Development and immunogenicity evaluation of porcine deltacoronavirus inactivated vaccine with different adjuvants in mice
Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in pigs of various ages, especially in suckling piglets, and there are no effective measures to prevent and control PDCoV currently. In this study, two adjuvants Al(OH)3 and ODN2395 working through different mechanisms were...
Gespeichert in:
| Veröffentlicht in: | Vaccine 2022-07, Vol.40 (31), p.4211-4219 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4219 |
|---|---|
| container_issue | 31 |
| container_start_page | 4211 |
| container_title | Vaccine |
| container_volume | 40 |
| creator | Zhao, Fu-jie Liu, Lin-tao Wang, Zi Wang, Nian-xiang Ma, Meng-yao Jia, Xin-hao Lu, Si-jia Xiang, Yu-qiang Zheng, Lan-lan Hu, Hui |
| description | Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in pigs of various ages, especially in suckling piglets, and there are no effective measures to prevent and control PDCoV currently. In this study, two adjuvants Al(OH)3 and ODN2395 working through different mechanisms were used to prepare inactivated PDCoV vaccines, and the immune effects of PDCoV inactivated vaccines were assessed in mice. From the results, we found that both PDCoV/Al(OH)3 vaccine and PDCoV/2395 vaccine could induce IgG and neutralizing antibodies with high levels in mice. At the same time, cytokines of IFN-γ, IL-4 and chemokine ligand of CXCL13 in serum were significantly increased after immunization, and reached the highest levels in PDCoV/2395 vaccine group, which suggested that PDCoV/2395 could promote the production of both Th1 and Th2 polarized cytokines. In addition, histopathological observations showed that vaccination helped mice resist PDCoV infection. These results indicated that both the two inactivated vaccines have good immune effects. Moreover, the PDCoV/2395 vaccine worked better than the PDCoV/Al(OH)3 vaccine for PDCoV/2395 having the good ability to induce both humoral and cellular immunogenicity. The PDCoV/2395 inactivated vaccine developed in this study might be an effective tool for the prevention of PDCoV infection. |
| doi_str_mv | 10.1016/j.vaccine.2022.05.085 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9181634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0264410X22007216</els_id><sourcerecordid>2676931031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c495t-73662da4cd570217e1898ee876ee7bf333bee3e67762de53c8c74850373a086d3</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhS0EokPhJ4AssWGTYMfxIxtQVZ5SJTYgsbM89k3rKLEHxwkqvx6HGSpgw8oLf_fcc89B6CklNSVUvBzq1VjrA9QNaZqa8Joofg_tqJKsajhV99GONKKtWkq-nqFH8zwQQjij3UN0xrjoNnCHfryBFcZ4mCBkbILDfpqWEK8heOvzLYbVjIvJPgYce3yIaVuJHYzZ2JhiMKtPy4x9MDb71WRw-OQLf_f5Bjvf95B-ibthWU3IG4wnb-ExetCbcYYnp_ccfXn39vPlh-rq0_uPlxdXlW07nivJhGicaa3jkjRUAlWdAlBSAMh9zxjbAzAQUhYMOLPKylZxwiQzRAnHztGro-5h2U_gbDGTzKgPyU8m3epovP77J_gbfR1XXSKigrVF4MVJIMVvC8xZT362MI4mQFxm3QjJO9W2lBb0-T_oEJcUynkbJTpGCdsofqRsivOcoL8zQ4ne2tWDPqWot3Y14bq0W-ae_XnJ3dTvOgvw-ghAyXP1kPRsPQQLziewWbvo_7PiJ65GvGw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2676931031</pqid></control><display><type>article</type><title>Development and immunogenicity evaluation of porcine deltacoronavirus inactivated vaccine with different adjuvants in mice</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>ProQuest Central UK/Ireland</source><creator>Zhao, Fu-jie ; Liu, Lin-tao ; Wang, Zi ; Wang, Nian-xiang ; Ma, Meng-yao ; Jia, Xin-hao ; Lu, Si-jia ; Xiang, Yu-qiang ; Zheng, Lan-lan ; Hu, Hui</creator><creatorcontrib>Zhao, Fu-jie ; Liu, Lin-tao ; Wang, Zi ; Wang, Nian-xiang ; Ma, Meng-yao ; Jia, Xin-hao ; Lu, Si-jia ; Xiang, Yu-qiang ; Zheng, Lan-lan ; Hu, Hui</creatorcontrib><description>Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in pigs of various ages, especially in suckling piglets, and there are no effective measures to prevent and control PDCoV currently. In this study, two adjuvants Al(OH)3 and ODN2395 working through different mechanisms were used to prepare inactivated PDCoV vaccines, and the immune effects of PDCoV inactivated vaccines were assessed in mice. From the results, we found that both PDCoV/Al(OH)3 vaccine and PDCoV/2395 vaccine could induce IgG and neutralizing antibodies with high levels in mice. At the same time, cytokines of IFN-γ, IL-4 and chemokine ligand of CXCL13 in serum were significantly increased after immunization, and reached the highest levels in PDCoV/2395 vaccine group, which suggested that PDCoV/2395 could promote the production of both Th1 and Th2 polarized cytokines. In addition, histopathological observations showed that vaccination helped mice resist PDCoV infection. These results indicated that both the two inactivated vaccines have good immune effects. Moreover, the PDCoV/2395 vaccine worked better than the PDCoV/Al(OH)3 vaccine for PDCoV/2395 having the good ability to induce both humoral and cellular immunogenicity. The PDCoV/2395 inactivated vaccine developed in this study might be an effective tool for the prevention of PDCoV infection.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2022.05.085</identifier><identifier>PMID: 35691873</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adjuvants ; Al(OH)3 ; Animal vaccines ; Animals ; Antibodies ; Antigens ; Chemokines ; Coronaviruses ; COVID-19 ; CXCL13 protein ; Cytokines ; Cytotoxicity ; Deactivation ; Deltacoronavirus ; Diarrhea ; Hogs ; Immunization ; Immunogenicity ; Immunoglobulin G ; Inactivated vaccine ; Interleukin 4 ; Laboratory animals ; Ligands ; Lymphocytes ; Lymphocytes T ; Mice ; ODN2395 ; PDCoV ; Suckling behavior ; Swine ; Swine Diseases ; Vaccines ; Vaccines, Inactivated ; Viruses ; γ-Interferon</subject><ispartof>Vaccine, 2022-07, Vol.40 (31), p.4211-4219</ispartof><rights>2022 Elsevier Ltd</rights><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><rights>2022. Elsevier Ltd</rights><rights>2022 Elsevier Ltd. All rights reserved. 2022 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-73662da4cd570217e1898ee876ee7bf333bee3e67762de53c8c74850373a086d3</citedby><cites>FETCH-LOGICAL-c495t-73662da4cd570217e1898ee876ee7bf333bee3e67762de53c8c74850373a086d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2676931031?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35691873$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Fu-jie</creatorcontrib><creatorcontrib>Liu, Lin-tao</creatorcontrib><creatorcontrib>Wang, Zi</creatorcontrib><creatorcontrib>Wang, Nian-xiang</creatorcontrib><creatorcontrib>Ma, Meng-yao</creatorcontrib><creatorcontrib>Jia, Xin-hao</creatorcontrib><creatorcontrib>Lu, Si-jia</creatorcontrib><creatorcontrib>Xiang, Yu-qiang</creatorcontrib><creatorcontrib>Zheng, Lan-lan</creatorcontrib><creatorcontrib>Hu, Hui</creatorcontrib><title>Development and immunogenicity evaluation of porcine deltacoronavirus inactivated vaccine with different adjuvants in mice</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in pigs of various ages, especially in suckling piglets, and there are no effective measures to prevent and control PDCoV currently. In this study, two adjuvants Al(OH)3 and ODN2395 working through different mechanisms were used to prepare inactivated PDCoV vaccines, and the immune effects of PDCoV inactivated vaccines were assessed in mice. From the results, we found that both PDCoV/Al(OH)3 vaccine and PDCoV/2395 vaccine could induce IgG and neutralizing antibodies with high levels in mice. At the same time, cytokines of IFN-γ, IL-4 and chemokine ligand of CXCL13 in serum were significantly increased after immunization, and reached the highest levels in PDCoV/2395 vaccine group, which suggested that PDCoV/2395 could promote the production of both Th1 and Th2 polarized cytokines. In addition, histopathological observations showed that vaccination helped mice resist PDCoV infection. These results indicated that both the two inactivated vaccines have good immune effects. Moreover, the PDCoV/2395 vaccine worked better than the PDCoV/Al(OH)3 vaccine for PDCoV/2395 having the good ability to induce both humoral and cellular immunogenicity. The PDCoV/2395 inactivated vaccine developed in this study might be an effective tool for the prevention of PDCoV infection.</description><subject>Adjuvants</subject><subject>Al(OH)3</subject><subject>Animal vaccines</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Chemokines</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>CXCL13 protein</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Deactivation</subject><subject>Deltacoronavirus</subject><subject>Diarrhea</subject><subject>Hogs</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunoglobulin G</subject><subject>Inactivated vaccine</subject><subject>Interleukin 4</subject><subject>Laboratory animals</subject><subject>Ligands</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Mice</subject><subject>ODN2395</subject><subject>PDCoV</subject><subject>Suckling behavior</subject><subject>Swine</subject><subject>Swine Diseases</subject><subject>Vaccines</subject><subject>Vaccines, Inactivated</subject><subject>Viruses</subject><subject>γ-Interferon</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtv1DAUhS0EokPhJ4AssWGTYMfxIxtQVZ5SJTYgsbM89k3rKLEHxwkqvx6HGSpgw8oLf_fcc89B6CklNSVUvBzq1VjrA9QNaZqa8Joofg_tqJKsajhV99GONKKtWkq-nqFH8zwQQjij3UN0xrjoNnCHfryBFcZ4mCBkbILDfpqWEK8heOvzLYbVjIvJPgYce3yIaVuJHYzZ2JhiMKtPy4x9MDb71WRw-OQLf_f5Bjvf95B-ibthWU3IG4wnb-ExetCbcYYnp_ccfXn39vPlh-rq0_uPlxdXlW07nivJhGicaa3jkjRUAlWdAlBSAMh9zxjbAzAQUhYMOLPKylZxwiQzRAnHztGro-5h2U_gbDGTzKgPyU8m3epovP77J_gbfR1XXSKigrVF4MVJIMVvC8xZT362MI4mQFxm3QjJO9W2lBb0-T_oEJcUynkbJTpGCdsofqRsivOcoL8zQ4ne2tWDPqWot3Y14bq0W-ae_XnJ3dTvOgvw-ghAyXP1kPRsPQQLziewWbvo_7PiJ65GvGw</recordid><startdate>20220729</startdate><enddate>20220729</enddate><creator>Zhao, Fu-jie</creator><creator>Liu, Lin-tao</creator><creator>Wang, Zi</creator><creator>Wang, Nian-xiang</creator><creator>Ma, Meng-yao</creator><creator>Jia, Xin-hao</creator><creator>Lu, Si-jia</creator><creator>Xiang, Yu-qiang</creator><creator>Zheng, Lan-lan</creator><creator>Hu, Hui</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20220729</creationdate><title>Development and immunogenicity evaluation of porcine deltacoronavirus inactivated vaccine with different adjuvants in mice</title><author>Zhao, Fu-jie ; Liu, Lin-tao ; Wang, Zi ; Wang, Nian-xiang ; Ma, Meng-yao ; Jia, Xin-hao ; Lu, Si-jia ; Xiang, Yu-qiang ; Zheng, Lan-lan ; Hu, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-73662da4cd570217e1898ee876ee7bf333bee3e67762de53c8c74850373a086d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adjuvants</topic><topic>Al(OH)3</topic><topic>Animal vaccines</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Chemokines</topic><topic>Coronaviruses</topic><topic>COVID-19</topic><topic>CXCL13 protein</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Deactivation</topic><topic>Deltacoronavirus</topic><topic>Diarrhea</topic><topic>Hogs</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunoglobulin G</topic><topic>Inactivated vaccine</topic><topic>Interleukin 4</topic><topic>Laboratory animals</topic><topic>Ligands</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Mice</topic><topic>ODN2395</topic><topic>PDCoV</topic><topic>Suckling behavior</topic><topic>Swine</topic><topic>Swine Diseases</topic><topic>Vaccines</topic><topic>Vaccines, Inactivated</topic><topic>Viruses</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Fu-jie</creatorcontrib><creatorcontrib>Liu, Lin-tao</creatorcontrib><creatorcontrib>Wang, Zi</creatorcontrib><creatorcontrib>Wang, Nian-xiang</creatorcontrib><creatorcontrib>Ma, Meng-yao</creatorcontrib><creatorcontrib>Jia, Xin-hao</creatorcontrib><creatorcontrib>Lu, Si-jia</creatorcontrib><creatorcontrib>Xiang, Yu-qiang</creatorcontrib><creatorcontrib>Zheng, Lan-lan</creatorcontrib><creatorcontrib>Hu, Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Fu-jie</au><au>Liu, Lin-tao</au><au>Wang, Zi</au><au>Wang, Nian-xiang</au><au>Ma, Meng-yao</au><au>Jia, Xin-hao</au><au>Lu, Si-jia</au><au>Xiang, Yu-qiang</au><au>Zheng, Lan-lan</au><au>Hu, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and immunogenicity evaluation of porcine deltacoronavirus inactivated vaccine with different adjuvants in mice</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2022-07-29</date><risdate>2022</risdate><volume>40</volume><issue>31</issue><spage>4211</spage><epage>4219</epage><pages>4211-4219</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in pigs of various ages, especially in suckling piglets, and there are no effective measures to prevent and control PDCoV currently. In this study, two adjuvants Al(OH)3 and ODN2395 working through different mechanisms were used to prepare inactivated PDCoV vaccines, and the immune effects of PDCoV inactivated vaccines were assessed in mice. From the results, we found that both PDCoV/Al(OH)3 vaccine and PDCoV/2395 vaccine could induce IgG and neutralizing antibodies with high levels in mice. At the same time, cytokines of IFN-γ, IL-4 and chemokine ligand of CXCL13 in serum were significantly increased after immunization, and reached the highest levels in PDCoV/2395 vaccine group, which suggested that PDCoV/2395 could promote the production of both Th1 and Th2 polarized cytokines. In addition, histopathological observations showed that vaccination helped mice resist PDCoV infection. These results indicated that both the two inactivated vaccines have good immune effects. Moreover, the PDCoV/2395 vaccine worked better than the PDCoV/Al(OH)3 vaccine for PDCoV/2395 having the good ability to induce both humoral and cellular immunogenicity. The PDCoV/2395 inactivated vaccine developed in this study might be an effective tool for the prevention of PDCoV infection.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>35691873</pmid><doi>10.1016/j.vaccine.2022.05.085</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2022-07, Vol.40 (31), p.4211-4219 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9181634 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; ProQuest Central UK/Ireland |
| subjects | Adjuvants Al(OH)3 Animal vaccines Animals Antibodies Antigens Chemokines Coronaviruses COVID-19 CXCL13 protein Cytokines Cytotoxicity Deactivation Deltacoronavirus Diarrhea Hogs Immunization Immunogenicity Immunoglobulin G Inactivated vaccine Interleukin 4 Laboratory animals Ligands Lymphocytes Lymphocytes T Mice ODN2395 PDCoV Suckling behavior Swine Swine Diseases Vaccines Vaccines, Inactivated Viruses γ-Interferon |
| title | Development and immunogenicity evaluation of porcine deltacoronavirus inactivated vaccine with different adjuvants in mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T08%3A07%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20immunogenicity%20evaluation%20of%20porcine%20deltacoronavirus%20inactivated%20vaccine%20with%20different%20adjuvants%20in%20mice&rft.jtitle=Vaccine&rft.au=Zhao,%20Fu-jie&rft.date=2022-07-29&rft.volume=40&rft.issue=31&rft.spage=4211&rft.epage=4219&rft.pages=4211-4219&rft.issn=0264-410X&rft.eissn=1873-2518&rft_id=info:doi/10.1016/j.vaccine.2022.05.085&rft_dat=%3Cproquest_pubme%3E2676931031%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2676931031&rft_id=info:pmid/35691873&rft_els_id=S0264410X22007216&rfr_iscdi=true |