Technical note: A comparison between rehydrating solutions in the pretreatment of mummified and corified skin for forensic microscopic examination

Microscopic examination of mummified or corified skin may be of extreme importance for forensic purposes. However, standard histological samples in these cases are low-end, and preparation is burdened by several problems and so are diagnostic results: an improvement of these types of specimens is th...

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Veröffentlicht in:International journal of legal medicine 2022-07, Vol.136 (4), p.997-1007
Hauptverfasser: Tambuzzi, Stefano, Gentile, Guendalina, Bilardo, Gianluigi, Boracchi, Michele, Bailo, Paolo, Casalino, Teresa, Andreola, Salvatore, Zoja, Riccardo
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container_issue 4
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container_title International journal of legal medicine
container_volume 136
creator Tambuzzi, Stefano
Gentile, Guendalina
Bilardo, Gianluigi
Boracchi, Michele
Bailo, Paolo
Casalino, Teresa
Andreola, Salvatore
Zoja, Riccardo
description Microscopic examination of mummified or corified skin may be of extreme importance for forensic purposes. However, standard histological samples in these cases are low-end, and preparation is burdened by several problems and so are diagnostic results: an improvement of these types of specimens is therefore advantageous. This study aims to identify the best performing rehydration solution among a fabric softener, a body lotion, and Sandison’s rehydrating solution. Samples of skin undergoing mummification or corification were collected from 25 corpses and each sample was divided into 4 fragments: one of these fragments was directly fixated in 4% formalin, one was previously treated with a tissue softener, another one was previously treated with a body lotion, and the last one was treated with Sandison’s solution. After 72 h, the pretreated samples were post-fixated in 4% formalin and then prepared for standard histological examination staining the histological slides with hematoxylin-eosin and Masson’s trichrome. At the microscopic examination, samples directly fixated in formalin were characterized by usual marked structural alterations and altered stainability, typical of such dry tissues. Vice versa, those previously treated appeared to be better-preserved even though with different improvement levels: body lotion made a medium–low-grade restoration of the tissues, and fabric softener a high-grade restoration, while Sandison’s rehydrating solution produced an optimal grade restoration. Sandison’s rehydrating solution was confirmed to be the best rehydrating substance for mummified and corified skin. Fabric softener could be, however, considered a valid substitute, being productive of high-grade microscopic yield.
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source HeinOnline Law Journal Library; SpringerNature Journals
subjects Forensic Medicine
Forensic pathology
Fragments
Hydration
Medical Law
Medicine
Medicine & Public Health
Method Paper
Microscopy
Restoration
Skin
Softening agents
title Technical note: A comparison between rehydrating solutions in the pretreatment of mummified and corified skin for forensic microscopic examination
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