Therapeutic effect of paeoniflorin on chronic constriction injury of the sciatic nerve via the inhibition of Schwann cell apoptosis
Therapeutic drugs of chronic neuralgia have a high risk of addiction, making it crucial to identify novel drugs for chronic neuralgia. This study aimed to explore the therapeutic effect of paeoniflorin on chronic sciatica via inhibiting Schwann cell apoptosis. 28 SD rats were randomly divided into f...
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| Veröffentlicht in: | Phytotherapy research 2022-06, Vol.36 (6), p.2572-2582 |
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| creator | Zhang, Di Chang, Shiquan Li, Xin Shi, Huimei Jing, Bei Chen, Zhenni Lin, Yi Zheng, Yachun Qian, Guoqiang Pan, Yuwei Zhao, Guoping |
| description | Therapeutic drugs of chronic neuralgia have a high risk of addiction, making it crucial to identify novel drugs for chronic neuralgia. This study aimed to explore the therapeutic effect of paeoniflorin on chronic sciatica via inhibiting Schwann cell apoptosis. 28 SD rats were randomly divided into four groups, including the sham operation group, chronic constriction injury (CCI) group, mecobalamin group, and paeoniflorin group. The therapeutic effect and mechanism of paeoniflorin were evaluated via rat and cell experiments. Mechanical, hot, or cold hyperalgesia was induced in the rats after CCI operation, while paeoniflorin relieved chronic neuralgia. Besides, paeoniflorin decreased the levels of IL1, IL6, TNF‐α, CRP, and LPS and increased the level of IL10 in serum. As for the sciatic nerve, the number of inflammatory cells was decreased, and Schwann cells were present after paeoniflorin treatment, and paeoniflorin promoted the recovery of nerve structure. In cell experiments, LPS induced Schwann cell apoptosis via the TLR4/NF‐kB pathway. And paeoniflorin attenuated LPS‐induced Schwann cell apoptosis by decreasing the levels of TLR4, p‐NF‐kB, caspase3, cleaved‐caspase3, and cleaved‐caspase7. Overall, these results suggest that paeoniflorin alleviates chronic sciatica by decreasing inflammatory factor levels and promotes the repair of damaged nerves by reducing Schwann cell apoptosis. |
| doi_str_mv | 10.1002/ptr.7472 |
| format | Article |
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This study aimed to explore the therapeutic effect of paeoniflorin on chronic sciatica via inhibiting Schwann cell apoptosis. 28 SD rats were randomly divided into four groups, including the sham operation group, chronic constriction injury (CCI) group, mecobalamin group, and paeoniflorin group. The therapeutic effect and mechanism of paeoniflorin were evaluated via rat and cell experiments. Mechanical, hot, or cold hyperalgesia was induced in the rats after CCI operation, while paeoniflorin relieved chronic neuralgia. Besides, paeoniflorin decreased the levels of IL1, IL6, TNF‐α, CRP, and LPS and increased the level of IL10 in serum. As for the sciatic nerve, the number of inflammatory cells was decreased, and Schwann cells were present after paeoniflorin treatment, and paeoniflorin promoted the recovery of nerve structure. In cell experiments, LPS induced Schwann cell apoptosis via the TLR4/NF‐kB pathway. And paeoniflorin attenuated LPS‐induced Schwann cell apoptosis by decreasing the levels of TLR4, p‐NF‐kB, caspase3, cleaved‐caspase3, and cleaved‐caspase7. Overall, these results suggest that paeoniflorin alleviates chronic sciatica by decreasing inflammatory factor levels and promotes the repair of damaged nerves by reducing Schwann cell apoptosis.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.7472</identifier><identifier>PMID: 35499270</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Addictions ; Apoptosis ; chronic constriction injury ; chronic neuralgia ; Constrictions ; Drugs ; Hyperalgesia ; Inflammation ; Interleukin 1 ; Interleukin 10 ; Interleukin 6 ; Lipopolysaccharides ; Nerves ; Neuralgia ; paeoniflorin ; Pain perception ; Schwann cells ; Sciatic nerve ; Sciatica ; TLR4 protein ; TLR4/NF‐kB ; Toll-like receptors ; Tumor necrosis factor</subject><ispartof>Phytotherapy research, 2022-06, Vol.36 (6), p.2572-2582</ispartof><rights>2022 The Authors. published by John Wiley & Sons Ltd.</rights><rights>2022 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4382-4d3d994bd021f6df7abbdd6ebb0e1a64733c69aafd8dfaa28ee79406d364d2323</citedby><cites>FETCH-LOGICAL-c4382-4d3d994bd021f6df7abbdd6ebb0e1a64733c69aafd8dfaa28ee79406d364d2323</cites><orcidid>0000-0002-8208-2323 ; 0000-0003-0885-8258 ; 0000-0003-1594-2378 ; 0000-0003-3888-9282 ; 0000-0002-3670-9877 ; 0000-0001-9026-5149 ; 0000-0001-9134-6573 ; 0000-0003-3036-1512 ; 0000-0002-5577-2329 ; 0000-0001-8040-8927</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.7472$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.7472$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35499270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Di</creatorcontrib><creatorcontrib>Chang, Shiquan</creatorcontrib><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Shi, Huimei</creatorcontrib><creatorcontrib>Jing, Bei</creatorcontrib><creatorcontrib>Chen, Zhenni</creatorcontrib><creatorcontrib>Lin, Yi</creatorcontrib><creatorcontrib>Zheng, Yachun</creatorcontrib><creatorcontrib>Qian, Guoqiang</creatorcontrib><creatorcontrib>Pan, Yuwei</creatorcontrib><creatorcontrib>Zhao, Guoping</creatorcontrib><title>Therapeutic effect of paeoniflorin on chronic constriction injury of the sciatic nerve via the inhibition of Schwann cell apoptosis</title><title>Phytotherapy research</title><addtitle>Phytother Res</addtitle><description>Therapeutic drugs of chronic neuralgia have a high risk of addiction, making it crucial to identify novel drugs for chronic neuralgia. This study aimed to explore the therapeutic effect of paeoniflorin on chronic sciatica via inhibiting Schwann cell apoptosis. 28 SD rats were randomly divided into four groups, including the sham operation group, chronic constriction injury (CCI) group, mecobalamin group, and paeoniflorin group. The therapeutic effect and mechanism of paeoniflorin were evaluated via rat and cell experiments. Mechanical, hot, or cold hyperalgesia was induced in the rats after CCI operation, while paeoniflorin relieved chronic neuralgia. Besides, paeoniflorin decreased the levels of IL1, IL6, TNF‐α, CRP, and LPS and increased the level of IL10 in serum. As for the sciatic nerve, the number of inflammatory cells was decreased, and Schwann cells were present after paeoniflorin treatment, and paeoniflorin promoted the recovery of nerve structure. In cell experiments, LPS induced Schwann cell apoptosis via the TLR4/NF‐kB pathway. And paeoniflorin attenuated LPS‐induced Schwann cell apoptosis by decreasing the levels of TLR4, p‐NF‐kB, caspase3, cleaved‐caspase3, and cleaved‐caspase7. Overall, these results suggest that paeoniflorin alleviates chronic sciatica by decreasing inflammatory factor levels and promotes the repair of damaged nerves by reducing Schwann cell apoptosis.</description><subject>Addictions</subject><subject>Apoptosis</subject><subject>chronic constriction injury</subject><subject>chronic neuralgia</subject><subject>Constrictions</subject><subject>Drugs</subject><subject>Hyperalgesia</subject><subject>Inflammation</subject><subject>Interleukin 1</subject><subject>Interleukin 10</subject><subject>Interleukin 6</subject><subject>Lipopolysaccharides</subject><subject>Nerves</subject><subject>Neuralgia</subject><subject>paeoniflorin</subject><subject>Pain perception</subject><subject>Schwann cells</subject><subject>Sciatic nerve</subject><subject>Sciatica</subject><subject>TLR4 protein</subject><subject>TLR4/NF‐kB</subject><subject>Toll-like receptors</subject><subject>Tumor necrosis factor</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNp1kU2LFDEQhoMo7jgK_gJp8OKl13x1Z3IRZFk_YEHREbyFdFKxM_QkbdI9y5z946Zn12UVPBVVeXioyovQc4LPCcb09Tilc8EFfYBWBEtZk0awh2iFZUNqTjbfz9CTnHcYY0kxf4zOWMOlpAKv0K9tD0mPME_eVOAcmKmKrho1xODdEJMPVQyV6VPpTWViyFPyZvJl6MNuTscFn3qosvF6kQRIB6gOXp-mPvS-8ye8cF9Nf61D0cEwVHqM4xSzz0_RI6eHDM9u6xp9e3e5vfhQX316__Hi7VVtONvQmltmpeSdxZS41jqhu87aFroOA9EtF4yZVmrt7MY6rekGQEiOW8tabimjbI3e3HjHuduDNRCmpAc1Jr_X6aii9urvl-B79SMelGQUSyaK4NWtIMWfM-RJ7X1ebtEB4pwVbZtNy5vl99fo5T_oLs4plPMKJUQjWiLuCU2KOSdwd8sQrJZkVUlWLckW9MX95e_AP1EWoL4Brv0Ax_-K1Oftl5PwN1thsYg</recordid><startdate>202206</startdate><enddate>202206</enddate><creator>Zhang, Di</creator><creator>Chang, Shiquan</creator><creator>Li, Xin</creator><creator>Shi, Huimei</creator><creator>Jing, Bei</creator><creator>Chen, Zhenni</creator><creator>Lin, Yi</creator><creator>Zheng, Yachun</creator><creator>Qian, Guoqiang</creator><creator>Pan, Yuwei</creator><creator>Zhao, Guoping</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8208-2323</orcidid><orcidid>https://orcid.org/0000-0003-0885-8258</orcidid><orcidid>https://orcid.org/0000-0003-1594-2378</orcidid><orcidid>https://orcid.org/0000-0003-3888-9282</orcidid><orcidid>https://orcid.org/0000-0002-3670-9877</orcidid><orcidid>https://orcid.org/0000-0001-9026-5149</orcidid><orcidid>https://orcid.org/0000-0001-9134-6573</orcidid><orcidid>https://orcid.org/0000-0003-3036-1512</orcidid><orcidid>https://orcid.org/0000-0002-5577-2329</orcidid><orcidid>https://orcid.org/0000-0001-8040-8927</orcidid></search><sort><creationdate>202206</creationdate><title>Therapeutic effect of paeoniflorin on chronic constriction injury of the sciatic nerve via the inhibition of Schwann cell apoptosis</title><author>Zhang, Di ; 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This study aimed to explore the therapeutic effect of paeoniflorin on chronic sciatica via inhibiting Schwann cell apoptosis. 28 SD rats were randomly divided into four groups, including the sham operation group, chronic constriction injury (CCI) group, mecobalamin group, and paeoniflorin group. The therapeutic effect and mechanism of paeoniflorin were evaluated via rat and cell experiments. Mechanical, hot, or cold hyperalgesia was induced in the rats after CCI operation, while paeoniflorin relieved chronic neuralgia. Besides, paeoniflorin decreased the levels of IL1, IL6, TNF‐α, CRP, and LPS and increased the level of IL10 in serum. As for the sciatic nerve, the number of inflammatory cells was decreased, and Schwann cells were present after paeoniflorin treatment, and paeoniflorin promoted the recovery of nerve structure. In cell experiments, LPS induced Schwann cell apoptosis via the TLR4/NF‐kB pathway. And paeoniflorin attenuated LPS‐induced Schwann cell apoptosis by decreasing the levels of TLR4, p‐NF‐kB, caspase3, cleaved‐caspase3, and cleaved‐caspase7. Overall, these results suggest that paeoniflorin alleviates chronic sciatica by decreasing inflammatory factor levels and promotes the repair of damaged nerves by reducing Schwann cell apoptosis.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>35499270</pmid><doi>10.1002/ptr.7472</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8208-2323</orcidid><orcidid>https://orcid.org/0000-0003-0885-8258</orcidid><orcidid>https://orcid.org/0000-0003-1594-2378</orcidid><orcidid>https://orcid.org/0000-0003-3888-9282</orcidid><orcidid>https://orcid.org/0000-0002-3670-9877</orcidid><orcidid>https://orcid.org/0000-0001-9026-5149</orcidid><orcidid>https://orcid.org/0000-0001-9134-6573</orcidid><orcidid>https://orcid.org/0000-0003-3036-1512</orcidid><orcidid>https://orcid.org/0000-0002-5577-2329</orcidid><orcidid>https://orcid.org/0000-0001-8040-8927</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Addictions Apoptosis chronic constriction injury chronic neuralgia Constrictions Drugs Hyperalgesia Inflammation Interleukin 1 Interleukin 10 Interleukin 6 Lipopolysaccharides Nerves Neuralgia paeoniflorin Pain perception Schwann cells Sciatic nerve Sciatica TLR4 protein TLR4/NF‐kB Toll-like receptors Tumor necrosis factor |
| title | Therapeutic effect of paeoniflorin on chronic constriction injury of the sciatic nerve via the inhibition of Schwann cell apoptosis |
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