Early and Polyantigenic CD4 T Cell Responses Correlate with Mild Disease in Acute COVID-19 Donors
We assessed SARS-CoV-2-specific CD4+ and CD8+ T cell responses in samples from 89 acute COVID-19 patients, utilizing blood samples collected during the first wave of COVID-19 in Italy. The goal of the study was to examine correlations between SARS-CoV-2-specific T cell responses in the early phase c...
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| Veröffentlicht in: | International journal of molecular sciences 2022-07, Vol.23 (13), p.7155 |
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| creator | Tarke, Alison Potesta, Marina Varchetta, Stefania Fenoglio, Daniela Iannetta, Marco Sarmati, Loredana Mele, Dalila Dentone, Chiara Bassetti, Matteo Montesano, Carla Mondelli, Mario U. Filaci, Gilberto Grifoni, Alba Sette, Alessandro |
| description | We assessed SARS-CoV-2-specific CD4+ and CD8+ T cell responses in samples from 89 acute COVID-19 patients, utilizing blood samples collected during the first wave of COVID-19 in Italy. The goal of the study was to examine correlations between SARS-CoV-2-specific T cell responses in the early phase comparing mild, moderate, or severe COVID-19 disease outcomes. T cell responses to the spike (S) and non-S proteins were measured in a combined activation-induced marker (AIM) and intracellular cytokine staining (ICS) assay. Early CD4+ T cell responses to SARS-CoV-2 S correlated with milder disease by both AIM and IFNγ ICS readouts. The correlation of S-specific CD4+ T cell responses with milder disease severity was most striking within the first two weeks of symptom onset compared to later time points. Furthermore, donors with milder disease were associated with polyantigenic CD4+ T cell responses that recognized more prominently non-S proteins in addition to S, while severe acute COVID-19 was characterized by lower magnitudes of CD4+ T cell responses and a narrower repertoire. In conclusion, this study highlights that both the magnitude and breadth of early SARS-CoV-2-specific CD4+ T cell responses correlated with milder disease outcomes in acute COVID-19 patients. |
| doi_str_mv | 10.3390/ijms23137155 |
| format | Article |
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The goal of the study was to examine correlations between SARS-CoV-2-specific T cell responses in the early phase comparing mild, moderate, or severe COVID-19 disease outcomes. T cell responses to the spike (S) and non-S proteins were measured in a combined activation-induced marker (AIM) and intracellular cytokine staining (ICS) assay. Early CD4+ T cell responses to SARS-CoV-2 S correlated with milder disease by both AIM and IFNγ ICS readouts. The correlation of S-specific CD4+ T cell responses with milder disease severity was most striking within the first two weeks of symptom onset compared to later time points. Furthermore, donors with milder disease were associated with polyantigenic CD4+ T cell responses that recognized more prominently non-S proteins in addition to S, while severe acute COVID-19 was characterized by lower magnitudes of CD4+ T cell responses and a narrower repertoire. In conclusion, this study highlights that both the magnitude and breadth of early SARS-CoV-2-specific CD4+ T cell responses correlated with milder disease outcomes in acute COVID-19 patients.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms23137155</identifier><identifier>PMID: 35806161</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Age ; CD4 antigen ; CD8 antigen ; Coronaviruses ; COVID-19 ; Cytokines ; Infections ; Lymphocytes ; Lymphocytes T ; Proteins ; Severe acute respiratory syndrome coronavirus 2 ; γ-Interferon</subject><ispartof>International journal of molecular sciences, 2022-07, Vol.23 (13), p.7155</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. 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The goal of the study was to examine correlations between SARS-CoV-2-specific T cell responses in the early phase comparing mild, moderate, or severe COVID-19 disease outcomes. T cell responses to the spike (S) and non-S proteins were measured in a combined activation-induced marker (AIM) and intracellular cytokine staining (ICS) assay. Early CD4+ T cell responses to SARS-CoV-2 S correlated with milder disease by both AIM and IFNγ ICS readouts. The correlation of S-specific CD4+ T cell responses with milder disease severity was most striking within the first two weeks of symptom onset compared to later time points. Furthermore, donors with milder disease were associated with polyantigenic CD4+ T cell responses that recognized more prominently non-S proteins in addition to S, while severe acute COVID-19 was characterized by lower magnitudes of CD4+ T cell responses and a narrower repertoire. In conclusion, this study highlights that both the magnitude and breadth of early SARS-CoV-2-specific CD4+ T cell responses correlated with milder disease outcomes in acute COVID-19 patients.</description><subject>Age</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Cytokines</subject><subject>Infections</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Proteins</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>γ-Interferon</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU9P3DAQxa0K1KWUGx_AEhcOpPWfxI4vSChL25VAILTlak2SCXiVtRc7Ae23b1YgBD3NSO-npzfzCDnm7IeUhv10q3USkkvNi-ILOeC5EBljSu992GfkW0orxoQUhflKZrIomeKKHxC4hNhvKfiW3oZ-C35wD-hdQ6t5Tpe0wr6nd5g2wSdMtAoxYg8D0hc3PNJr17d07hJCQuo8vWjGSapu7hfzjBs6Dz7E9J3sd9AnPHqbh-Tvr8tl9Se7uvm9qC6uskaWZsiA6ylT2zHN8gY6JrVgJi9rUTcGWWFKaIvOdK2sWVuihryuwRhVdygAEYU8JOevvpuxXmPboB8i9HYT3Rri1gZw9rPi3aN9CM_WCKWZlJPB6ZtBDE8jpsGuXWqmB4DHMCYrVKmnUKXSE3ryH7oKY_TTeTtKcSGlUhN19ko1MaQUsXsPw5nddWc_dif_ASD5isY</recordid><startdate>20220701</startdate><enddate>20220701</enddate><creator>Tarke, Alison</creator><creator>Potesta, Marina</creator><creator>Varchetta, Stefania</creator><creator>Fenoglio, Daniela</creator><creator>Iannetta, Marco</creator><creator>Sarmati, Loredana</creator><creator>Mele, Dalila</creator><creator>Dentone, Chiara</creator><creator>Bassetti, Matteo</creator><creator>Montesano, Carla</creator><creator>Mondelli, Mario U.</creator><creator>Filaci, Gilberto</creator><creator>Grifoni, Alba</creator><creator>Sette, Alessandro</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6445-8414</orcidid><orcidid>https://orcid.org/0000-0003-1452-0333</orcidid><orcidid>https://orcid.org/0000-0002-5217-2766</orcidid><orcidid>https://orcid.org/0000-0001-9532-6473</orcidid><orcidid>https://orcid.org/0000-0003-1811-3153</orcidid><orcidid>https://orcid.org/0000-0002-2209-5966</orcidid><orcidid>https://orcid.org/0000-0002-6938-8627</orcidid><orcidid>https://orcid.org/0000-0002-2464-6549</orcidid><orcidid>https://orcid.org/0000-0001-7013-2250</orcidid><orcidid>https://orcid.org/0000-0002-0289-2896</orcidid></search><sort><creationdate>20220701</creationdate><title>Early and Polyantigenic CD4 T Cell Responses Correlate with Mild Disease in Acute COVID-19 Donors</title><author>Tarke, Alison ; 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| subjects | Age CD4 antigen CD8 antigen Coronaviruses COVID-19 Cytokines Infections Lymphocytes Lymphocytes T Proteins Severe acute respiratory syndrome coronavirus 2 γ-Interferon |
| title | Early and Polyantigenic CD4 T Cell Responses Correlate with Mild Disease in Acute COVID-19 Donors |
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