Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts
Background There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation. Met...
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| Veröffentlicht in: | Artificial organs 2022-02, Vol.46 (2), p.246-258 |
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| creator | Suylen, Vincent Vandendriessche, Katrien Neyrinck, Arne Nijhuis, Foppe Plaats, Arjan Verbeken, Erik K. Vermeersch, Pieter Meyns, Bart Mariani, Massimo A. Rega, Filip Erasmus, Michiel E. |
| description | Background
There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.
Methods
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.
Results
For both groups, in vivo baseline and post‐Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (p = 0.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30 min up to 90 min of reperfusion. Contractility was superior in MP during the first hour of evaluation.
Conclusion
We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization.
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness. We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. |
| doi_str_mv | 10.1111/aor.14085 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9298357</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2580933014</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4435-53a05740892d81da8b4745556107c3ec48ecd50a611f7d0e449d509df3b352993</originalsourceid><addsrcrecordid>eNp1kV1rFDEUhoModq1e-Ack4I1eTJtMPmZyI5TiFxQWRMG7cDY5s5syM1mTTHX_valbiwrmJic5Tx4OeQl5ztkZr-scYjrjkvXqAVlx1aqGKyMfkhXjmjVKy68n5EnO14yxTjL9mJwIqYXQnV6RtP5x2OIMBT2dwO3CjHSPaVhyiDOFQlOMEy041UsoS0IKmULtjAVTfRZukA4x0Vxq7aiLo691TLBFGma6j8ndKn2cK7RDSCU_JY8GGDM-u9tPyZd3bz9ffmiu1u8_Xl5cNU5KoRolgKk6b29a33MP_UZ2UimlOeucQCd7dF4x0JwPnWcopalH4wexEao1RpySN0fvftlM6B3OJcFo9ylMkA42QrB_d-aws9t4Y01reqG6Knh1J0jx24K52Clkh-MIM8Yl21b1zAjBuKzoy3_Q67jU_xkrpXkvtWZSVer1kXIp5pxwuB-GM3ubpK1J2l9JVvbFn9Pfk7-jq8D5EfgeRjz832Qv1p-Oyp-hM6lw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2618466045</pqid></control><display><type>article</type><title>Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Suylen, Vincent ; Vandendriessche, Katrien ; Neyrinck, Arne ; Nijhuis, Foppe ; Plaats, Arjan ; Verbeken, Erik K. ; Vermeersch, Pieter ; Meyns, Bart ; Mariani, Massimo A. ; Rega, Filip ; Erasmus, Michiel E.</creator><creatorcontrib>Suylen, Vincent ; Vandendriessche, Katrien ; Neyrinck, Arne ; Nijhuis, Foppe ; Plaats, Arjan ; Verbeken, Erik K. ; Vermeersch, Pieter ; Meyns, Bart ; Mariani, Massimo A. ; Rega, Filip ; Erasmus, Michiel E.</creatorcontrib><description>Background
There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.
Methods
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.
Results
For both groups, in vivo baseline and post‐Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (p = 0.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30 min up to 90 min of reperfusion. Contractility was superior in MP during the first hour of evaluation.
Conclusion
We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization.
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness. We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation.</description><identifier>ISSN: 0160-564X</identifier><identifier>EISSN: 1525-1594</identifier><identifier>DOI: 10.1111/aor.14085</identifier><identifier>PMID: 34633676</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Biopsy ; Cold storage ; Cold Temperature ; Cryopreservation ; Evaluation ; ex situ heart perfusion ; Female ; Heart - physiology ; Heart Arrest, Induced ; Heart Transplantation ; Histology ; Hypothermia ; Lactic acid ; Lactic Acid - metabolism ; machine perfusion ; Main Text ; Muscle contraction ; Optimization ; Organ Preservation - instrumentation ; Organ Preservation - methods ; Oxygen - metabolism ; Oxygen consumption ; Oxygenation ; Perfusion ; Perfusion - methods ; preservation ; Reperfusion ; Room temperature ; Stiffness ; Swine ; temperature ; Transplantation</subject><ispartof>Artificial organs, 2022-02, Vol.46 (2), p.246-258</ispartof><rights>2021 The Authors. published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.</rights><rights>2021 The Authors. Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.</rights><rights>2021. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4435-53a05740892d81da8b4745556107c3ec48ecd50a611f7d0e449d509df3b352993</citedby><cites>FETCH-LOGICAL-c4435-53a05740892d81da8b4745556107c3ec48ecd50a611f7d0e449d509df3b352993</cites><orcidid>0000-0002-8428-2302 ; 0000-0002-5405-1343</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faor.14085$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faor.14085$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34633676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suylen, Vincent</creatorcontrib><creatorcontrib>Vandendriessche, Katrien</creatorcontrib><creatorcontrib>Neyrinck, Arne</creatorcontrib><creatorcontrib>Nijhuis, Foppe</creatorcontrib><creatorcontrib>Plaats, Arjan</creatorcontrib><creatorcontrib>Verbeken, Erik K.</creatorcontrib><creatorcontrib>Vermeersch, Pieter</creatorcontrib><creatorcontrib>Meyns, Bart</creatorcontrib><creatorcontrib>Mariani, Massimo A.</creatorcontrib><creatorcontrib>Rega, Filip</creatorcontrib><creatorcontrib>Erasmus, Michiel E.</creatorcontrib><title>Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts</title><title>Artificial organs</title><addtitle>Artif Organs</addtitle><description>Background
There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.
Methods
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.
Results
For both groups, in vivo baseline and post‐Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (p = 0.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30 min up to 90 min of reperfusion. Contractility was superior in MP during the first hour of evaluation.
Conclusion
We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization.
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness. We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation.</description><subject>Animals</subject><subject>Biopsy</subject><subject>Cold storage</subject><subject>Cold Temperature</subject><subject>Cryopreservation</subject><subject>Evaluation</subject><subject>ex situ heart perfusion</subject><subject>Female</subject><subject>Heart - physiology</subject><subject>Heart Arrest, Induced</subject><subject>Heart Transplantation</subject><subject>Histology</subject><subject>Hypothermia</subject><subject>Lactic acid</subject><subject>Lactic Acid - metabolism</subject><subject>machine perfusion</subject><subject>Main Text</subject><subject>Muscle contraction</subject><subject>Optimization</subject><subject>Organ Preservation - instrumentation</subject><subject>Organ Preservation - methods</subject><subject>Oxygen - metabolism</subject><subject>Oxygen consumption</subject><subject>Oxygenation</subject><subject>Perfusion</subject><subject>Perfusion - methods</subject><subject>preservation</subject><subject>Reperfusion</subject><subject>Room temperature</subject><subject>Stiffness</subject><subject>Swine</subject><subject>temperature</subject><subject>Transplantation</subject><issn>0160-564X</issn><issn>1525-1594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kV1rFDEUhoModq1e-Ack4I1eTJtMPmZyI5TiFxQWRMG7cDY5s5syM1mTTHX_valbiwrmJic5Tx4OeQl5ztkZr-scYjrjkvXqAVlx1aqGKyMfkhXjmjVKy68n5EnO14yxTjL9mJwIqYXQnV6RtP5x2OIMBT2dwO3CjHSPaVhyiDOFQlOMEy041UsoS0IKmULtjAVTfRZukA4x0Vxq7aiLo691TLBFGma6j8ndKn2cK7RDSCU_JY8GGDM-u9tPyZd3bz9ffmiu1u8_Xl5cNU5KoRolgKk6b29a33MP_UZ2UimlOeucQCd7dF4x0JwPnWcopalH4wexEao1RpySN0fvftlM6B3OJcFo9ylMkA42QrB_d-aws9t4Y01reqG6Knh1J0jx24K52Clkh-MIM8Yl21b1zAjBuKzoy3_Q67jU_xkrpXkvtWZSVer1kXIp5pxwuB-GM3ubpK1J2l9JVvbFn9Pfk7-jq8D5EfgeRjz832Qv1p-Oyp-hM6lw</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Suylen, Vincent</creator><creator>Vandendriessche, Katrien</creator><creator>Neyrinck, Arne</creator><creator>Nijhuis, Foppe</creator><creator>Plaats, Arjan</creator><creator>Verbeken, Erik K.</creator><creator>Vermeersch, Pieter</creator><creator>Meyns, Bart</creator><creator>Mariani, Massimo A.</creator><creator>Rega, Filip</creator><creator>Erasmus, Michiel E.</creator><general>Wiley Subscription Services, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8428-2302</orcidid><orcidid>https://orcid.org/0000-0002-5405-1343</orcidid></search><sort><creationdate>202202</creationdate><title>Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts</title><author>Suylen, Vincent ; Vandendriessche, Katrien ; Neyrinck, Arne ; Nijhuis, Foppe ; Plaats, Arjan ; Verbeken, Erik K. ; Vermeersch, Pieter ; Meyns, Bart ; Mariani, Massimo A. ; Rega, Filip ; Erasmus, Michiel E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4435-53a05740892d81da8b4745556107c3ec48ecd50a611f7d0e449d509df3b352993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Biopsy</topic><topic>Cold storage</topic><topic>Cold Temperature</topic><topic>Cryopreservation</topic><topic>Evaluation</topic><topic>ex situ heart perfusion</topic><topic>Female</topic><topic>Heart - physiology</topic><topic>Heart Arrest, Induced</topic><topic>Heart Transplantation</topic><topic>Histology</topic><topic>Hypothermia</topic><topic>Lactic acid</topic><topic>Lactic Acid - metabolism</topic><topic>machine perfusion</topic><topic>Main Text</topic><topic>Muscle contraction</topic><topic>Optimization</topic><topic>Organ Preservation - instrumentation</topic><topic>Organ Preservation - methods</topic><topic>Oxygen - metabolism</topic><topic>Oxygen consumption</topic><topic>Oxygenation</topic><topic>Perfusion</topic><topic>Perfusion - methods</topic><topic>preservation</topic><topic>Reperfusion</topic><topic>Room temperature</topic><topic>Stiffness</topic><topic>Swine</topic><topic>temperature</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suylen, Vincent</creatorcontrib><creatorcontrib>Vandendriessche, Katrien</creatorcontrib><creatorcontrib>Neyrinck, Arne</creatorcontrib><creatorcontrib>Nijhuis, Foppe</creatorcontrib><creatorcontrib>Plaats, Arjan</creatorcontrib><creatorcontrib>Verbeken, Erik K.</creatorcontrib><creatorcontrib>Vermeersch, Pieter</creatorcontrib><creatorcontrib>Meyns, Bart</creatorcontrib><creatorcontrib>Mariani, Massimo A.</creatorcontrib><creatorcontrib>Rega, Filip</creatorcontrib><creatorcontrib>Erasmus, Michiel E.</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suylen, Vincent</au><au>Vandendriessche, Katrien</au><au>Neyrinck, Arne</au><au>Nijhuis, Foppe</au><au>Plaats, Arjan</au><au>Verbeken, Erik K.</au><au>Vermeersch, Pieter</au><au>Meyns, Bart</au><au>Mariani, Massimo A.</au><au>Rega, Filip</au><au>Erasmus, Michiel E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts</atitle><jtitle>Artificial organs</jtitle><addtitle>Artif Organs</addtitle><date>2022-02</date><risdate>2022</risdate><volume>46</volume><issue>2</issue><spage>246</spage><epage>258</epage><pages>246-258</pages><issn>0160-564X</issn><eissn>1525-1594</eissn><abstract>Background
There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.
Methods
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.
Results
For both groups, in vivo baseline and post‐Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (p = 0.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30 min up to 90 min of reperfusion. Contractility was superior in MP during the first hour of evaluation.
Conclusion
We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization.
Cardioplegia‐arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, n = 5) or machine perfusion using an oxygenated acellular perfusate at 21°C (MP, n = 5). Subsequently, all grafts were evaluated using the Langendorff method for 120 min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness. We conclude that the initial functional outcome of MP‐preserved hearts was transiently superior compared to CS, with no histological injury post‐Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34633676</pmid><doi>10.1111/aor.14085</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-8428-2302</orcidid><orcidid>https://orcid.org/0000-0002-5405-1343</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Artificial organs, 2022-02, Vol.46 (2), p.246-258 |
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| subjects | Animals Biopsy Cold storage Cold Temperature Cryopreservation Evaluation ex situ heart perfusion Female Heart - physiology Heart Arrest, Induced Heart Transplantation Histology Hypothermia Lactic acid Lactic Acid - metabolism machine perfusion Main Text Muscle contraction Optimization Organ Preservation - instrumentation Organ Preservation - methods Oxygen - metabolism Oxygen consumption Oxygenation Perfusion Perfusion - methods preservation Reperfusion Room temperature Stiffness Swine temperature Transplantation |
| title | Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts |
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