Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope
Objectives: To evaluate the sensitivity of the real-time PCR assay melting curve method in the diagnosis of pulmonary tuberculosis (PTB) by analyzing bronchoalveolar lavage fluid (BALF) obtained from bronchoscopy. Methods: A total of 214 PTB patients who were treated at Xuancheng People’s Hospital r...
Gespeichert in:
| Veröffentlicht in: | Pakistan journal of medical sciences 2022-05, Vol.38 (5), p.1333-1337 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1337 |
|---|---|
| container_issue | 5 |
| container_start_page | 1333 |
| container_title | Pakistan journal of medical sciences |
| container_volume | 38 |
| creator | Wei, Jun Sun, Jun Shuai, Xuefen Ren, Junqing Chen, Xuedong |
| description | Objectives: To evaluate the sensitivity of the real-time PCR assay melting curve method in the diagnosis of pulmonary tuberculosis (PTB) by analyzing bronchoalveolar lavage fluid (BALF) obtained from bronchoscopy.
Methods: A total of 214 PTB patients who were treated at Xuancheng People’s Hospital respiratory and infection department during January 2018 to January 2021 were included in this study. Bronchoscopic bronchoalveolar lavage fluid (BALF) examined by polymerase chain reaction (melting curve method), BALF smear, BALF culture, lipoarabinomannan (LAM) antigen test for diagnosis of tuberculosis (TB) (LAM-TB), acid-fast stain (AFS), and serum adenosine deaminase (ADA) test were conducted respectively to compare their positive predictive values (PPVs).
Results: Of the 214 patients with confirmed PTB, 84.11% were BALF melting curve method positive, significantly higher than the positive results yielded by other PTB screening tests, i.e., LAM-TB (69.16%), AFS (51.87%), ADA (49.07%), BALF culture (62.15%), and BALF smear (41.12%) (p |
| doi_str_mv | 10.12669/pjms.38.5.5480 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9247759</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A709056883</galeid><sourcerecordid>A709056883</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-4107afc3e65a12c433a5d50ac618e48f551faa617370b408a4b0df74b7d501a33</originalsourceid><addsrcrecordid>eNpdkk-L2zAQxU1p6S7bPfcqKJTswVnJsiz5UkhC_0GgS5vC3sRYlm0tspRKtiEfpN-3drIU2tMMvN-8GYmXJG8JXpOsKMr741Mf11Ss2ZrlAr9IrkkhSMoJe3x57rMUZ_njVXIbo6kwxoxizsnr5IoyXpackevk9w_tohnMZIYT8g162H1H4MCeoolo1Ws7GNciNYZJo14Pna_vkHGoNtA6vzDLzGh77yCc0GGsdFCjPSurh8P2DlUQdY28Q9vgneo82El7CwHtYYJWo9V2s5-pE6ouelT-qN8krxqwUd8-15vk56ePh92XdP_t89fdZp8qKsSQ5gRzaBTVBQOSqZxSYDXDoAoidC4axkgDUBBOOa5yLCCvcN3wvOIzRYDSm-TDxfc4Vr2ulXZDACuPwfTzc6QHI_9VnOlk6ydZZjnnrJwNVs8Gwf8adRxkb6LS1oLTfowyKwTnGc-KZde7_9AnP4b5q89UJnBGSD5T7y9UC1bLToMduujtOBjvotxwXGJWCLHY3V9AFXyMQTd_ryZYnuMhl3hIKiSTSzzoH3utrdo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2682802114</pqid></control><display><type>article</type><title>Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope</title><source>PMC (PubMed Central)</source><source>DOAJ Directory of Open Access Journals</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Wei, Jun ; Sun, Jun ; Shuai, Xuefen ; Ren, Junqing ; Chen, Xuedong</creator><creatorcontrib>Wei, Jun ; Sun, Jun ; Shuai, Xuefen ; Ren, Junqing ; Chen, Xuedong</creatorcontrib><description>Objectives: To evaluate the sensitivity of the real-time PCR assay melting curve method in the diagnosis of pulmonary tuberculosis (PTB) by analyzing bronchoalveolar lavage fluid (BALF) obtained from bronchoscopy.
Methods: A total of 214 PTB patients who were treated at Xuancheng People’s Hospital respiratory and infection department during January 2018 to January 2021 were included in this study. Bronchoscopic bronchoalveolar lavage fluid (BALF) examined by polymerase chain reaction (melting curve method), BALF smear, BALF culture, lipoarabinomannan (LAM) antigen test for diagnosis of tuberculosis (TB) (LAM-TB), acid-fast stain (AFS), and serum adenosine deaminase (ADA) test were conducted respectively to compare their positive predictive values (PPVs).
Results: Of the 214 patients with confirmed PTB, 84.11% were BALF melting curve method positive, significantly higher than the positive results yielded by other PTB screening tests, i.e., LAM-TB (69.16%), AFS (51.87%), ADA (49.07%), BALF culture (62.15%), and BALF smear (41.12%) (p<0.05, respectively). The PPVs were increased to 92.06%, 93.93%, 92.99%, 95.79%, and 91.12% when BALF melting curve method was performed in combination with LAM-TB, AFS, ADA, BALF culture, and BALF smear, respectively, significantly higher than that produced by BALF melting curve method or the combined use of any two of the non-BALF melting curve method tests (p<0.05, respectively).
Conclusion: BALF melting curve method is an ideal diagnostic approach to PTB, which is of a higher diagnostic value compared with LAM-TB, AFS, ADA, BALF culture, and BALF smear.
doi: https://doi.org/10.12669/pjms.38.5.5480
How to cite this:Wei J, Sun J, Shuai X, Ren J, Chen X. Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope. Pak J Med Sci. 2022;38(5):---. doi: https://doi.org/10.12669/pjms.38.5.5480
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</description><identifier>ISSN: 1682-024X</identifier><identifier>EISSN: 1681-715X</identifier><identifier>DOI: 10.12669/pjms.38.5.5480</identifier><identifier>PMID: 35799751</identifier><language>eng</language><publisher>Karachi: Knowledge Bylanes</publisher><subject>Abscesses ; Accuracy ; Bacterial infections ; Care and treatment ; Diagnosis ; Diagnostic equipment (Medical) ; Endocarditis ; Fistula ; Medical diagnosis ; Medical imaging ; Meta-analysis ; Methods ; Original ; Prostheses ; Pulmonary tuberculosis ; Radiation ; Surgery ; Tomography ; Tuberculosis ; Vegetation</subject><ispartof>Pakistan journal of medical sciences, 2022-05, Vol.38 (5), p.1333-1337</ispartof><rights>COPYRIGHT 2022 Knowledge Bylanes</rights><rights>(c)2022 Pakistan Journal of Medical Sciences</rights><rights>Copyright: © Pakistan Journal of Medical Sciences 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-4107afc3e65a12c433a5d50ac618e48f551faa617370b408a4b0df74b7d501a33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247759/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247759/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Wei, Jun</creatorcontrib><creatorcontrib>Sun, Jun</creatorcontrib><creatorcontrib>Shuai, Xuefen</creatorcontrib><creatorcontrib>Ren, Junqing</creatorcontrib><creatorcontrib>Chen, Xuedong</creatorcontrib><title>Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope</title><title>Pakistan journal of medical sciences</title><description>Objectives: To evaluate the sensitivity of the real-time PCR assay melting curve method in the diagnosis of pulmonary tuberculosis (PTB) by analyzing bronchoalveolar lavage fluid (BALF) obtained from bronchoscopy.
Methods: A total of 214 PTB patients who were treated at Xuancheng People’s Hospital respiratory and infection department during January 2018 to January 2021 were included in this study. Bronchoscopic bronchoalveolar lavage fluid (BALF) examined by polymerase chain reaction (melting curve method), BALF smear, BALF culture, lipoarabinomannan (LAM) antigen test for diagnosis of tuberculosis (TB) (LAM-TB), acid-fast stain (AFS), and serum adenosine deaminase (ADA) test were conducted respectively to compare their positive predictive values (PPVs).
Results: Of the 214 patients with confirmed PTB, 84.11% were BALF melting curve method positive, significantly higher than the positive results yielded by other PTB screening tests, i.e., LAM-TB (69.16%), AFS (51.87%), ADA (49.07%), BALF culture (62.15%), and BALF smear (41.12%) (p<0.05, respectively). The PPVs were increased to 92.06%, 93.93%, 92.99%, 95.79%, and 91.12% when BALF melting curve method was performed in combination with LAM-TB, AFS, ADA, BALF culture, and BALF smear, respectively, significantly higher than that produced by BALF melting curve method or the combined use of any two of the non-BALF melting curve method tests (p<0.05, respectively).
Conclusion: BALF melting curve method is an ideal diagnostic approach to PTB, which is of a higher diagnostic value compared with LAM-TB, AFS, ADA, BALF culture, and BALF smear.
doi: https://doi.org/10.12669/pjms.38.5.5480
How to cite this:Wei J, Sun J, Shuai X, Ren J, Chen X. Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope. Pak J Med Sci. 2022;38(5):---. doi: https://doi.org/10.12669/pjms.38.5.5480
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</description><subject>Abscesses</subject><subject>Accuracy</subject><subject>Bacterial infections</subject><subject>Care and treatment</subject><subject>Diagnosis</subject><subject>Diagnostic equipment (Medical)</subject><subject>Endocarditis</subject><subject>Fistula</subject><subject>Medical diagnosis</subject><subject>Medical imaging</subject><subject>Meta-analysis</subject><subject>Methods</subject><subject>Original</subject><subject>Prostheses</subject><subject>Pulmonary tuberculosis</subject><subject>Radiation</subject><subject>Surgery</subject><subject>Tomography</subject><subject>Tuberculosis</subject><subject>Vegetation</subject><issn>1682-024X</issn><issn>1681-715X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkk-L2zAQxU1p6S7bPfcqKJTswVnJsiz5UkhC_0GgS5vC3sRYlm0tspRKtiEfpN-3drIU2tMMvN-8GYmXJG8JXpOsKMr741Mf11Ss2ZrlAr9IrkkhSMoJe3x57rMUZ_njVXIbo6kwxoxizsnr5IoyXpackevk9w_tohnMZIYT8g162H1H4MCeoolo1Ws7GNciNYZJo14Pna_vkHGoNtA6vzDLzGh77yCc0GGsdFCjPSurh8P2DlUQdY28Q9vgneo82El7CwHtYYJWo9V2s5-pE6ouelT-qN8krxqwUd8-15vk56ePh92XdP_t89fdZp8qKsSQ5gRzaBTVBQOSqZxSYDXDoAoidC4axkgDUBBOOa5yLCCvcN3wvOIzRYDSm-TDxfc4Vr2ulXZDACuPwfTzc6QHI_9VnOlk6ydZZjnnrJwNVs8Gwf8adRxkb6LS1oLTfowyKwTnGc-KZde7_9AnP4b5q89UJnBGSD5T7y9UC1bLToMduujtOBjvotxwXGJWCLHY3V9AFXyMQTd_ryZYnuMhl3hIKiSTSzzoH3utrdo</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Wei, Jun</creator><creator>Sun, Jun</creator><creator>Shuai, Xuefen</creator><creator>Ren, Junqing</creator><creator>Chen, Xuedong</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><general>Professional Medical Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220501</creationdate><title>Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope</title><author>Wei, Jun ; Sun, Jun ; Shuai, Xuefen ; Ren, Junqing ; Chen, Xuedong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-4107afc3e65a12c433a5d50ac618e48f551faa617370b408a4b0df74b7d501a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abscesses</topic><topic>Accuracy</topic><topic>Bacterial infections</topic><topic>Care and treatment</topic><topic>Diagnosis</topic><topic>Diagnostic equipment (Medical)</topic><topic>Endocarditis</topic><topic>Fistula</topic><topic>Medical diagnosis</topic><topic>Medical imaging</topic><topic>Meta-analysis</topic><topic>Methods</topic><topic>Original</topic><topic>Prostheses</topic><topic>Pulmonary tuberculosis</topic><topic>Radiation</topic><topic>Surgery</topic><topic>Tomography</topic><topic>Tuberculosis</topic><topic>Vegetation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Jun</creatorcontrib><creatorcontrib>Sun, Jun</creatorcontrib><creatorcontrib>Shuai, Xuefen</creatorcontrib><creatorcontrib>Ren, Junqing</creatorcontrib><creatorcontrib>Chen, Xuedong</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pakistan journal of medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Jun</au><au>Sun, Jun</au><au>Shuai, Xuefen</au><au>Ren, Junqing</au><au>Chen, Xuedong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope</atitle><jtitle>Pakistan journal of medical sciences</jtitle><date>2022-05-01</date><risdate>2022</risdate><volume>38</volume><issue>5</issue><spage>1333</spage><epage>1337</epage><pages>1333-1337</pages><issn>1682-024X</issn><eissn>1681-715X</eissn><abstract>Objectives: To evaluate the sensitivity of the real-time PCR assay melting curve method in the diagnosis of pulmonary tuberculosis (PTB) by analyzing bronchoalveolar lavage fluid (BALF) obtained from bronchoscopy.
Methods: A total of 214 PTB patients who were treated at Xuancheng People’s Hospital respiratory and infection department during January 2018 to January 2021 were included in this study. Bronchoscopic bronchoalveolar lavage fluid (BALF) examined by polymerase chain reaction (melting curve method), BALF smear, BALF culture, lipoarabinomannan (LAM) antigen test for diagnosis of tuberculosis (TB) (LAM-TB), acid-fast stain (AFS), and serum adenosine deaminase (ADA) test were conducted respectively to compare their positive predictive values (PPVs).
Results: Of the 214 patients with confirmed PTB, 84.11% were BALF melting curve method positive, significantly higher than the positive results yielded by other PTB screening tests, i.e., LAM-TB (69.16%), AFS (51.87%), ADA (49.07%), BALF culture (62.15%), and BALF smear (41.12%) (p<0.05, respectively). The PPVs were increased to 92.06%, 93.93%, 92.99%, 95.79%, and 91.12% when BALF melting curve method was performed in combination with LAM-TB, AFS, ADA, BALF culture, and BALF smear, respectively, significantly higher than that produced by BALF melting curve method or the combined use of any two of the non-BALF melting curve method tests (p<0.05, respectively).
Conclusion: BALF melting curve method is an ideal diagnostic approach to PTB, which is of a higher diagnostic value compared with LAM-TB, AFS, ADA, BALF culture, and BALF smear.
doi: https://doi.org/10.12669/pjms.38.5.5480
How to cite this:Wei J, Sun J, Shuai X, Ren J, Chen X. Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope. Pak J Med Sci. 2022;38(5):---. doi: https://doi.org/10.12669/pjms.38.5.5480
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</abstract><cop>Karachi</cop><pub>Knowledge Bylanes</pub><pmid>35799751</pmid><doi>10.12669/pjms.38.5.5480</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1682-024X |
| ispartof | Pakistan journal of medical sciences, 2022-05, Vol.38 (5), p.1333-1337 |
| issn | 1682-024X 1681-715X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9247759 |
| source | PMC (PubMed Central); DOAJ Directory of Open Access Journals; Alma/SFX Local Collection; PubMed Central Open Access |
| subjects | Abscesses Accuracy Bacterial infections Care and treatment Diagnosis Diagnostic equipment (Medical) Endocarditis Fistula Medical diagnosis Medical imaging Meta-analysis Methods Original Prostheses Pulmonary tuberculosis Radiation Surgery Tomography Tuberculosis Vegetation |
| title | Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T06%3A29%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitivity%20of%20PCR%20analysis%20(melting%20curve%20method)%20in%20diagnosis%20of%20Pulmonary%20Tuberculosis%20(PTB)%20based%20on%20Bronchoalveolar%20Lavage%20(BAL)%20by%20bronchoscope&rft.jtitle=Pakistan%20journal%20of%20medical%20sciences&rft.au=Wei,%20Jun&rft.date=2022-05-01&rft.volume=38&rft.issue=5&rft.spage=1333&rft.epage=1337&rft.pages=1333-1337&rft.issn=1682-024X&rft.eissn=1681-715X&rft_id=info:doi/10.12669/pjms.38.5.5480&rft_dat=%3Cgale_pubme%3EA709056883%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2682802114&rft_id=info:pmid/35799751&rft_galeid=A709056883&rfr_iscdi=true |