Digital holography-based 3D particle localization for single-molecule tweezer techniques

We present a three-dimensional (3D) imaging technique for the fast tracking of microscopic objects in a fluid environment. Our technique couples digital holographic microscopy with three-dimensional localization via parabolic masking. Compared with existing approaches, our method reconstructs 3D vol...

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Veröffentlicht in:	Biophysical journal 2022-07, Vol.121 (13), p.2538-2549
Hauptverfasser:	Flewellen, James L., Minoughan, Sophie, Garcia, Isabel Llorente, Tolar, Pavel
Format:	Artikel
Sprache:	eng
Schlagworte:	Holography - methods Imaging, Three-Dimensional - methods Lab-On-A-Chip Devices Microscopy - methods Single Molecule Imaging
Online-Zugang:	Volltext
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