Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7
Background. Combination of natural products with chemically synthesised biomaterials as cancer therapy has attracted great interest lately. Hence, this study is aimed at investigating the combined effects of goniothalamin and bioactive glass 45S5 (GTN-BG) and evaluating their anticancer properties o...
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| creator | Bakar, Siti Aishah Abu Ali, Abdul Manaf Noor, Siti Noor Fazliah Mohd Hamid, Shahrul Bariyah Sahul Azhar, Nur Asna Mohamad, Noor Muzamil Ahmad, Nor Hazwani |
| description | Background. Combination of natural products with chemically synthesised biomaterials as cancer therapy has attracted great interest lately. Hence, this study is aimed at investigating the combined effects of goniothalamin and bioactive glass 45S5 (GTN-BG) and evaluating their anticancer properties on human breast cancer cells MCF-7. Methods. The BG 45S5 was prepared using the sol-gel process followed by characterisation using PSA, BET, SEM/EDS, XRD, and FTIR. The effects of GTN-BG on the proliferation of MCF-7 were assessed by MTT, PrestoBlue, and scratch wound assays. The cell cycle analysis, Annexin-FITC assay, and activation of caspase-3/7, caspase-8, and caspase-9 assays were determined to further explore its mechanism of action. Results. The synthesised BG 45S5 was classified as a fine powder, having a rough surface, and contains mesopores of 12.6 nm. EDS analysis revealed that silica and calcium elements are the primary components of BG powders. Both crystalline and amorphous structures were detected with 73% and 27% similarity to Na2Ca2(Si2O7) and hydroxyapatite, respectively. The combination of GTN-BG was more potent than GTN in inhibiting the proliferation of MCF-7 cells. G0/G1 and G2/M phases of the cell cycle were arrested by GTN and GTN-BG. The percentage of viable cells in GTN-BG treatment was significantly lower than that in GTN. In terms of activation of initiator caspases for both extrinsic and intrinsic apoptosis pathways, caspase-8 and caspase-9 were found more effective in response to GTN-BG than GTN. Conclusion. The anticancer effect of GTN in MCF-7 cells was improved when combined with BG. The findings provide significant insight into the mechanism of GTN-BG against MCF-7 cells, which can potentially be used as a novel anticancer therapeutic approach. |
| doi_str_mv | 10.1155/2022/5653136 |
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| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9303150</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A711331694</galeid><sourcerecordid>A711331694</sourcerecordid><originalsourceid>FETCH-LOGICAL-c433t-4babc817bf9f1f17abe6c6ca9ab3df3f9968c353d1abe50b12c23cd7877fafb43</originalsourceid><addsrcrecordid>eNp9ks1u1DAUhSMEolXpjjWyxAYJQuM4TuINUhratFIRi8LaunHsxpXHHuxkqnkvHrBOZxh-FnjjK91P5_jIJ0le4-wjxpSe5Vmen9GSEkzKZ8lxTnCRlrjAzw8zIUfJaQj3WTw1LjNWvkyOCK2rvCbsOPnZulWvLUzaWeQU6pzVbhrBwEpbBHZAt86knTTpZ-n1Rg7oXDsQUxxRZyAEVNBbii7sCFbIgDrvHqYRXdtR93pyfouaBdbTFm00oGbt1pMLOkRimMWT62LSSmNQuxVGosZ7GSYU3c-9hDi1i7J_QgL60l6m1avkhQIT5On-Pkm-X158a6_Sm6_dddvcpKIgZEqLHnpR46pXTGGFK-hlKUoBDHoyKKIYK2tBKBlw3NCsx7nIiRiquqoUqL4gJ8mnne567ldyENJOHgxfe70Cv-UONP97Y_XI79yGM5IRTLMo8G4v4N2POcbiKx1EDAJWujnwvGRFkWc0ZxF9-w9672ZvY7yFIrRkjNLf1B0YybVVLvqKRZQ3FY6fjaNipD7sKOFdCF6qw5Nxxpfe8KU3fN-biL_5M-YB_tWSCLzfAaO2Azzo_8s9AmrDy4Y</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2693569955</pqid></control><display><type>article</type><title>Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7</title><source>MEDLINE</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Bakar, Siti Aishah Abu ; Ali, Abdul Manaf ; Noor, Siti Noor Fazliah Mohd ; Hamid, Shahrul Bariyah Sahul ; Azhar, Nur Asna ; Mohamad, Noor Muzamil ; Ahmad, Nor Hazwani</creator><contributor>Galani, Vasiliki</contributor><creatorcontrib>Bakar, Siti Aishah Abu ; Ali, Abdul Manaf ; Noor, Siti Noor Fazliah Mohd ; Hamid, Shahrul Bariyah Sahul ; Azhar, Nur Asna ; Mohamad, Noor Muzamil ; Ahmad, Nor Hazwani ; Galani, Vasiliki</creatorcontrib><description>Background. Combination of natural products with chemically synthesised biomaterials as cancer therapy has attracted great interest lately. Hence, this study is aimed at investigating the combined effects of goniothalamin and bioactive glass 45S5 (GTN-BG) and evaluating their anticancer properties on human breast cancer cells MCF-7. Methods. The BG 45S5 was prepared using the sol-gel process followed by characterisation using PSA, BET, SEM/EDS, XRD, and FTIR. The effects of GTN-BG on the proliferation of MCF-7 were assessed by MTT, PrestoBlue, and scratch wound assays. The cell cycle analysis, Annexin-FITC assay, and activation of caspase-3/7, caspase-8, and caspase-9 assays were determined to further explore its mechanism of action. Results. The synthesised BG 45S5 was classified as a fine powder, having a rough surface, and contains mesopores of 12.6 nm. EDS analysis revealed that silica and calcium elements are the primary components of BG powders. Both crystalline and amorphous structures were detected with 73% and 27% similarity to Na2Ca2(Si2O7) and hydroxyapatite, respectively. The combination of GTN-BG was more potent than GTN in inhibiting the proliferation of MCF-7 cells. G0/G1 and G2/M phases of the cell cycle were arrested by GTN and GTN-BG. The percentage of viable cells in GTN-BG treatment was significantly lower than that in GTN. In terms of activation of initiator caspases for both extrinsic and intrinsic apoptosis pathways, caspase-8 and caspase-9 were found more effective in response to GTN-BG than GTN. Conclusion. The anticancer effect of GTN in MCF-7 cells was improved when combined with BG. The findings provide significant insight into the mechanism of GTN-BG against MCF-7 cells, which can potentially be used as a novel anticancer therapeutic approach.</description><identifier>ISSN: 2314-6133</identifier><identifier>ISSN: 2314-6141</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2022/5653136</identifier><identifier>PMID: 35872839</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Anticancer properties ; Apoptosis ; Assaying ; Bioglass ; Biological activity ; Biomaterials ; Biomedical materials ; Breast cancer ; Breast Neoplasms - drug therapy ; Cancer therapies ; Care and treatment ; Caspase 8 ; Caspase 9 ; Caspase-3 ; Cell cycle ; Cell Cycle Checkpoints ; Cell growth ; Cell Proliferation ; Ceramics ; Chemotherapy ; Female ; Fourier transforms ; Glass ; Health aspects ; Humans ; Hydroxyapatite ; Lactones ; MCF-7 Cells ; Natural products ; Nitrates ; Particle size ; Pharmacology, Experimental ; Pyrones ; Silica ; Sol-gel processes ; Testing</subject><ispartof>BioMed research international, 2022, Vol.2022 (1), p.5653136</ispartof><rights>Copyright © 2022 Siti Aishah Abu Bakar et al.</rights><rights>COPYRIGHT 2022 John Wiley & Sons, Inc.</rights><rights>Copyright © 2022 Siti Aishah Abu Bakar et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2022 Siti Aishah Abu Bakar et al. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c433t-4babc817bf9f1f17abe6c6ca9ab3df3f9968c353d1abe50b12c23cd7877fafb43</cites><orcidid>0000-0002-2843-026X ; 0000-0001-7353-2495</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303150/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303150/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35872839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Galani, Vasiliki</contributor><creatorcontrib>Bakar, Siti Aishah Abu</creatorcontrib><creatorcontrib>Ali, Abdul Manaf</creatorcontrib><creatorcontrib>Noor, Siti Noor Fazliah Mohd</creatorcontrib><creatorcontrib>Hamid, Shahrul Bariyah Sahul</creatorcontrib><creatorcontrib>Azhar, Nur Asna</creatorcontrib><creatorcontrib>Mohamad, Noor Muzamil</creatorcontrib><creatorcontrib>Ahmad, Nor Hazwani</creatorcontrib><title>Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Background. Combination of natural products with chemically synthesised biomaterials as cancer therapy has attracted great interest lately. Hence, this study is aimed at investigating the combined effects of goniothalamin and bioactive glass 45S5 (GTN-BG) and evaluating their anticancer properties on human breast cancer cells MCF-7. Methods. The BG 45S5 was prepared using the sol-gel process followed by characterisation using PSA, BET, SEM/EDS, XRD, and FTIR. The effects of GTN-BG on the proliferation of MCF-7 were assessed by MTT, PrestoBlue, and scratch wound assays. The cell cycle analysis, Annexin-FITC assay, and activation of caspase-3/7, caspase-8, and caspase-9 assays were determined to further explore its mechanism of action. Results. The synthesised BG 45S5 was classified as a fine powder, having a rough surface, and contains mesopores of 12.6 nm. EDS analysis revealed that silica and calcium elements are the primary components of BG powders. Both crystalline and amorphous structures were detected with 73% and 27% similarity to Na2Ca2(Si2O7) and hydroxyapatite, respectively. The combination of GTN-BG was more potent than GTN in inhibiting the proliferation of MCF-7 cells. G0/G1 and G2/M phases of the cell cycle were arrested by GTN and GTN-BG. The percentage of viable cells in GTN-BG treatment was significantly lower than that in GTN. In terms of activation of initiator caspases for both extrinsic and intrinsic apoptosis pathways, caspase-8 and caspase-9 were found more effective in response to GTN-BG than GTN. Conclusion. The anticancer effect of GTN in MCF-7 cells was improved when combined with BG. The findings provide significant insight into the mechanism of GTN-BG against MCF-7 cells, which can potentially be used as a novel anticancer therapeutic approach.</description><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Assaying</subject><subject>Bioglass</subject><subject>Biological activity</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Cancer therapies</subject><subject>Care and treatment</subject><subject>Caspase 8</subject><subject>Caspase 9</subject><subject>Caspase-3</subject><subject>Cell cycle</subject><subject>Cell Cycle Checkpoints</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Ceramics</subject><subject>Chemotherapy</subject><subject>Female</subject><subject>Fourier transforms</subject><subject>Glass</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hydroxyapatite</subject><subject>Lactones</subject><subject>MCF-7 Cells</subject><subject>Natural products</subject><subject>Nitrates</subject><subject>Particle size</subject><subject>Pharmacology, Experimental</subject><subject>Pyrones</subject><subject>Silica</subject><subject>Sol-gel processes</subject><subject>Testing</subject><issn>2314-6133</issn><issn>2314-6141</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9ks1u1DAUhSMEolXpjjWyxAYJQuM4TuINUhratFIRi8LaunHsxpXHHuxkqnkvHrBOZxh-FnjjK91P5_jIJ0le4-wjxpSe5Vmen9GSEkzKZ8lxTnCRlrjAzw8zIUfJaQj3WTw1LjNWvkyOCK2rvCbsOPnZulWvLUzaWeQU6pzVbhrBwEpbBHZAt86knTTpZ-n1Rg7oXDsQUxxRZyAEVNBbii7sCFbIgDrvHqYRXdtR93pyfouaBdbTFm00oGbt1pMLOkRimMWT62LSSmNQuxVGosZ7GSYU3c-9hDi1i7J_QgL60l6m1avkhQIT5On-Pkm-X158a6_Sm6_dddvcpKIgZEqLHnpR46pXTGGFK-hlKUoBDHoyKKIYK2tBKBlw3NCsx7nIiRiquqoUqL4gJ8mnne567ldyENJOHgxfe70Cv-UONP97Y_XI79yGM5IRTLMo8G4v4N2POcbiKx1EDAJWujnwvGRFkWc0ZxF9-w9672ZvY7yFIrRkjNLf1B0YybVVLvqKRZQ3FY6fjaNipD7sKOFdCF6qw5Nxxpfe8KU3fN-biL_5M-YB_tWSCLzfAaO2Azzo_8s9AmrDy4Y</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Bakar, Siti Aishah Abu</creator><creator>Ali, Abdul Manaf</creator><creator>Noor, Siti Noor Fazliah Mohd</creator><creator>Hamid, Shahrul Bariyah Sahul</creator><creator>Azhar, Nur Asna</creator><creator>Mohamad, Noor Muzamil</creator><creator>Ahmad, Nor Hazwani</creator><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2843-026X</orcidid><orcidid>https://orcid.org/0000-0001-7353-2495</orcidid></search><sort><creationdate>2022</creationdate><title>Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7</title><author>Bakar, Siti Aishah Abu ; Ali, Abdul Manaf ; Noor, Siti Noor Fazliah Mohd ; Hamid, Shahrul Bariyah Sahul ; Azhar, Nur Asna ; Mohamad, Noor Muzamil ; Ahmad, Nor Hazwani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-4babc817bf9f1f17abe6c6ca9ab3df3f9968c353d1abe50b12c23cd7877fafb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Assaying</topic><topic>Bioglass</topic><topic>Biological activity</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Cancer therapies</topic><topic>Care and treatment</topic><topic>Caspase 8</topic><topic>Caspase 9</topic><topic>Caspase-3</topic><topic>Cell cycle</topic><topic>Cell Cycle Checkpoints</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Ceramics</topic><topic>Chemotherapy</topic><topic>Female</topic><topic>Fourier transforms</topic><topic>Glass</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Hydroxyapatite</topic><topic>Lactones</topic><topic>MCF-7 Cells</topic><topic>Natural products</topic><topic>Nitrates</topic><topic>Particle size</topic><topic>Pharmacology, Experimental</topic><topic>Pyrones</topic><topic>Silica</topic><topic>Sol-gel processes</topic><topic>Testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bakar, Siti Aishah Abu</creatorcontrib><creatorcontrib>Ali, Abdul Manaf</creatorcontrib><creatorcontrib>Noor, Siti Noor Fazliah Mohd</creatorcontrib><creatorcontrib>Hamid, Shahrul Bariyah Sahul</creatorcontrib><creatorcontrib>Azhar, Nur Asna</creatorcontrib><creatorcontrib>Mohamad, Noor Muzamil</creatorcontrib><creatorcontrib>Ahmad, Nor Hazwani</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bakar, Siti Aishah Abu</au><au>Ali, Abdul Manaf</au><au>Noor, Siti Noor Fazliah Mohd</au><au>Hamid, Shahrul Bariyah Sahul</au><au>Azhar, Nur Asna</au><au>Mohamad, Noor Muzamil</au><au>Ahmad, Nor Hazwani</au><au>Galani, Vasiliki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2022</date><risdate>2022</risdate><volume>2022</volume><issue>1</issue><spage>5653136</spage><pages>5653136-</pages><issn>2314-6133</issn><issn>2314-6141</issn><eissn>2314-6141</eissn><abstract>Background. Combination of natural products with chemically synthesised biomaterials as cancer therapy has attracted great interest lately. Hence, this study is aimed at investigating the combined effects of goniothalamin and bioactive glass 45S5 (GTN-BG) and evaluating their anticancer properties on human breast cancer cells MCF-7. Methods. The BG 45S5 was prepared using the sol-gel process followed by characterisation using PSA, BET, SEM/EDS, XRD, and FTIR. The effects of GTN-BG on the proliferation of MCF-7 were assessed by MTT, PrestoBlue, and scratch wound assays. The cell cycle analysis, Annexin-FITC assay, and activation of caspase-3/7, caspase-8, and caspase-9 assays were determined to further explore its mechanism of action. Results. The synthesised BG 45S5 was classified as a fine powder, having a rough surface, and contains mesopores of 12.6 nm. EDS analysis revealed that silica and calcium elements are the primary components of BG powders. Both crystalline and amorphous structures were detected with 73% and 27% similarity to Na2Ca2(Si2O7) and hydroxyapatite, respectively. The combination of GTN-BG was more potent than GTN in inhibiting the proliferation of MCF-7 cells. G0/G1 and G2/M phases of the cell cycle were arrested by GTN and GTN-BG. The percentage of viable cells in GTN-BG treatment was significantly lower than that in GTN. In terms of activation of initiator caspases for both extrinsic and intrinsic apoptosis pathways, caspase-8 and caspase-9 were found more effective in response to GTN-BG than GTN. Conclusion. The anticancer effect of GTN in MCF-7 cells was improved when combined with BG. The findings provide significant insight into the mechanism of GTN-BG against MCF-7 cells, which can potentially be used as a novel anticancer therapeutic approach.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>35872839</pmid><doi>10.1155/2022/5653136</doi><orcidid>https://orcid.org/0000-0002-2843-026X</orcidid><orcidid>https://orcid.org/0000-0001-7353-2495</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anticancer properties Apoptosis Assaying Bioglass Biological activity Biomaterials Biomedical materials Breast cancer Breast Neoplasms - drug therapy Cancer therapies Care and treatment Caspase 8 Caspase 9 Caspase-3 Cell cycle Cell Cycle Checkpoints Cell growth Cell Proliferation Ceramics Chemotherapy Female Fourier transforms Glass Health aspects Humans Hydroxyapatite Lactones MCF-7 Cells Natural products Nitrates Particle size Pharmacology, Experimental Pyrones Silica Sol-gel processes Testing |
| title | Combination of Goniothalamin and Sol-Gel-Derived Bioactive Glass 45S5 Enhances Growth Inhibitory Activity via Apoptosis Induction and Cell Cycle Arrest in Breast Cancer Cells MCF-7 |
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