Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction
Aflatoxin B1 (AFB 1 ) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of Pixian Douban , a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB 1 detection in Pixian Douban is influenced by the traditi...
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| Veröffentlicht in: | RSC advances 2022-06, Vol.12 (30), p.19528-19536 |
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| creator | Zeng, Chaoyi Xu, Chi Tian, Hongyun Shao, Kun Song, Yaning Yang, Xiao Che, Zhenming Huang, Yukun |
| description | Aflatoxin B1 (AFB
1
) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of
Pixian Douban
, a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB
1
detection in
Pixian Douban
is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB
1
in this sample, for which Fe
3
O
4
was synthesized
via
the solvothermal method and then a Fe
3
O
4
@SiO
2
–NH
2
with a core–shell structure was prepared, followed by an AFB
1
-aptamer attachment. The validation was performed
via
an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5–2.0 ng mL
−1
with
R
2
of 0.981, and recoveries of AFB
1
ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB
1
, facilitating quick and effective detection of AFB
1
in
Pixian DouBan
samples. |
| doi_str_mv | 10.1039/d2ra02763a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9258682</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2693775836</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-e6f92d7334d6a72d709c95d9bda4a0029a9ae6ce589162f56ef95f004982d4e93</originalsourceid><addsrcrecordid>eNpdkU1LxDAQhoMoKroXf0HAiwjVNGnT5iKsrl8gKKLnMNtMNdIma9LK-u-Nrog6h5mBeXh5Z4aQvZwd5UyoY8MDMF5JAWtkm7NCZpxJtf6r3yKTGF9YClnmXOabZEuUtSwlK7aJnuGAobcOBusd9S2FtoPBL62jpzlN-c4uLTg68-M8lTlENDSRsBigx0B7eHI42IZG31mTLZ4TQHE5BGg-FXfJRgtdxMl33SGPF-cPZ1fZze3l9dn0JmtELYYMZau4qYQojIQqdUw1qjRqbqAAxrgCBSgbLGuVS96WEltVtowVquamQCV2yMlKdzHOezQNuuSg04tgewjv2oPVfyfOPusn_6YVT7eoeRI4-BYI_nXEOOjexga7Dhz6MWoulaiqshYyofv_0Bc_BpfWS1RdF0LJIk_U4Ypqgo8xYPtjJmf683V6xu-nX6-big9AlIqQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2688439641</pqid></control><display><type>article</type><title>Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Zeng, Chaoyi ; Xu, Chi ; Tian, Hongyun ; Shao, Kun ; Song, Yaning ; Yang, Xiao ; Che, Zhenming ; Huang, Yukun</creator><creatorcontrib>Zeng, Chaoyi ; Xu, Chi ; Tian, Hongyun ; Shao, Kun ; Song, Yaning ; Yang, Xiao ; Che, Zhenming ; Huang, Yukun</creatorcontrib><description>Aflatoxin B1 (AFB
1
) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of
Pixian Douban
, a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB
1
detection in
Pixian Douban
is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB
1
in this sample, for which Fe
3
O
4
was synthesized
via
the solvothermal method and then a Fe
3
O
4
@SiO
2
–NH
2
with a core–shell structure was prepared, followed by an AFB
1
-aptamer attachment. The validation was performed
via
an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5–2.0 ng mL
−1
with
R
2
of 0.981, and recoveries of AFB
1
ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB
1
, facilitating quick and effective detection of AFB
1
in
Pixian DouBan
samples.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d2ra02763a</identifier><identifier>PMID: 35865604</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Aflatoxins ; Chemistry ; Core-shell structure ; Iron oxides ; Silicon dioxide ; Solid phases</subject><ispartof>RSC advances, 2022-06, Vol.12 (30), p.19528-19536</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><rights>This journal is © The Royal Society of Chemistry 2022 The Royal Society of Chemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-e6f92d7334d6a72d709c95d9bda4a0029a9ae6ce589162f56ef95f004982d4e93</citedby><cites>FETCH-LOGICAL-c383t-e6f92d7334d6a72d709c95d9bda4a0029a9ae6ce589162f56ef95f004982d4e93</cites><orcidid>0000-0001-7968-7260 ; 0000-0001-9723-5663</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258682/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258682/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Zeng, Chaoyi</creatorcontrib><creatorcontrib>Xu, Chi</creatorcontrib><creatorcontrib>Tian, Hongyun</creatorcontrib><creatorcontrib>Shao, Kun</creatorcontrib><creatorcontrib>Song, Yaning</creatorcontrib><creatorcontrib>Yang, Xiao</creatorcontrib><creatorcontrib>Che, Zhenming</creatorcontrib><creatorcontrib>Huang, Yukun</creatorcontrib><title>Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction</title><title>RSC advances</title><description>Aflatoxin B1 (AFB
1
) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of
Pixian Douban
, a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB
1
detection in
Pixian Douban
is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB
1
in this sample, for which Fe
3
O
4
was synthesized
via
the solvothermal method and then a Fe
3
O
4
@SiO
2
–NH
2
with a core–shell structure was prepared, followed by an AFB
1
-aptamer attachment. The validation was performed
via
an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5–2.0 ng mL
−1
with
R
2
of 0.981, and recoveries of AFB
1
ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB
1
, facilitating quick and effective detection of AFB
1
in
Pixian DouBan
samples.</description><subject>Aflatoxins</subject><subject>Chemistry</subject><subject>Core-shell structure</subject><subject>Iron oxides</subject><subject>Silicon dioxide</subject><subject>Solid phases</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkU1LxDAQhoMoKroXf0HAiwjVNGnT5iKsrl8gKKLnMNtMNdIma9LK-u-Nrog6h5mBeXh5Z4aQvZwd5UyoY8MDMF5JAWtkm7NCZpxJtf6r3yKTGF9YClnmXOabZEuUtSwlK7aJnuGAobcOBusd9S2FtoPBL62jpzlN-c4uLTg68-M8lTlENDSRsBigx0B7eHI42IZG31mTLZ4TQHE5BGg-FXfJRgtdxMl33SGPF-cPZ1fZze3l9dn0JmtELYYMZau4qYQojIQqdUw1qjRqbqAAxrgCBSgbLGuVS96WEltVtowVquamQCV2yMlKdzHOezQNuuSg04tgewjv2oPVfyfOPusn_6YVT7eoeRI4-BYI_nXEOOjexga7Dhz6MWoulaiqshYyofv_0Bc_BpfWS1RdF0LJIk_U4Ypqgo8xYPtjJmf683V6xu-nX6-big9AlIqQ</recordid><startdate>20220629</startdate><enddate>20220629</enddate><creator>Zeng, Chaoyi</creator><creator>Xu, Chi</creator><creator>Tian, Hongyun</creator><creator>Shao, Kun</creator><creator>Song, Yaning</creator><creator>Yang, Xiao</creator><creator>Che, Zhenming</creator><creator>Huang, Yukun</creator><general>Royal Society of Chemistry</general><general>The Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7968-7260</orcidid><orcidid>https://orcid.org/0000-0001-9723-5663</orcidid></search><sort><creationdate>20220629</creationdate><title>Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction</title><author>Zeng, Chaoyi ; Xu, Chi ; Tian, Hongyun ; Shao, Kun ; Song, Yaning ; Yang, Xiao ; Che, Zhenming ; Huang, Yukun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-e6f92d7334d6a72d709c95d9bda4a0029a9ae6ce589162f56ef95f004982d4e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aflatoxins</topic><topic>Chemistry</topic><topic>Core-shell structure</topic><topic>Iron oxides</topic><topic>Silicon dioxide</topic><topic>Solid phases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Chaoyi</creatorcontrib><creatorcontrib>Xu, Chi</creatorcontrib><creatorcontrib>Tian, Hongyun</creatorcontrib><creatorcontrib>Shao, Kun</creatorcontrib><creatorcontrib>Song, Yaning</creatorcontrib><creatorcontrib>Yang, Xiao</creatorcontrib><creatorcontrib>Che, Zhenming</creatorcontrib><creatorcontrib>Huang, Yukun</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Chaoyi</au><au>Xu, Chi</au><au>Tian, Hongyun</au><au>Shao, Kun</au><au>Song, Yaning</au><au>Yang, Xiao</au><au>Che, Zhenming</au><au>Huang, Yukun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction</atitle><jtitle>RSC advances</jtitle><date>2022-06-29</date><risdate>2022</risdate><volume>12</volume><issue>30</issue><spage>19528</spage><epage>19536</epage><pages>19528-19536</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Aflatoxin B1 (AFB
1
) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of
Pixian Douban
, a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB
1
detection in
Pixian Douban
is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB
1
in this sample, for which Fe
3
O
4
was synthesized
via
the solvothermal method and then a Fe
3
O
4
@SiO
2
–NH
2
with a core–shell structure was prepared, followed by an AFB
1
-aptamer attachment. The validation was performed
via
an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5–2.0 ng mL
−1
with
R
2
of 0.981, and recoveries of AFB
1
ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB
1
, facilitating quick and effective detection of AFB
1
in
Pixian DouBan
samples.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>35865604</pmid><doi>10.1039/d2ra02763a</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7968-7260</orcidid><orcidid>https://orcid.org/0000-0001-9723-5663</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Aflatoxins Chemistry Core-shell structure Iron oxides Silicon dioxide Solid phases |
| title | Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction |
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