Synergetic integrations of bone marrow stem cells and transforming growth factor‐β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue
The cartilage repair and regeneration show inadequate self‐healing capability and have some complications, which are inordinate challenges in clinical therapy. Biopolymeric injectable hydrogels, a prominent type of cell‐carrier as well tissue engineering scaffolding materials, establish promising th...
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| Veröffentlicht in: | International wound journal 2022-08, Vol.19 (5), p.1023-1038 |
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| creator | Zheng, Dong Chen, Tong Han, Long Lv, Songwei Yin, Jianjian Yang, Kaiyuan Wang, Yuji Xu, Nanwei |
| description | The cartilage repair and regeneration show inadequate self‐healing capability and have some complications, which are inordinate challenges in clinical therapy. Biopolymeric injectable hydrogels, a prominent type of cell‐carrier as well tissue engineering scaffolding materials, establish promising therapeutic potential of stem cell‐based cartilage‐regeneration treatment. In addition, injectable scaffolding biomaterial should have rapid gelation properties with adequate rheological and mechanical properties. In the present investigation, we developed and fabricated the macromolecular silk fibroin blended with polylysine modified chitosan polymer (SF/PCS) using thermal‐sensitive glycerophosphate (GP), which contains effective gelation ability, morphology, porosity and also has enhanced mechanical properties to induce physical applicability, cell proliferation and nutrient exchange in the cell‐based treatment. The developed and optimised injectable hydrogel group has good biocompatibility with human fibroblast (L929) cells and bone marrow‐derived mesenchymal stem cells (BMSCs). Additionally, it was found that SF/PCS hydrogel group could sustainably release TGF‐β1 and efficiently regulate cartilage‐specific and inflammatory‐related gene expressions. Finally, the cartilage‐regeneration potential of the hydrogel groups embedded with and without BMSCs were evaluated in SD rat models under histopathological analysis, which showed promising cartilage repair. Overall, we conclude that the TGF‐β1‐SF/PCS injectable hydrogel demonstrates enhanced in vitro and in vivo tissue regeneration properties, which lead to efficacious therapeutic potential in cartilage regeneration. |
| doi_str_mv | 10.1111/iwj.13699 |
| format | Article |
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Biopolymeric injectable hydrogels, a prominent type of cell‐carrier as well tissue engineering scaffolding materials, establish promising therapeutic potential of stem cell‐based cartilage‐regeneration treatment. In addition, injectable scaffolding biomaterial should have rapid gelation properties with adequate rheological and mechanical properties. In the present investigation, we developed and fabricated the macromolecular silk fibroin blended with polylysine modified chitosan polymer (SF/PCS) using thermal‐sensitive glycerophosphate (GP), which contains effective gelation ability, morphology, porosity and also has enhanced mechanical properties to induce physical applicability, cell proliferation and nutrient exchange in the cell‐based treatment. The developed and optimised injectable hydrogel group has good biocompatibility with human fibroblast (L929) cells and bone marrow‐derived mesenchymal stem cells (BMSCs). Additionally, it was found that SF/PCS hydrogel group could sustainably release TGF‐β1 and efficiently regulate cartilage‐specific and inflammatory‐related gene expressions. Finally, the cartilage‐regeneration potential of the hydrogel groups embedded with and without BMSCs were evaluated in SD rat models under histopathological analysis, which showed promising cartilage repair. Overall, we conclude that the TGF‐β1‐SF/PCS injectable hydrogel demonstrates enhanced in vitro and in vivo tissue regeneration properties, which lead to efficacious therapeutic potential in cartilage regeneration.</description><identifier>ISSN: 1742-4801</identifier><identifier>EISSN: 1742-481X</identifier><identifier>DOI: 10.1111/iwj.13699</identifier><identifier>PMID: 35266304</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>articular cartilage ; Biological products ; Biomedical materials ; Biopolymers ; Bone marrow ; Cartilage ; Chemicals ; chitosan ; Growth factors ; Health aspects ; hydrogel ; Hydrogels ; Mechanical properties ; Morphology ; Nanoparticles ; Original ; Peptides ; Physiology ; Polymers ; Porosity ; Proteins ; Silk ; silk fibroin ; Stem cells ; Tissue engineering ; Transforming growth factors ; Transplantation</subject><ispartof>International wound journal, 2022-08, Vol.19 (5), p.1023-1038</ispartof><rights>2022 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd.</rights><rights>2021 The Authors. International Wound Journal published by Medicalhelplines.com Inc (3M) and John Wiley & Sons Ltd.</rights><rights>COPYRIGHT 2022 John Wiley & Sons, Inc.</rights><rights>2022. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5109-164aefd6d7eb018d23417364b206b33c4adbfb6cb66b51c26a2bc85394de1c473</citedby><cites>FETCH-LOGICAL-c5109-164aefd6d7eb018d23417364b206b33c4adbfb6cb66b51c26a2bc85394de1c473</cites><orcidid>0000-0003-3253-3357</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284642/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284642/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35266304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Dong</creatorcontrib><creatorcontrib>Chen, Tong</creatorcontrib><creatorcontrib>Han, Long</creatorcontrib><creatorcontrib>Lv, Songwei</creatorcontrib><creatorcontrib>Yin, Jianjian</creatorcontrib><creatorcontrib>Yang, Kaiyuan</creatorcontrib><creatorcontrib>Wang, Yuji</creatorcontrib><creatorcontrib>Xu, Nanwei</creatorcontrib><title>Synergetic integrations of bone marrow stem cells and transforming growth factor‐β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue</title><title>International wound journal</title><addtitle>Int Wound J</addtitle><description>The cartilage repair and regeneration show inadequate self‐healing capability and have some complications, which are inordinate challenges in clinical therapy. Biopolymeric injectable hydrogels, a prominent type of cell‐carrier as well tissue engineering scaffolding materials, establish promising therapeutic potential of stem cell‐based cartilage‐regeneration treatment. In addition, injectable scaffolding biomaterial should have rapid gelation properties with adequate rheological and mechanical properties. In the present investigation, we developed and fabricated the macromolecular silk fibroin blended with polylysine modified chitosan polymer (SF/PCS) using thermal‐sensitive glycerophosphate (GP), which contains effective gelation ability, morphology, porosity and also has enhanced mechanical properties to induce physical applicability, cell proliferation and nutrient exchange in the cell‐based treatment. The developed and optimised injectable hydrogel group has good biocompatibility with human fibroblast (L929) cells and bone marrow‐derived mesenchymal stem cells (BMSCs). Additionally, it was found that SF/PCS hydrogel group could sustainably release TGF‐β1 and efficiently regulate cartilage‐specific and inflammatory‐related gene expressions. Finally, the cartilage‐regeneration potential of the hydrogel groups embedded with and without BMSCs were evaluated in SD rat models under histopathological analysis, which showed promising cartilage repair. Overall, we conclude that the TGF‐β1‐SF/PCS injectable hydrogel demonstrates enhanced in vitro and in vivo tissue regeneration properties, which lead to efficacious therapeutic potential in cartilage regeneration.</description><subject>articular cartilage</subject><subject>Biological products</subject><subject>Biomedical materials</subject><subject>Biopolymers</subject><subject>Bone marrow</subject><subject>Cartilage</subject><subject>Chemicals</subject><subject>chitosan</subject><subject>Growth factors</subject><subject>Health aspects</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>Mechanical properties</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Original</subject><subject>Peptides</subject><subject>Physiology</subject><subject>Polymers</subject><subject>Porosity</subject><subject>Proteins</subject><subject>Silk</subject><subject>silk fibroin</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Transforming growth factors</subject><subject>Transplantation</subject><issn>1742-4801</issn><issn>1742-481X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNp1UkuOEzEQbSEQMwwsuAAqiRWLZOy2253eII1GfAaNxAIQ7CzbXd1xcOxgO4yy4wichYNwCFYcAycZAiOBvXDJ9eq9Z1dV1UNKprSsU3u1mFImuu5WdUxbXk_4jH64fYgJParupbQgpO6apr1bHbGmFoIRflz9fLPxGEfM1oD1Gceosg0-QRhAB4-wVDGGK0gZl2DQuQTK95Cj8mkIcWn9CGMB5DkMyuQQf3z5-v0bBRdUjz2Yuc0hKQ9e-bBSscg4TKAd-m06WfcRBqtjsL7IL9BkVXIw3_QxjOggB0A_V94gRFwpG3fqEUcsrndOYRUy-myVKwSwU1g7FcFsQ6dGhGxTWuP96s6gXMIH1-dJ9e75s7fnLyeXr19cnJ9dTkxDSTehgiscetG3qAmd9TXjtGWC65oIzZjhqteDFkYLoRtqaqFqbWYN63iP1PCWnVRP97yrtV5ib4q3qJxcRVt-ciODsvJmxtu5HMNn2dUzLnhdCB5fE8TwaY0py0VYR188S0Y6Iigprf6DGpVDaf0QCplZ2mTkWcspa7q2YwU1_Qeq7B6X1pT2Drbc3yh4si8wMaQUcTgYp0RuZ02WWZO7WSvYR3-_9ID8PVwFcLoHXBWVzf-Z5MX7V3vKX_Mj54c</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Zheng, Dong</creator><creator>Chen, Tong</creator><creator>Han, Long</creator><creator>Lv, Songwei</creator><creator>Yin, Jianjian</creator><creator>Yang, Kaiyuan</creator><creator>Wang, Yuji</creator><creator>Xu, Nanwei</creator><general>Blackwell Publishing Ltd</general><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3253-3357</orcidid></search><sort><creationdate>202208</creationdate><title>Synergetic integrations of bone marrow stem cells and transforming growth factor‐β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue</title><author>Zheng, Dong ; Chen, Tong ; Han, Long ; Lv, Songwei ; Yin, Jianjian ; Yang, Kaiyuan ; Wang, Yuji ; Xu, Nanwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5109-164aefd6d7eb018d23417364b206b33c4adbfb6cb66b51c26a2bc85394de1c473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>articular cartilage</topic><topic>Biological products</topic><topic>Biomedical materials</topic><topic>Biopolymers</topic><topic>Bone marrow</topic><topic>Cartilage</topic><topic>Chemicals</topic><topic>chitosan</topic><topic>Growth factors</topic><topic>Health aspects</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>Mechanical properties</topic><topic>Morphology</topic><topic>Nanoparticles</topic><topic>Original</topic><topic>Peptides</topic><topic>Physiology</topic><topic>Polymers</topic><topic>Porosity</topic><topic>Proteins</topic><topic>Silk</topic><topic>silk fibroin</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Transforming growth factors</topic><topic>Transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Dong</creatorcontrib><creatorcontrib>Chen, Tong</creatorcontrib><creatorcontrib>Han, Long</creatorcontrib><creatorcontrib>Lv, Songwei</creatorcontrib><creatorcontrib>Yin, Jianjian</creatorcontrib><creatorcontrib>Yang, Kaiyuan</creatorcontrib><creatorcontrib>Wang, Yuji</creatorcontrib><creatorcontrib>Xu, Nanwei</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International wound journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Dong</au><au>Chen, Tong</au><au>Han, Long</au><au>Lv, Songwei</au><au>Yin, Jianjian</au><au>Yang, Kaiyuan</au><au>Wang, Yuji</au><au>Xu, Nanwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synergetic integrations of bone marrow stem cells and transforming growth factor‐β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue</atitle><jtitle>International wound journal</jtitle><addtitle>Int Wound J</addtitle><date>2022-08</date><risdate>2022</risdate><volume>19</volume><issue>5</issue><spage>1023</spage><epage>1038</epage><pages>1023-1038</pages><issn>1742-4801</issn><eissn>1742-481X</eissn><abstract>The cartilage repair and regeneration show inadequate self‐healing capability and have some complications, which are inordinate challenges in clinical therapy. Biopolymeric injectable hydrogels, a prominent type of cell‐carrier as well tissue engineering scaffolding materials, establish promising therapeutic potential of stem cell‐based cartilage‐regeneration treatment. In addition, injectable scaffolding biomaterial should have rapid gelation properties with adequate rheological and mechanical properties. In the present investigation, we developed and fabricated the macromolecular silk fibroin blended with polylysine modified chitosan polymer (SF/PCS) using thermal‐sensitive glycerophosphate (GP), which contains effective gelation ability, morphology, porosity and also has enhanced mechanical properties to induce physical applicability, cell proliferation and nutrient exchange in the cell‐based treatment. The developed and optimised injectable hydrogel group has good biocompatibility with human fibroblast (L929) cells and bone marrow‐derived mesenchymal stem cells (BMSCs). Additionally, it was found that SF/PCS hydrogel group could sustainably release TGF‐β1 and efficiently regulate cartilage‐specific and inflammatory‐related gene expressions. Finally, the cartilage‐regeneration potential of the hydrogel groups embedded with and without BMSCs were evaluated in SD rat models under histopathological analysis, which showed promising cartilage repair. Overall, we conclude that the TGF‐β1‐SF/PCS injectable hydrogel demonstrates enhanced in vitro and in vivo tissue regeneration properties, which lead to efficacious therapeutic potential in cartilage regeneration.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>35266304</pmid><doi>10.1111/iwj.13699</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0003-3253-3357</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | articular cartilage Biological products Biomedical materials Biopolymers Bone marrow Cartilage Chemicals chitosan Growth factors Health aspects hydrogel Hydrogels Mechanical properties Morphology Nanoparticles Original Peptides Physiology Polymers Porosity Proteins Silk silk fibroin Stem cells Tissue engineering Transforming growth factors Transplantation |
| title | Synergetic integrations of bone marrow stem cells and transforming growth factor‐β1 loaded chitosan nanoparticles blended silk fibroin injectable hydrogel to enhance repair and regeneration potential in articular cartilage tissue |
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