Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System
The cancer cell fusion theory could be one of the best explanations for the metastasis from primary tumours. Herein, we co-cultured colorectal cancer (CRC) stem cells with human monocytes and analysed the properties of the generated tumour hybrid cells (THCs). The presence of THCs in the bloodstream...
Gespeichert in:
| Veröffentlicht in: | Cancers 2022-07, Vol.14 (14), p.3445 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 14 |
| container_start_page | 3445 |
| container_title | Cancers |
| container_volume | 14 |
| creator | Montalbán-Hernández, Karla Cantero-Cid, Ramón Casalvilla-Dueñas, José Carlos Avendaño-Ortiz, José Marín, Elvira Lozano-Rodríguez, Roberto Terrón-Arcos, Verónica Vicario-Bravo, Marina Marcano, Cristóbal Saavedra-Ambrosy, Jorge Prado-Montero, Julia Valentín, Jaime Pérez de Diego, Rebeca Córdoba, Laura Pulido, Elisa Del Fresno, Carlos Dueñas, Marta López-Collazo, Eduardo |
| description | The cancer cell fusion theory could be one of the best explanations for the metastasis from primary tumours.
Herein, we co-cultured colorectal cancer (CRC) stem cells with human monocytes and analysed the properties of the generated tumour hybrid cells (THCs). The presence of THCs in the bloodstream together with samples from primary and metastatic lesions and their clinical correlations were evaluated in CRC patients and were detected by both FACS and immunofluorescence methods. Additionally, the role of SIGLEC5 as an immune evasion molecule in colorectal cancer was evaluated.
Our data demonstrated the generation of THCs after the in vitro co-culture of CRC stem cells and monocytes. These cells, defined as CD45
CD14
EpCAM
, showed enhanced migratory and proliferative abilities. The THC-specific cell surface signature allows identification in matched primary tumour tissues and metastases as well as in the bloodstream from patients with CRC, thus functioning as a biomarker. Moreover, SIG-LEC5 expression on in vitro generated THCs has shown to be involved in the mechanism for immune evasion. Additionally, sSIGLEC5 levels correlated with THC numbers in the prospective cohort of patients.
Our results indicate the generation of a hybrid entity after the in vitro co-culture between CRC stem cells and human monocytes. Moreover, THC numbers present in patients are related to both prognosis and the later spread of metastases in CRC patients. |
| doi_str_mv | 10.3390/cancers14143445 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9324286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2693942266</sourcerecordid><originalsourceid>FETCH-LOGICAL-c351t-9f6b2196b6b324823a395a604a743603d50e7781d4ccffca76a20152e0029023</originalsourceid><addsrcrecordid>eNpdkc1P3DAQxa2qVUGUc2-VpV562eLvxJdKKGILEqgH9m45zoQ1SmJqO6Dc-4fXu2wRxRdbmp_fzJuH0GdKvnOuyZmzk4OYqKCCCyHfoWNGKrZSSov3r95H6DSle1IO57RS1Ud0xGVdC0nkMfrThCFEcNkOuNnr4dsMI25gGBJezwnwk89bfBOm4JYMCeeA1yGOeDOPYY74cmmj7w78Hs1bwOetH3xedvCNv4s2A7ZThy8ebQd74Goc5wnw7ZJKt0_oQ2-HBKeH-wRt1heb5nJ1_evnVXN-vXJc0rzSvWoZ1apVLWeiZtxyLa0iwlaCK8I7SaCqatoJ5_re2UpZRqhkQAjThPET9ONZ9mFuR-gcTDnawTxEP9q4mGC9-b8y-a25C49Gl3asVkXg20Eght8zpGxGn1xxbicIczJMacl0xeq6oF_foPdlW1Nxt6O4FoypneDZM-ViSClC_zIMJWaXsXmTcfnx5bWHF_5fovwvMpOjpw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2693942266</pqid></control><display><type>article</type><title>Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System</title><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Montalbán-Hernández, Karla ; Cantero-Cid, Ramón ; Casalvilla-Dueñas, José Carlos ; Avendaño-Ortiz, José ; Marín, Elvira ; Lozano-Rodríguez, Roberto ; Terrón-Arcos, Verónica ; Vicario-Bravo, Marina ; Marcano, Cristóbal ; Saavedra-Ambrosy, Jorge ; Prado-Montero, Julia ; Valentín, Jaime ; Pérez de Diego, Rebeca ; Córdoba, Laura ; Pulido, Elisa ; Del Fresno, Carlos ; Dueñas, Marta ; López-Collazo, Eduardo</creator><creatorcontrib>Montalbán-Hernández, Karla ; Cantero-Cid, Ramón ; Casalvilla-Dueñas, José Carlos ; Avendaño-Ortiz, José ; Marín, Elvira ; Lozano-Rodríguez, Roberto ; Terrón-Arcos, Verónica ; Vicario-Bravo, Marina ; Marcano, Cristóbal ; Saavedra-Ambrosy, Jorge ; Prado-Montero, Julia ; Valentín, Jaime ; Pérez de Diego, Rebeca ; Córdoba, Laura ; Pulido, Elisa ; Del Fresno, Carlos ; Dueñas, Marta ; López-Collazo, Eduardo</creatorcontrib><description>The cancer cell fusion theory could be one of the best explanations for the metastasis from primary tumours.
Herein, we co-cultured colorectal cancer (CRC) stem cells with human monocytes and analysed the properties of the generated tumour hybrid cells (THCs). The presence of THCs in the bloodstream together with samples from primary and metastatic lesions and their clinical correlations were evaluated in CRC patients and were detected by both FACS and immunofluorescence methods. Additionally, the role of SIGLEC5 as an immune evasion molecule in colorectal cancer was evaluated.
Our data demonstrated the generation of THCs after the in vitro co-culture of CRC stem cells and monocytes. These cells, defined as CD45
CD14
EpCAM
, showed enhanced migratory and proliferative abilities. The THC-specific cell surface signature allows identification in matched primary tumour tissues and metastases as well as in the bloodstream from patients with CRC, thus functioning as a biomarker. Moreover, SIG-LEC5 expression on in vitro generated THCs has shown to be involved in the mechanism for immune evasion. Additionally, sSIGLEC5 levels correlated with THC numbers in the prospective cohort of patients.
Our results indicate the generation of a hybrid entity after the in vitro co-culture between CRC stem cells and human monocytes. Moreover, THC numbers present in patients are related to both prognosis and the later spread of metastases in CRC patients.</description><identifier>ISSN: 2072-6694</identifier><identifier>EISSN: 2072-6694</identifier><identifier>DOI: 10.3390/cancers14143445</identifier><identifier>PMID: 35884505</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Biomarkers ; Blood & organ donations ; Cancer ; CD14 antigen ; CD45 antigen ; Cell culture ; Cell fusion ; Cell surface ; Colorectal cancer ; Colorectal carcinoma ; Flow cytometry ; Immune system ; Immunofluorescence ; Medical prognosis ; Metastases ; Metastasis ; Monocytes ; Patients ; Prognosis ; Stem cells ; Surgery ; Transcription factors ; Tumors</subject><ispartof>Cancers, 2022-07, Vol.14 (14), p.3445</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-9f6b2196b6b324823a395a604a743603d50e7781d4ccffca76a20152e0029023</citedby><cites>FETCH-LOGICAL-c351t-9f6b2196b6b324823a395a604a743603d50e7781d4ccffca76a20152e0029023</cites><orcidid>0000-0003-1771-7254 ; 0000-0002-1612-103X ; 0000-0003-2206-2328 ; 0000-0003-2774-1821 ; 0000-0003-0093-9707 ; 0000-0003-0857-9726</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324286/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324286/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35884505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Montalbán-Hernández, Karla</creatorcontrib><creatorcontrib>Cantero-Cid, Ramón</creatorcontrib><creatorcontrib>Casalvilla-Dueñas, José Carlos</creatorcontrib><creatorcontrib>Avendaño-Ortiz, José</creatorcontrib><creatorcontrib>Marín, Elvira</creatorcontrib><creatorcontrib>Lozano-Rodríguez, Roberto</creatorcontrib><creatorcontrib>Terrón-Arcos, Verónica</creatorcontrib><creatorcontrib>Vicario-Bravo, Marina</creatorcontrib><creatorcontrib>Marcano, Cristóbal</creatorcontrib><creatorcontrib>Saavedra-Ambrosy, Jorge</creatorcontrib><creatorcontrib>Prado-Montero, Julia</creatorcontrib><creatorcontrib>Valentín, Jaime</creatorcontrib><creatorcontrib>Pérez de Diego, Rebeca</creatorcontrib><creatorcontrib>Córdoba, Laura</creatorcontrib><creatorcontrib>Pulido, Elisa</creatorcontrib><creatorcontrib>Del Fresno, Carlos</creatorcontrib><creatorcontrib>Dueñas, Marta</creatorcontrib><creatorcontrib>López-Collazo, Eduardo</creatorcontrib><title>Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System</title><title>Cancers</title><addtitle>Cancers (Basel)</addtitle><description>The cancer cell fusion theory could be one of the best explanations for the metastasis from primary tumours.
Herein, we co-cultured colorectal cancer (CRC) stem cells with human monocytes and analysed the properties of the generated tumour hybrid cells (THCs). The presence of THCs in the bloodstream together with samples from primary and metastatic lesions and their clinical correlations were evaluated in CRC patients and were detected by both FACS and immunofluorescence methods. Additionally, the role of SIGLEC5 as an immune evasion molecule in colorectal cancer was evaluated.
Our data demonstrated the generation of THCs after the in vitro co-culture of CRC stem cells and monocytes. These cells, defined as CD45
CD14
EpCAM
, showed enhanced migratory and proliferative abilities. The THC-specific cell surface signature allows identification in matched primary tumour tissues and metastases as well as in the bloodstream from patients with CRC, thus functioning as a biomarker. Moreover, SIG-LEC5 expression on in vitro generated THCs has shown to be involved in the mechanism for immune evasion. Additionally, sSIGLEC5 levels correlated with THC numbers in the prospective cohort of patients.
Our results indicate the generation of a hybrid entity after the in vitro co-culture between CRC stem cells and human monocytes. Moreover, THC numbers present in patients are related to both prognosis and the later spread of metastases in CRC patients.</description><subject>Biomarkers</subject><subject>Blood & organ donations</subject><subject>Cancer</subject><subject>CD14 antigen</subject><subject>CD45 antigen</subject><subject>Cell culture</subject><subject>Cell fusion</subject><subject>Cell surface</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Flow cytometry</subject><subject>Immune system</subject><subject>Immunofluorescence</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Monocytes</subject><subject>Patients</subject><subject>Prognosis</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Transcription factors</subject><subject>Tumors</subject><issn>2072-6694</issn><issn>2072-6694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1P3DAQxa2qVUGUc2-VpV562eLvxJdKKGILEqgH9m45zoQ1SmJqO6Dc-4fXu2wRxRdbmp_fzJuH0GdKvnOuyZmzk4OYqKCCCyHfoWNGKrZSSov3r95H6DSle1IO57RS1Ud0xGVdC0nkMfrThCFEcNkOuNnr4dsMI25gGBJezwnwk89bfBOm4JYMCeeA1yGOeDOPYY74cmmj7w78Hs1bwOetH3xedvCNv4s2A7ZThy8ebQd74Goc5wnw7ZJKt0_oQ2-HBKeH-wRt1heb5nJ1_evnVXN-vXJc0rzSvWoZ1apVLWeiZtxyLa0iwlaCK8I7SaCqatoJ5_re2UpZRqhkQAjThPET9ONZ9mFuR-gcTDnawTxEP9q4mGC9-b8y-a25C49Gl3asVkXg20Eght8zpGxGn1xxbicIczJMacl0xeq6oF_foPdlW1Nxt6O4FoypneDZM-ViSClC_zIMJWaXsXmTcfnx5bWHF_5fovwvMpOjpw</recordid><startdate>20220715</startdate><enddate>20220715</enddate><creator>Montalbán-Hernández, Karla</creator><creator>Cantero-Cid, Ramón</creator><creator>Casalvilla-Dueñas, José Carlos</creator><creator>Avendaño-Ortiz, José</creator><creator>Marín, Elvira</creator><creator>Lozano-Rodríguez, Roberto</creator><creator>Terrón-Arcos, Verónica</creator><creator>Vicario-Bravo, Marina</creator><creator>Marcano, Cristóbal</creator><creator>Saavedra-Ambrosy, Jorge</creator><creator>Prado-Montero, Julia</creator><creator>Valentín, Jaime</creator><creator>Pérez de Diego, Rebeca</creator><creator>Córdoba, Laura</creator><creator>Pulido, Elisa</creator><creator>Del Fresno, Carlos</creator><creator>Dueñas, Marta</creator><creator>López-Collazo, Eduardo</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1771-7254</orcidid><orcidid>https://orcid.org/0000-0002-1612-103X</orcidid><orcidid>https://orcid.org/0000-0003-2206-2328</orcidid><orcidid>https://orcid.org/0000-0003-2774-1821</orcidid><orcidid>https://orcid.org/0000-0003-0093-9707</orcidid><orcidid>https://orcid.org/0000-0003-0857-9726</orcidid></search><sort><creationdate>20220715</creationdate><title>Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System</title><author>Montalbán-Hernández, Karla ; Cantero-Cid, Ramón ; Casalvilla-Dueñas, José Carlos ; Avendaño-Ortiz, José ; Marín, Elvira ; Lozano-Rodríguez, Roberto ; Terrón-Arcos, Verónica ; Vicario-Bravo, Marina ; Marcano, Cristóbal ; Saavedra-Ambrosy, Jorge ; Prado-Montero, Julia ; Valentín, Jaime ; Pérez de Diego, Rebeca ; Córdoba, Laura ; Pulido, Elisa ; Del Fresno, Carlos ; Dueñas, Marta ; López-Collazo, Eduardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-9f6b2196b6b324823a395a604a743603d50e7781d4ccffca76a20152e0029023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomarkers</topic><topic>Blood & organ donations</topic><topic>Cancer</topic><topic>CD14 antigen</topic><topic>CD45 antigen</topic><topic>Cell culture</topic><topic>Cell fusion</topic><topic>Cell surface</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Flow cytometry</topic><topic>Immune system</topic><topic>Immunofluorescence</topic><topic>Medical prognosis</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Monocytes</topic><topic>Patients</topic><topic>Prognosis</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Transcription factors</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Montalbán-Hernández, Karla</creatorcontrib><creatorcontrib>Cantero-Cid, Ramón</creatorcontrib><creatorcontrib>Casalvilla-Dueñas, José Carlos</creatorcontrib><creatorcontrib>Avendaño-Ortiz, José</creatorcontrib><creatorcontrib>Marín, Elvira</creatorcontrib><creatorcontrib>Lozano-Rodríguez, Roberto</creatorcontrib><creatorcontrib>Terrón-Arcos, Verónica</creatorcontrib><creatorcontrib>Vicario-Bravo, Marina</creatorcontrib><creatorcontrib>Marcano, Cristóbal</creatorcontrib><creatorcontrib>Saavedra-Ambrosy, Jorge</creatorcontrib><creatorcontrib>Prado-Montero, Julia</creatorcontrib><creatorcontrib>Valentín, Jaime</creatorcontrib><creatorcontrib>Pérez de Diego, Rebeca</creatorcontrib><creatorcontrib>Córdoba, Laura</creatorcontrib><creatorcontrib>Pulido, Elisa</creatorcontrib><creatorcontrib>Del Fresno, Carlos</creatorcontrib><creatorcontrib>Dueñas, Marta</creatorcontrib><creatorcontrib>López-Collazo, Eduardo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Montalbán-Hernández, Karla</au><au>Cantero-Cid, Ramón</au><au>Casalvilla-Dueñas, José Carlos</au><au>Avendaño-Ortiz, José</au><au>Marín, Elvira</au><au>Lozano-Rodríguez, Roberto</au><au>Terrón-Arcos, Verónica</au><au>Vicario-Bravo, Marina</au><au>Marcano, Cristóbal</au><au>Saavedra-Ambrosy, Jorge</au><au>Prado-Montero, Julia</au><au>Valentín, Jaime</au><au>Pérez de Diego, Rebeca</au><au>Córdoba, Laura</au><au>Pulido, Elisa</au><au>Del Fresno, Carlos</au><au>Dueñas, Marta</au><au>López-Collazo, Eduardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System</atitle><jtitle>Cancers</jtitle><addtitle>Cancers (Basel)</addtitle><date>2022-07-15</date><risdate>2022</risdate><volume>14</volume><issue>14</issue><spage>3445</spage><pages>3445-</pages><issn>2072-6694</issn><eissn>2072-6694</eissn><abstract>The cancer cell fusion theory could be one of the best explanations for the metastasis from primary tumours.
Herein, we co-cultured colorectal cancer (CRC) stem cells with human monocytes and analysed the properties of the generated tumour hybrid cells (THCs). The presence of THCs in the bloodstream together with samples from primary and metastatic lesions and their clinical correlations were evaluated in CRC patients and were detected by both FACS and immunofluorescence methods. Additionally, the role of SIGLEC5 as an immune evasion molecule in colorectal cancer was evaluated.
Our data demonstrated the generation of THCs after the in vitro co-culture of CRC stem cells and monocytes. These cells, defined as CD45
CD14
EpCAM
, showed enhanced migratory and proliferative abilities. The THC-specific cell surface signature allows identification in matched primary tumour tissues and metastases as well as in the bloodstream from patients with CRC, thus functioning as a biomarker. Moreover, SIG-LEC5 expression on in vitro generated THCs has shown to be involved in the mechanism for immune evasion. Additionally, sSIGLEC5 levels correlated with THC numbers in the prospective cohort of patients.
Our results indicate the generation of a hybrid entity after the in vitro co-culture between CRC stem cells and human monocytes. Moreover, THC numbers present in patients are related to both prognosis and the later spread of metastases in CRC patients.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35884505</pmid><doi>10.3390/cancers14143445</doi><orcidid>https://orcid.org/0000-0003-1771-7254</orcidid><orcidid>https://orcid.org/0000-0002-1612-103X</orcidid><orcidid>https://orcid.org/0000-0003-2206-2328</orcidid><orcidid>https://orcid.org/0000-0003-2774-1821</orcidid><orcidid>https://orcid.org/0000-0003-0093-9707</orcidid><orcidid>https://orcid.org/0000-0003-0857-9726</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2072-6694 |
| ispartof | Cancers, 2022-07, Vol.14 (14), p.3445 |
| issn | 2072-6694 2072-6694 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9324286 |
| source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biomarkers Blood & organ donations Cancer CD14 antigen CD45 antigen Cell culture Cell fusion Cell surface Colorectal cancer Colorectal carcinoma Flow cytometry Immune system Immunofluorescence Medical prognosis Metastases Metastasis Monocytes Patients Prognosis Stem cells Surgery Transcription factors Tumors |
| title | Colorectal Cancer Stem Cells Fuse with Monocytes to Form Tumour Hybrid Cells with the Ability to Migrate and Evade the Immune System |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T20%3A34%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Colorectal%20Cancer%20Stem%20Cells%20Fuse%20with%20Monocytes%20to%20Form%20Tumour%20Hybrid%20Cells%20with%20the%20Ability%20to%20Migrate%20and%20Evade%20the%20Immune%20System&rft.jtitle=Cancers&rft.au=Montalb%C3%A1n-Hern%C3%A1ndez,%20Karla&rft.date=2022-07-15&rft.volume=14&rft.issue=14&rft.spage=3445&rft.pages=3445-&rft.issn=2072-6694&rft.eissn=2072-6694&rft_id=info:doi/10.3390/cancers14143445&rft_dat=%3Cproquest_pubme%3E2693942266%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2693942266&rft_id=info:pmid/35884505&rfr_iscdi=true |