Synchrotron X-ray biosample imaging: opportunities and challenges
Synchrotron radiation phase-contrast microtomography is sensitive to low attenuating tissues, giving an alternative visualisation of the sample and being useful for investigating microstructure inside biological specimens without staining them with a contrast medium. The phase-contrast technique has...
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| Veröffentlicht in: | Biophysical reviews 2022-06, Vol.14 (3), p.625-633 |
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| creator | Sena, Gabriela Fidalgo, Gabriel Paiva, Katrine Barcelos, Renan Nogueira, Liebert Parreiras Colaço, Marcos Vinícius Gonzalez, Marcelo Salabert Azambuja, Patricia Colaço, Gustavo da Silva, Helio Ricardo de Moura Meneses, Anderson Alvarenga Barroso, Regina Cély |
| description | Synchrotron radiation phase-contrast microtomography is sensitive to low attenuating tissues, giving an alternative visualisation of the sample and being useful for investigating microstructure inside biological specimens without staining them with a contrast medium. The phase-contrast technique has been widely used in the scientific community, as it is a technique associated with radiography and microscopy and able to enhance contrast in soft tissues, specifically at the edges, showing details that could not be seen by the absorption technique. This work aims to show the ability of synchrotron-based phase-contrast microtomography for the visualisation of soft tissues and hard internal structures of millimetre-sized biological organisms. Case studies of the anatomy of
Rhodnius prolixus
head and
Thoropa miliaris
tadpole are presented to illustrate the imaging technique. |
| doi_str_mv | 10.1007/s12551-022-00964-4 |
| format | Article |
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Rhodnius prolixus
head and
Thoropa miliaris
tadpole are presented to illustrate the imaging technique.</description><identifier>ISSN: 1867-2450</identifier><identifier>EISSN: 1867-2469</identifier><identifier>DOI: 10.1007/s12551-022-00964-4</identifier><identifier>PMID: 35791381</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biochemistry ; Biological and Medical Physics ; Biological Techniques ; Biomedical and Life Sciences ; Biophysics ; Cell Biology ; Contrast media ; Imaging techniques ; Letter to the Editor ; Life Sciences ; Membrane Biology ; Microtomography ; Nanotechnology ; Radiography ; Soft tissues ; Synchrotron radiation ; Synchrotrons ; Visualization ; X ray imagery</subject><ispartof>Biophysical reviews, 2022-06, Vol.14 (3), p.625-633</ispartof><rights>International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2022</rights><rights>International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany, part of Springer Nature 2022.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-fb5dc11ae866263b5ccc9d4c22e87d66cb9974ef2856209449cb934a11852d373</citedby><cites>FETCH-LOGICAL-c474t-fb5dc11ae866263b5ccc9d4c22e87d66cb9974ef2856209449cb934a11852d373</cites><orcidid>0000-0001-6348-694X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250583/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250583/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35791381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sena, Gabriela</creatorcontrib><creatorcontrib>Fidalgo, Gabriel</creatorcontrib><creatorcontrib>Paiva, Katrine</creatorcontrib><creatorcontrib>Barcelos, Renan</creatorcontrib><creatorcontrib>Nogueira, Liebert Parreiras</creatorcontrib><creatorcontrib>Colaço, Marcos Vinícius</creatorcontrib><creatorcontrib>Gonzalez, Marcelo Salabert</creatorcontrib><creatorcontrib>Azambuja, Patricia</creatorcontrib><creatorcontrib>Colaço, Gustavo</creatorcontrib><creatorcontrib>da Silva, Helio Ricardo</creatorcontrib><creatorcontrib>de Moura Meneses, Anderson Alvarenga</creatorcontrib><creatorcontrib>Barroso, Regina Cély</creatorcontrib><title>Synchrotron X-ray biosample imaging: opportunities and challenges</title><title>Biophysical reviews</title><addtitle>Biophys Rev</addtitle><addtitle>Biophys Rev</addtitle><description>Synchrotron radiation phase-contrast microtomography is sensitive to low attenuating tissues, giving an alternative visualisation of the sample and being useful for investigating microstructure inside biological specimens without staining them with a contrast medium. The phase-contrast technique has been widely used in the scientific community, as it is a technique associated with radiography and microscopy and able to enhance contrast in soft tissues, specifically at the edges, showing details that could not be seen by the absorption technique. This work aims to show the ability of synchrotron-based phase-contrast microtomography for the visualisation of soft tissues and hard internal structures of millimetre-sized biological organisms. Case studies of the anatomy of
Rhodnius prolixus
head and
Thoropa miliaris
tadpole are presented to illustrate the imaging technique.</description><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Cell Biology</subject><subject>Contrast media</subject><subject>Imaging techniques</subject><subject>Letter to the Editor</subject><subject>Life Sciences</subject><subject>Membrane Biology</subject><subject>Microtomography</subject><subject>Nanotechnology</subject><subject>Radiography</subject><subject>Soft tissues</subject><subject>Synchrotron radiation</subject><subject>Synchrotrons</subject><subject>Visualization</subject><subject>X ray imagery</subject><issn>1867-2450</issn><issn>1867-2469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kUtLAzEUhYMotlb_gAsZcONmNO9JXAgivkBwoYK7kMmk7ZRpMiYzQv-9qa31sXCVkPvdc8_NAeAQwVMEYXEWEWYM5RDjHELJaU63wBAJXuSYcrm9uTM4AHsxziDkFAu2CwaEFRIRgYbg8mnhzDT4LniXveZBL7Ky9lHP28Zm9VxPajc5z3zb-tD1ru5qGzPtqsxMddNYN7FxH-yMdRPtwfocgZeb6-eru_zh8fb-6vIhN7SgXT4uWWUQ0lZwjjkpmTFGVtRgbEVRcW5KKQtqx8kgx1BSKtMLoRohwXBFCjICFyvdti_ntjLWdUE3qg3JZVgor2v1u-LqqZr4dyUxg0yQJHCyFgj-rbexU_M6Gts02lnfR4W5YJRywmRCj_-gM98Hl9ZbUoSLgguYKLyiTPAxBjvemEFQLRNSq4RUSkh9JqRoajr6ucam5SuSBJAVEFMpfXD4nv2P7Acr_pxG</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Sena, Gabriela</creator><creator>Fidalgo, Gabriel</creator><creator>Paiva, Katrine</creator><creator>Barcelos, Renan</creator><creator>Nogueira, Liebert Parreiras</creator><creator>Colaço, Marcos Vinícius</creator><creator>Gonzalez, Marcelo Salabert</creator><creator>Azambuja, Patricia</creator><creator>Colaço, Gustavo</creator><creator>da Silva, Helio Ricardo</creator><creator>de Moura Meneses, Anderson Alvarenga</creator><creator>Barroso, Regina Cély</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6348-694X</orcidid></search><sort><creationdate>20220601</creationdate><title>Synchrotron X-ray biosample imaging: opportunities and challenges</title><author>Sena, Gabriela ; 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Rhodnius prolixus
head and
Thoropa miliaris
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| subjects | Biochemistry Biological and Medical Physics Biological Techniques Biomedical and Life Sciences Biophysics Cell Biology Contrast media Imaging techniques Letter to the Editor Life Sciences Membrane Biology Microtomography Nanotechnology Radiography Soft tissues Synchrotron radiation Synchrotrons Visualization X ray imagery |
| title | Synchrotron X-ray biosample imaging: opportunities and challenges |
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