Diacetyl and α-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in α-acetolactate decarboxylase and have a low lactate dehydrogenase activity
Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate...
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| Veröffentlicht in: | Applied and environmental microbiology 2000-12, Vol.66 (12), p.5518-5520 |
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| creator | MONNET, Christophe AYMES, Frédéric CORRIEU, Georges |
| description | Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha-acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin. |
| doi_str_mv | 10.1128/AEM.66.12.5518-5520.2000 |
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Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha-acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.66.12.5518-5520.2000</identifier><identifier>PMID: 11097941</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>a-Acetolactate ; a-acetolactate dehydrogenase ; a-Acetolactic acid ; Aerobiosis ; Anaerobiosis ; Biological and medical sciences ; Carboxy-Lyases - genetics ; Carboxy-Lyases - metabolism ; Culture Media ; diacetyl ; Diacetyl - metabolism ; Ecology, environment ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; Kinetics ; L-Lactate Dehydrogenase - metabolism ; Lactates - metabolism ; Lactococcus lactis ; Lactococcus lactis - genetics ; Lactococcus lactis - metabolism ; Life Sciences ; nitrosoguanidine ; random mutagenesis</subject><ispartof>Applied and environmental microbiology, 2000-12, Vol.66 (12), p.5518-5520</ispartof><rights>2001 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2000, American Society for Microbiology 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-83a8ad72e7a27d766e4abad8148f755264fef524f52a8d28590f62b64c41af803</citedby><cites>FETCH-LOGICAL-c507t-83a8ad72e7a27d766e4abad8148f755264fef524f52a8d28590f62b64c41af803</cites><orcidid>0000-0003-2647-272X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC92495/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC92495/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=851224$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11097941$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02691205$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>MONNET, Christophe</creatorcontrib><creatorcontrib>AYMES, Frédéric</creatorcontrib><creatorcontrib>CORRIEU, Georges</creatorcontrib><title>Diacetyl and α-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in α-acetolactate decarboxylase and have a low lactate dehydrogenase activity</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha-acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin.</description><subject>a-Acetolactate</subject><subject>a-acetolactate dehydrogenase</subject><subject>a-Acetolactic acid</subject><subject>Aerobiosis</subject><subject>Anaerobiosis</subject><subject>Biological and medical sciences</subject><subject>Carboxy-Lyases - genetics</subject><subject>Carboxy-Lyases - metabolism</subject><subject>Culture Media</subject><subject>diacetyl</subject><subject>Diacetyl - metabolism</subject><subject>Ecology, environment</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Lactates - metabolism</subject><subject>Lactococcus lactis</subject><subject>Lactococcus lactis - genetics</subject><subject>Lactococcus lactis - metabolism</subject><subject>Life Sciences</subject><subject>nitrosoguanidine</subject><subject>random mutagenesis</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkt-O1CAUxonRuOPqKxgSExMvWoG2lCbeTNbVNRnjjV6TU6A7mE4ZgVb7WL7IPpN0p-7654LAOfy-czjkQwhTklPKxOvt5cec85yyvKqoyKqKkZwRQh6gDSVNShQFf4g2hDRNxlhJztCTEL4moCRcPEZnNFF1U9INunlrQZk49xgGjW9-ZkvkelARosFuMv7onR5VtG7A7Yx36cYpp9QY8ELZgMPYhmP-O2qtm8BjvZY9JQ9jhCEGHPcQMXiDtemssmaI2A7_ddVGgW_dj6QO5vZde5jSAffuO76H9rP27toMt1BqM9k4P0WPOuiDebbu5-jLu8vPF1fZ7tP7DxfbXaYqUsdMFCBA18zUwGpdc25KaEELWoquTr_Jy850FSvTAqGZqBrScdbyUpUUOkGKc_TmVPc4tgejVZrEQy-P3h7Az9KBlX_fDHYvr90kG1Y2VZK_Osn3_4iutju55AjjDWWkmmhiX66tvPs2mhDlwQZl-h4G48YgaS0KTgqWQHEClXcheNPdVaZELraRyTaSc0mZXGwjF9vIxTZJ-vzPce6Fq08S8GIFICjoOw-DsuGOExVNNit-ATYp0zw</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>MONNET, Christophe</creator><creator>AYMES, Frédéric</creator><creator>CORRIEU, Georges</creator><general>American Society for Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2647-272X</orcidid></search><sort><creationdate>20001201</creationdate><title>Diacetyl and α-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in α-acetolactate decarboxylase and have a low lactate dehydrogenase activity</title><author>MONNET, Christophe ; AYMES, Frédéric ; CORRIEU, Georges</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-83a8ad72e7a27d766e4abad8148f755264fef524f52a8d28590f62b64c41af803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>a-Acetolactate</topic><topic>a-acetolactate dehydrogenase</topic><topic>a-Acetolactic acid</topic><topic>Aerobiosis</topic><topic>Anaerobiosis</topic><topic>Biological and medical sciences</topic><topic>Carboxy-Lyases - genetics</topic><topic>Carboxy-Lyases - metabolism</topic><topic>Culture Media</topic><topic>diacetyl</topic><topic>Diacetyl - metabolism</topic><topic>Ecology, environment</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kinetics</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Lactates - metabolism</topic><topic>Lactococcus lactis</topic><topic>Lactococcus lactis - genetics</topic><topic>Lactococcus lactis - metabolism</topic><topic>Life Sciences</topic><topic>nitrosoguanidine</topic><topic>random mutagenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MONNET, Christophe</creatorcontrib><creatorcontrib>AYMES, Frédéric</creatorcontrib><creatorcontrib>CORRIEU, Georges</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MONNET, Christophe</au><au>AYMES, Frédéric</au><au>CORRIEU, Georges</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diacetyl and α-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in α-acetolactate decarboxylase and have a low lactate dehydrogenase activity</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>66</volume><issue>12</issue><spage>5518</spage><epage>5520</epage><pages>5518-5520</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha-acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>11097941</pmid><doi>10.1128/AEM.66.12.5518-5520.2000</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0003-2647-272X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | a-Acetolactate a-acetolactate dehydrogenase a-Acetolactic acid Aerobiosis Anaerobiosis Biological and medical sciences Carboxy-Lyases - genetics Carboxy-Lyases - metabolism Culture Media diacetyl Diacetyl - metabolism Ecology, environment Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Kinetics L-Lactate Dehydrogenase - metabolism Lactates - metabolism Lactococcus lactis Lactococcus lactis - genetics Lactococcus lactis - metabolism Life Sciences nitrosoguanidine random mutagenesis |
| title | Diacetyl and α-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in α-acetolactate decarboxylase and have a low lactate dehydrogenase activity |
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