CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity

CRISPR-Cas12a (Cpf1) is a bacterial RNA-guided nuclease that cuts double-stranded DNA (dsDNA) at sites specified by a CRISPR RNA (crRNA) guide. Additional activities have been ascribed to this enzyme in vitro: site-specific (cis) single-stranded DNA (ssDNA) cleavage and indiscriminate (trans) degrad...

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Veröffentlicht in:	Nucleic acids research 2022-06, Vol.50 (11), p.6414-6422
Hauptverfasser:	Marino, Nicole D, Pinilla-Redondo, Rafael, Bondy-Denomy, Joseph
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - metabolism CRISPR-Associated Proteins - genetics CRISPR-Associated Proteins - metabolism CRISPR-Cas Systems DNA DNA, Single-Stranded - genetics Escherichia coli - genetics Escherichia coli - metabolism Immunity - genetics Nucleic Acid Enzymes Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - metabolism RNA, Guide, CRISPR-Cas Systems - genetics
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