Experimental Models for Cold Exposure of Muscle in vitroand in vivo

Experimental Models for Cold Exposure of Muscle in vitroand in vivo

Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of cold exposure in muscle, and since adequate methods we...

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Veröffentlicht in:Bio-protocol 2022-07, Vol.12 (13), p.e4461-e4461
Hauptverfasser: Schjølberg, Tiril, Asoawe, Lucia, Krapf, Solveig, Rustan, Arild C, Thoresen, Hege G, Haugen, Fred
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creator Schjølberg, Tiril
Asoawe, Lucia
Krapf, Solveig
Rustan, Arild C
Thoresen, Hege G
Haugen, Fred
description Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of cold exposure in muscle, and since adequate methods were missing, we established two experimental cold exposure models: 1) In vitro exposure to cold (18°C) or control temperature (37°C) of cultured human skeletal muscle cells (myotubes); and 2) unilateral cold exposure of hind limb skeletal muscle in anesthetized rats (intramuscular temperature 18°C), with contralateral control (37°C). This methodology enables studies of muscle responses to local cold exposures at the level of gene expression, but also other molecular outcomes. Graphical abstract:
doi_str_mv 10.21769/BioProtoc.4461
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In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of cold exposure in muscle, and since adequate methods were missing, we established two experimental cold exposure models: 1) In vitro exposure to cold (18°C) or control temperature (37°C) of cultured human skeletal muscle cells (myotubes); and 2) unilateral cold exposure of hind limb skeletal muscle in anesthetized rats (intramuscular temperature 18°C), with contralateral control (37°C). This methodology enables studies of muscle responses to local cold exposures at the level of gene expression, but also other molecular outcomes. 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title Experimental Models for Cold Exposure of Muscle in vitroand in vivo
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