes & infections, 2022-12, Vol.11 (1), p.1900-1909</ispartof><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2022</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution – Non-Commercial License http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2022 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-1dd6f9171df6ba39707e73d806e49bc5c21392b3ab6ef9652b2f64c858bca9fc3</citedby><cites>FETCH-LOGICAL-c539t-1dd6f9171df6ba39707e73d806e49bc5c21392b3ab6ef9652b2f64c858bca9fc3</cites><orcidid>0000-0001-9401-1832 ; 0000-0001-6705-2866 ; 0000-0002-6430-1211 ; 0000-0002-4354-3393 ; 0000-0002-1383-7374 ; 0000-0003-0909-8414 ; 0000-0003-3756-9172 ; 0000-0003-1275-2464 ; 0000-0003-3982-1597</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341352/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341352/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,27502,27924,27925,53791,53793,59143,59144</link.rule.ids></links><search><creatorcontrib>Chan, Walton</creatorcontrib><creatorcontrib>Chow, Franklin Wang-Ngai</creatorcontrib><creatorcontrib>Tsang, Chi-Ching</creatorcontrib><creatorcontrib>Liu, Xueyan</creatorcontrib><creatorcontrib>Yao, Weiming</creatorcontrib><creatorcontrib>Chan, Tony Tat-Yin</creatorcontrib><creatorcontrib>Siu, Gilman Kit-Hang</creatorcontrib><creatorcontrib>Ho, Alex Yat-Man</creatorcontrib><creatorcontrib>Luk, Kristine Shik</creatorcontrib><creatorcontrib>Lau, Susanna Kar-Pui</creatorcontrib><creatorcontrib>Woo, Patrick Chiu-Yat</creatorcontrib><title>Induction of amphotericin B resistance in susceptible Candida auris by extracellular vesicles</title><title>Emerging microbes & infections</title><description>Drug resistance derived from extracellular vesicles (EVs) is an increasingly important research area but has seldom been described regarding fungal pathogens. Here, we characterized EVs derived from a triazole-resistant but amphotericin B-susceptible strain of Candida auris. Nano- to microgram concentrations of C. auris EVs prepared from both broth and solid agar cultures could robustly increase the yeast's survival against both pure and clinical amphotericin B formulations in a dose-dependent manner, resulting in up to 16-fold changes of minimum inhibitory concentration. Meanwhile, this effect was not observed upon addition of these EVs to C. albicans, nor upon addition of C. albicans EVs to C. auris. No change in susceptibilities was observed upon EV treatment for fluconazole, voriconazole, micafungin, and flucytosine. Mass spectrometry indicated the presence of immunogenic-/drug resistance-implicated proteins in C. auris EVs, including alcohol dehydrogenase 1 as well as C. albicans Mp65-like and Xog1-like proteins in high quantities. Based on these observations, we propose a potential species-specific role for EVs in amphotericin B resistance in C. auris. These observations may provide critical insights into treatment of multidrug-resistant C. auris.</description><subject>amphotericin B</subject><subject>Antifungal agents</subject><subject>Candida auris</subject><subject>Drug dosages</subject><subject>Drug resistance</subject><subject>Epidemics</subject><subject>Extracellular vesicles</subject><subject>Fungal infections</subject><subject>Hospitals</subject><subject>Life sciences</subject><subject>Medicine</subject><subject>solid media EV purification</subject><issn>2222-1751</issn><issn>2222-1751</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNp9UsluFDEQbSEQiUI-AckSFy4TvLS3CwJGLCNF4gJHZJW3xKOe9mB3J8zf42YGRDhQBy9V7z2Vy6_rnhN8RbDCr2gLIjm5opjStmiFuXrUnS_51VJ4_Nf5rLusdYtbSCx60j_tzhiXSjDNzrtvm9HPbkp5RDki2O1v8xRKcmlE71AJNdUJRhdQu9e5urCfkh0CWsPokwcEc0kV2QMKP6YCLgzDPEBBd43ohlCfdU8iDDVcnvaL7uuH91_Wn1bXnz9u1m-vV44zPa2I9yJqIomPwgLTEssgmVdYhF5bxx0lTFPLwIoQteDU0ih6p7iyDnR07KLbHHV9hq3Zl7SDcjAZkvmVyOXGQJmWlgyOjkgHUnkFfW-lVkRG3VuMtZO-h6b1-qi1n-0ueBfG9rLhgejDyphuzU2-M5r1hHHaBF6eBEr-Poc6mV2qy2hgDHmuhgrFMdNC8gZ98Q90m-cytlEZKnuFmeJCNhQ_olzJtZYQ_zRDsFn8YH77wSx-MCc_NN6bIy-NMZcd3OcyeDPBYcgllvatqRr2f4mfrnq7nw</recordid><startdate>20221231</startdate><enddate>20221231</enddate><creator>Chan, Walton</creator><creator>Chow, Franklin Wang-Ngai</creator><creator>Tsang, Chi-Ching</creator><creator>Liu, Xueyan</creator><creator>Yao, Weiming</creator><creator>Chan, Tony Tat-Yin</creator><creator>Siu, Gilman Kit-Hang</creator><creator>Ho, Alex Yat-Man</creator><creator>Luk, Kristine Shik</creator><creator>Lau, Susanna Kar-Pui</creator><creator>Woo, Patrick Chiu-Yat</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-9401-1832</orcidid><orcidid>https://orcid.org/0000-0001-6705-2866</orcidid><orcidid>https://orcid.org/0000-0002-6430-1211</orcidid><orcidid>https://orcid.org/0000-0002-4354-3393</orcidid><orcidid>https://orcid.org/0000-0002-1383-7374</orcidid><orcidid>https://orcid.org/0000-0003-0909-8414</orcidid><orcidid>https://orcid.org/0000-0003-3756-9172</orcidid><orcidid>https://orcid.org/0000-0003-1275-2464</orcidid><orcidid>https://orcid.org/0000-0003-3982-1597</orcidid></search><sort><creationdate>20221231</creationdate><title>Induction of amphotericin B resistance in susceptible Candida auris by extracellular vesicles</title><author>Chan, Walton ; Chow, Franklin Wang-Ngai ; Tsang, Chi-Ching ; Liu, Xueyan ; Yao, Weiming ; Chan, Tony Tat-Yin ; Siu, Gilman Kit-Hang ; Ho, Alex Yat-Man ; Luk, Kristine Shik ; Lau, Susanna Kar-Pui ; Woo, Patrick Chiu-Yat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-1dd6f9171df6ba39707e73d806e49bc5c21392b3ab6ef9652b2f64c858bca9fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>amphotericin B</topic><topic>Antifungal agents</topic><topic>Candida auris</topic><topic>Drug dosages</topic><topic>Drug resistance</topic><topic>Epidemics</topic><topic>Extracellular vesicles</topic><topic>Fungal infections</topic><topic>Hospitals</topic><topic>Life sciences</topic><topic>Medicine</topic><topic>solid media EV purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chan, Walton</creatorcontrib><creatorcontrib>Chow, Franklin Wang-Ngai</creatorcontrib><creatorcontrib>Tsang, Chi-Ching</creatorcontrib><creatorcontrib>Liu, Xueyan</creatorcontrib><creatorcontrib>Yao, Weiming</creatorcontrib><creatorcontrib>Chan, Tony Tat-Yin</creatorcontrib><creatorcontrib>Siu, Gilman Kit-Hang</creatorcontrib><creatorcontrib>Ho, Alex Yat-Man</creatorcontrib><creatorcontrib>Luk, Kristine Shik</creatorcontrib><creatorcontrib>Lau, Susanna Kar-Pui</creatorcontrib><creatorcontrib>Woo, Patrick Chiu-Yat</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Emerging microbes & infections</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chan, Walton</au><au>Chow, Franklin Wang-Ngai</au><au>Tsang, Chi-Ching</au><au>Liu, Xueyan</au><au>Yao, Weiming</au><au>Chan, Tony Tat-Yin</au><au>Siu, Gilman Kit-Hang</au><au>Ho, Alex Yat-Man</au><au>Luk, Kristine Shik</au><au>Lau, Susanna Kar-Pui</au><au>Woo, Patrick Chiu-Yat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of amphotericin B resistance in susceptible Candida auris by extracellular vesicles</atitle><jtitle>Emerging microbes & infections</jtitle><date>2022-12-31</date><risdate>2022</risdate><volume>11</volume><issue>1</issue><spage>1900</spage><epage>1909</epage><pages>1900-1909</pages><issn>2222-1751</issn><eissn>2222-1751</eissn><abstract>Drug resistance derived from extracellular vesicles (EVs) is an increasingly important research area but has seldom been described regarding fungal pathogens. Here, we characterized EVs derived from a triazole-resistant but amphotericin B-susceptible strain of Candida auris. Nano- to microgram concentrations of C. auris EVs prepared from both broth and solid agar cultures could robustly increase the yeast's survival against both pure and clinical amphotericin B formulations in a dose-dependent manner, resulting in up to 16-fold changes of minimum inhibitory concentration. Meanwhile, this effect was not observed upon addition of these EVs to C. albicans, nor upon addition of C. albicans EVs to C. auris. No change in susceptibilities was observed upon EV treatment for fluconazole, voriconazole, micafungin, and flucytosine. Mass spectrometry indicated the presence of immunogenic-/drug resistance-implicated proteins in C. auris EVs, including alcohol dehydrogenase 1 as well as C. albicans Mp65-like and Xog1-like proteins in high quantities. Based on these observations, we propose a potential species-specific role for EVs in amphotericin B resistance in C. auris. These observations may provide critical insights into treatment of multidrug-resistant C. auris.</abstract><cop>London</cop><pub>Taylor & Francis</pub><pmid>35786393</pmid><doi>10.1080/22221751.2022.2098058</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9401-1832</orcidid><orcidid>https://orcid.org/0000-0001-6705-2866</orcidid><orcidid>https://orcid.org/0000-0002-6430-1211</orcidid><orcidid>https://orcid.org/0000-0002-4354-3393</orcidid><orcidid>https://orcid.org/0000-0002-1383-7374</orcidid><orcidid>https://orcid.org/0000-0003-0909-8414</orcidid><orcidid>https://orcid.org/0000-0003-3756-9172</orcidid><orcidid>https://orcid.org/0000-0003-1275-2464</orcidid><orcidid>https://orcid.org/0000-0003-3982-1597</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | amphotericin B Antifungal agents Candida auris Drug dosages Drug resistance Epidemics Extracellular vesicles Fungal infections Hospitals Life sciences Medicine solid media EV purification |
| title | Induction of amphotericin B resistance in susceptible Candida auris by extracellular vesicles |
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