Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients
Multiple myeloma results from the clonal proliferation of abnormal plasma cells (PCs) in the bone marrow (BM). In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome...
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| Veröffentlicht in: | International journal of molecular sciences 2022-07, Vol.23 (14), p.7530 |
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| creator | Dragoș, Mihaiela L Ivanov, Iuliu C Mențel, Mihaela Văcărean-Trandafir, Irina C Sireteanu, Adriana Titianu, Amalia A Dăscălescu, Angela S Stache, Alexandru B Jitaru, Daniela Gorgan, Dragoș L |
| description | Multiple myeloma results from the clonal proliferation of abnormal plasma cells (PCs) in the bone marrow (BM). In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome (detected by multiplex ligation-dependent probe amplification (MLPA)) to assess their impact on prognosis in newly diagnosed MM patients. Statistically significant results were obtained when different stages of PC maturation (classified based on CD19 and CD81 expression) were associated with CD117 expression and identified CNAs. In the intermediately differentiated PC group (CD19(-) CD81(+)), patients who didn't express CD117 had a lower median progression free survival (PFS) (
= 0.024). Moreover, within this group, patients with less than three adverse CNAs, which harbor CD117, had a better outcome with a PFS of more than 48 months compared with 19 months (
= 0.008). Considering all the results, our study suggested the need to integrate both the CD markers and copy number alterations to evaluate the prognosis of newly diagnosed multiple myeloma patients. |
| doi_str_mv | 10.3390/ijms23147530 |
| format | Article |
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= 0.024). Moreover, within this group, patients with less than three adverse CNAs, which harbor CD117, had a better outcome with a PFS of more than 48 months compared with 19 months (
= 0.008). Considering all the results, our study suggested the need to integrate both the CD markers and copy number alterations to evaluate the prognosis of newly diagnosed multiple myeloma patients.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms23147530</identifier><identifier>PMID: 35886877</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antigens ; Bone marrow ; Bone Marrow - metabolism ; CD19 antigen ; CD81 antigen ; Cell proliferation ; Cell surface ; Chromosomes ; Copy number ; DNA Copy Number Variations - genetics ; Flow Cytometry ; Genomes ; Hematology ; Humans ; Immunoglobulins ; Markers ; Medical prognosis ; Multiple myeloma ; Multiple Myeloma - diagnosis ; Multiple Myeloma - genetics ; Multiple Myeloma - metabolism ; Older people ; Plasma ; Plasma cells ; Prognosis ; Statistical analysis</subject><ispartof>International journal of molecular sciences, 2022-07, Vol.23 (14), p.7530</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-4e64c57c47805c63d2f8551c65af0d8a08d43e69d99e19c3ef70d252c2390cd63</citedby><cites>FETCH-LOGICAL-c342t-4e64c57c47805c63d2f8551c65af0d8a08d43e69d99e19c3ef70d252c2390cd63</cites><orcidid>0000-0001-5252-8075 ; 0000-0002-7329-5431 ; 0000-0001-6454-9092 ; 0000-0001-5372-9901 ; 0000-0002-0878-5055</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318311/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318311/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35886877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dragoș, Mihaiela L</creatorcontrib><creatorcontrib>Ivanov, Iuliu C</creatorcontrib><creatorcontrib>Mențel, Mihaela</creatorcontrib><creatorcontrib>Văcărean-Trandafir, Irina C</creatorcontrib><creatorcontrib>Sireteanu, Adriana</creatorcontrib><creatorcontrib>Titianu, Amalia A</creatorcontrib><creatorcontrib>Dăscălescu, Angela S</creatorcontrib><creatorcontrib>Stache, Alexandru B</creatorcontrib><creatorcontrib>Jitaru, Daniela</creatorcontrib><creatorcontrib>Gorgan, Dragoș L</creatorcontrib><title>Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Multiple myeloma results from the clonal proliferation of abnormal plasma cells (PCs) in the bone marrow (BM). In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome (detected by multiplex ligation-dependent probe amplification (MLPA)) to assess their impact on prognosis in newly diagnosed MM patients. Statistically significant results were obtained when different stages of PC maturation (classified based on CD19 and CD81 expression) were associated with CD117 expression and identified CNAs. In the intermediately differentiated PC group (CD19(-) CD81(+)), patients who didn't express CD117 had a lower median progression free survival (PFS) (
= 0.024). Moreover, within this group, patients with less than three adverse CNAs, which harbor CD117, had a better outcome with a PFS of more than 48 months compared with 19 months (
= 0.008). 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In this study, the cell surface expression markers (CD) on atypical PCs (detected by multiparametric flow cytometry (MFC)) were correlated with copy number alterations (CNAs) in the genome (detected by multiplex ligation-dependent probe amplification (MLPA)) to assess their impact on prognosis in newly diagnosed MM patients. Statistically significant results were obtained when different stages of PC maturation (classified based on CD19 and CD81 expression) were associated with CD117 expression and identified CNAs. In the intermediately differentiated PC group (CD19(-) CD81(+)), patients who didn't express CD117 had a lower median progression free survival (PFS) (
= 0.024). Moreover, within this group, patients with less than three adverse CNAs, which harbor CD117, had a better outcome with a PFS of more than 48 months compared with 19 months (
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| subjects | Antigens Bone marrow Bone Marrow - metabolism CD19 antigen CD81 antigen Cell proliferation Cell surface Chromosomes Copy number DNA Copy Number Variations - genetics Flow Cytometry Genomes Hematology Humans Immunoglobulins Markers Medical prognosis Multiple myeloma Multiple Myeloma - diagnosis Multiple Myeloma - genetics Multiple Myeloma - metabolism Older people Plasma Plasma cells Prognosis Statistical analysis |
| title | Prognostic Value of Association of Copy Number Alterations and Cell-Surface Expression Markers in Newly Diagnosed Multiple Myeloma Patients |
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