Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever
For the last fifty years, Dengue has been one of the most common mosquito-borne arboviral infections which has spread over the tropical and subtropical world. Divya-Denguenil-Vati (DNV) has been formulated by blending five specific herbs for effective resolution of Dengue fever. In the present study...
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| Veröffentlicht in: | Chromatographia 2022, Vol.85 (9), p.831-850 |
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| creator | Balkrishna, Acharya Joshi, Monali Tomer, Meenu Verma, Sudeep Gujral, Seema Mulay, Vallabh Prakash Srivastava, Jyotish Varshney, Anurag |
| description | For the last fifty years, Dengue has been one of the most common mosquito-borne arboviral infections which has spread over the tropical and subtropical world. Divya-Denguenil-Vati (DNV) has been formulated by blending five specific herbs for effective resolution of Dengue fever. In the present study, we aimed to identify, develop, validate, and standardize methods for Divya-Denguenil-Vati (DNV), on UHPLC and HPTLC analytical platforms, with rapid, sensitive, accurate and rugged attributes. At first, 97 phyto-constituents were identified by UPLC/MS-QToF in Divya-Denguenil-Vati. UHPLC method was then developed and validated for simultaneous determination of gallic acid, 5-HMF, protocatechuic acid, magnoflorine, methyl gallate, berberine, rutin, ellagic acid, β-ecdysone and rosmarinic acid in DNV. Four selected markers, gallic acid, rosmarinic acid, magnoflorine and rutin were further developed and validated on HPTLC. Analytical processes were validated as per ICH Q2 (R1) guidelines; and were found linear (
r
2
> 0.99), sensitive, precise (%RSD |
| doi_str_mv | 10.1007/s10337-022-04183-7 |
| format | Article |
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r
2
> 0.99), sensitive, precise (%RSD < 5%), and accurate, as indicated by high recovery values (88–105%). The limit of detection and quantification were also established for these phyto-metabolites, with their respective RSDs within 5% limits. Finally, these validated methods were employed to test twenty six different commercial batches of DNV. The quality, reproducibility and consistency of DNV have been well established using these developed and reliable analytical tools. These analytical strategies successfully set a path forward for robust quality evaluation and standardization of Divya-Denguenil-Vati, and other related herbal formulations.</description><identifier>ISSN: 0009-5893</identifier><identifier>EISSN: 1612-1112</identifier><identifier>DOI: 10.1007/s10337-022-04183-7</identifier><identifier>PMID: 35875831</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Analytical Chemistry ; Chemistry ; Chemistry and Materials Science ; Chromatography ; Dengue fever ; Formulations ; Gallic acid ; Laboratory Medicine ; Mathematical analysis ; Metabolites ; Original ; Pharmacy ; Proteomics ; Quality assessment ; Rosmarinic acid ; Standardization</subject><ispartof>Chromatographia, 2022, Vol.85 (9), p.831-850</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-dae813dc2791afc68e2aa8d9e58553c92bdeaf27e1df112a5cdaa9e097858c433</citedby><cites>FETCH-LOGICAL-c381t-dae813dc2791afc68e2aa8d9e58553c92bdeaf27e1df112a5cdaa9e097858c433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10337-022-04183-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10337-022-04183-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Balkrishna, Acharya</creatorcontrib><creatorcontrib>Joshi, Monali</creatorcontrib><creatorcontrib>Tomer, Meenu</creatorcontrib><creatorcontrib>Verma, Sudeep</creatorcontrib><creatorcontrib>Gujral, Seema</creatorcontrib><creatorcontrib>Mulay, Vallabh Prakash</creatorcontrib><creatorcontrib>Srivastava, Jyotish</creatorcontrib><creatorcontrib>Varshney, Anurag</creatorcontrib><title>Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever</title><title>Chromatographia</title><addtitle>Chromatographia</addtitle><description>For the last fifty years, Dengue has been one of the most common mosquito-borne arboviral infections which has spread over the tropical and subtropical world. Divya-Denguenil-Vati (DNV) has been formulated by blending five specific herbs for effective resolution of Dengue fever. In the present study, we aimed to identify, develop, validate, and standardize methods for Divya-Denguenil-Vati (DNV), on UHPLC and HPTLC analytical platforms, with rapid, sensitive, accurate and rugged attributes. At first, 97 phyto-constituents were identified by UPLC/MS-QToF in Divya-Denguenil-Vati. UHPLC method was then developed and validated for simultaneous determination of gallic acid, 5-HMF, protocatechuic acid, magnoflorine, methyl gallate, berberine, rutin, ellagic acid, β-ecdysone and rosmarinic acid in DNV. Four selected markers, gallic acid, rosmarinic acid, magnoflorine and rutin were further developed and validated on HPTLC. Analytical processes were validated as per ICH Q2 (R1) guidelines; and were found linear (
r
2
> 0.99), sensitive, precise (%RSD < 5%), and accurate, as indicated by high recovery values (88–105%). The limit of detection and quantification were also established for these phyto-metabolites, with their respective RSDs within 5% limits. Finally, these validated methods were employed to test twenty six different commercial batches of DNV. The quality, reproducibility and consistency of DNV have been well established using these developed and reliable analytical tools. These analytical strategies successfully set a path forward for robust quality evaluation and standardization of Divya-Denguenil-Vati, and other related herbal formulations.</description><subject>Acids</subject><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromatography</subject><subject>Dengue fever</subject><subject>Formulations</subject><subject>Gallic acid</subject><subject>Laboratory Medicine</subject><subject>Mathematical analysis</subject><subject>Metabolites</subject><subject>Original</subject><subject>Pharmacy</subject><subject>Proteomics</subject><subject>Quality assessment</subject><subject>Rosmarinic acid</subject><subject>Standardization</subject><issn>0009-5893</issn><issn>1612-1112</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9Uk1vEzEUXCEqGgp_gJMlLhxq6o_dtZcDUklJUykVQU16tRz7bXC1sVt7N1L5UfxGTLYCwYGL_Z49Mx4_TVG8oeQ9JUScJUo4F5gwhklJJcfiWTGhNWWYUsqeFxNCSIMr2fDj4mVKd7llTV2_KI55JUUlOZ0UP64s-N61zujeBX-KbnXn7KFG2lt00-dVR-u-j2ehRZ9c0KZ3e0DXoQMzdJDQOjm_RevlYnp2fYO_rsLsFK3nuT2IzJerXDmPLtz-UeML8NsBvOvwbRb9gM7RMnvQeA5xozs0C3E3dONzbYhohKMZ7CG-Ko5a3SV4_bSfFOvZ59V0jhdfLq-m5wtsuKQ9thok5dYw0VDdmloC01raBipZVdw0bGNBt0wAtW0ela6M1boB0ghZSVNyflJ8HHXvh80OrMn-ou7UfXQ7HR9V0E79fePdN7UNe9XkCfNaZoF3TwIxPAyQerVzyUDXaQ9hSIrVTVnSmvA6Q9_-A70LQ_T5e4oJUouqFKLKKDaiTAwpRWh_m6FE_YqDGuOgchzUIQ5KZBIfSSmD_RbiH-n_sH4Czo-4FA</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Balkrishna, Acharya</creator><creator>Joshi, Monali</creator><creator>Tomer, Meenu</creator><creator>Verma, Sudeep</creator><creator>Gujral, Seema</creator><creator>Mulay, Vallabh Prakash</creator><creator>Srivastava, Jyotish</creator><creator>Varshney, Anurag</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2022</creationdate><title>Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever</title><author>Balkrishna, Acharya ; Joshi, Monali ; Tomer, Meenu ; Verma, Sudeep ; Gujral, Seema ; Mulay, Vallabh Prakash ; Srivastava, Jyotish ; Varshney, Anurag</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-dae813dc2791afc68e2aa8d9e58553c92bdeaf27e1df112a5cdaa9e097858c433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acids</topic><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromatography</topic><topic>Dengue fever</topic><topic>Formulations</topic><topic>Gallic acid</topic><topic>Laboratory Medicine</topic><topic>Mathematical analysis</topic><topic>Metabolites</topic><topic>Original</topic><topic>Pharmacy</topic><topic>Proteomics</topic><topic>Quality assessment</topic><topic>Rosmarinic acid</topic><topic>Standardization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balkrishna, Acharya</creatorcontrib><creatorcontrib>Joshi, Monali</creatorcontrib><creatorcontrib>Tomer, Meenu</creatorcontrib><creatorcontrib>Verma, Sudeep</creatorcontrib><creatorcontrib>Gujral, Seema</creatorcontrib><creatorcontrib>Mulay, Vallabh Prakash</creatorcontrib><creatorcontrib>Srivastava, Jyotish</creatorcontrib><creatorcontrib>Varshney, Anurag</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chromatographia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balkrishna, Acharya</au><au>Joshi, Monali</au><au>Tomer, Meenu</au><au>Verma, Sudeep</au><au>Gujral, Seema</au><au>Mulay, Vallabh Prakash</au><au>Srivastava, Jyotish</au><au>Varshney, Anurag</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever</atitle><jtitle>Chromatographia</jtitle><stitle>Chromatographia</stitle><date>2022</date><risdate>2022</risdate><volume>85</volume><issue>9</issue><spage>831</spage><epage>850</epage><pages>831-850</pages><issn>0009-5893</issn><eissn>1612-1112</eissn><abstract>For the last fifty years, Dengue has been one of the most common mosquito-borne arboviral infections which has spread over the tropical and subtropical world. Divya-Denguenil-Vati (DNV) has been formulated by blending five specific herbs for effective resolution of Dengue fever. In the present study, we aimed to identify, develop, validate, and standardize methods for Divya-Denguenil-Vati (DNV), on UHPLC and HPTLC analytical platforms, with rapid, sensitive, accurate and rugged attributes. At first, 97 phyto-constituents were identified by UPLC/MS-QToF in Divya-Denguenil-Vati. UHPLC method was then developed and validated for simultaneous determination of gallic acid, 5-HMF, protocatechuic acid, magnoflorine, methyl gallate, berberine, rutin, ellagic acid, β-ecdysone and rosmarinic acid in DNV. Four selected markers, gallic acid, rosmarinic acid, magnoflorine and rutin were further developed and validated on HPTLC. Analytical processes were validated as per ICH Q2 (R1) guidelines; and were found linear (
r
2
> 0.99), sensitive, precise (%RSD < 5%), and accurate, as indicated by high recovery values (88–105%). The limit of detection and quantification were also established for these phyto-metabolites, with their respective RSDs within 5% limits. Finally, these validated methods were employed to test twenty six different commercial batches of DNV. The quality, reproducibility and consistency of DNV have been well established using these developed and reliable analytical tools. These analytical strategies successfully set a path forward for robust quality evaluation and standardization of Divya-Denguenil-Vati, and other related herbal formulations.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35875831</pmid><doi>10.1007/s10337-022-04183-7</doi><tpages>20</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Analytical Chemistry Chemistry Chemistry and Materials Science Chromatography Dengue fever Formulations Gallic acid Laboratory Medicine Mathematical analysis Metabolites Original Pharmacy Proteomics Quality assessment Rosmarinic acid Standardization |
| title | Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever |
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