Repair of surviving hair cells in the damaged mouse utricle
Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and...
Gespeichert in:
Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2022-04, Vol.119 (15), p.1-12 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 12 |
---|---|
container_issue | 15 |
container_start_page | 1 |
container_title | Proceedings of the National Academy of Sciences - PNAS |
container_volume | 119 |
creator | Kim, Grace S. Wang, Tian Sayyid, Zahra N. Fuhriman, Jessica Jones, Sherri M. Cheng, Alan G. |
description | Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and contribute to this recovery is unclear. Here, we generated the Pou4f3DTR/+; Atoh1CreERTM/+; Rosa26RtdTomato/+
mouse to fate map HCs prior to ablation. After HC ablation, vestibular evoked potentials were abolished in all animals, with ∼57% later recovering responses. Relative to nonrecovery mice, recovery animals harbored more Atoh1-tdTomato⁺ surviving HCs. In both groups, surviving HCs displayed markers of both type I and type II subtypes and afferent synapses, despite distorted lamination and morphology. Surviving type II HCs remained innervated in both groups, whereas surviving type I HCs first lacked and later regained calyces in the recovery, but not the nonrecovery, group. Finally, surviving HCs initially displayed immature and subsequently mature-appearing bundles in the recovery group. These results demonstrate that surviving HCs are capable of self-repair and may contribute to the recovery of vestibular function. |
doi_str_mv | 10.1073/pnas.2116973119 |
format | Article |
fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9169652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>27151826</jstor_id><sourcerecordid>27151826</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-6166a26625d2bcbf04817e4152a331fcaf1cd37837538788b105965fe5f144b73</originalsourceid><addsrcrecordid>eNpdkUtLxDAUhYMoOo6uXSkFN26quXkHQRDxBYIgug5pm85k6GNM2gH_vS2j42N14d4vh3NyEDoCfA5Y0otlY-M5ARBaUgC9hSaANaSCabyNJhgTmSpG2B7aj3GBMdZc4V20RzlVWGk5QZcvbml9SNoyiX1Y-ZVvZsl83OSuqmLim6Sbu6SwtZ25IqnbPrqk74LPK3eAdkpbRXf4Nafo7e729eYhfXq-f7y5fkpzxmiXChDCEiEIL0iWZyVmCqRjwImlFMrclpAXVCoqB1dSqQww14KXjpfAWCbpFF2tdZd9Vrsid00XbGWWwdc2fJjWevP30vi5mbUro4d_EZwMAmdfAqF9713sTO3jmM82bghkiGBScC71iJ7-QxdtH5oh3kBxSimXcnR0saby0MYYXLkxA9iMxZixGPNTzPDi5HeGDf_dxAAcr4FF7NqwuRMJHBQR9BNWMpIc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2653335777</pqid></control><display><type>article</type><title>Repair of surviving hair cells in the damaged mouse utricle</title><source>MEDLINE</source><source>PMC (PubMed Central)</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Kim, Grace S. ; Wang, Tian ; Sayyid, Zahra N. ; Fuhriman, Jessica ; Jones, Sherri M. ; Cheng, Alan G.</creator><creatorcontrib>Kim, Grace S. ; Wang, Tian ; Sayyid, Zahra N. ; Fuhriman, Jessica ; Jones, Sherri M. ; Cheng, Alan G.</creatorcontrib><description>Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and contribute to this recovery is unclear. Here, we generated the Pou4f3DTR/+; Atoh1CreERTM/+; Rosa26RtdTomato/+
mouse to fate map HCs prior to ablation. After HC ablation, vestibular evoked potentials were abolished in all animals, with ∼57% later recovering responses. Relative to nonrecovery mice, recovery animals harbored more Atoh1-tdTomato⁺ surviving HCs. In both groups, surviving HCs displayed markers of both type I and type II subtypes and afferent synapses, despite distorted lamination and morphology. Surviving type II HCs remained innervated in both groups, whereas surviving type I HCs first lacked and later regained calyces in the recovery, but not the nonrecovery, group. Finally, surviving HCs initially displayed immature and subsequently mature-appearing bundles in the recovery group. These results demonstrate that surviving HCs are capable of self-repair and may contribute to the recovery of vestibular function.</description><identifier>ISSN: 0027-8424</identifier><identifier>ISSN: 1091-6490</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.2116973119</identifier><identifier>PMID: 35380897</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Ablation ; Animals ; Basic Helix-Loop-Helix Transcription Factors - genetics ; Biological Sciences ; Cell Survival - genetics ; Damage detection ; Hair ; Hair cells ; Hair Cells, Vestibular - physiology ; Homeodomain Proteins - genetics ; Lamination ; Math1 protein ; Mechanoreceptors ; Mice ; Mice, Mutant Strains ; Recovery ; Regeneration - genetics ; Repair ; RNA, Untranslated - genetics ; Saccule and Utricle - cytology ; Saccule and Utricle - injuries ; Saccule and Utricle - physiology ; Sensory neurons ; Survival ; Synapses ; Transcription Factor Brn-3C - genetics ; Utricle ; Vestibular system</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2022-04, Vol.119 (15), p.1-12</ispartof><rights>Copyright © 2022 the Author(s)</rights><rights>Copyright National Academy of Sciences Apr 12, 2022</rights><rights>Copyright © 2022 the Author(s). Published by PNAS. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-6166a26625d2bcbf04817e4152a331fcaf1cd37837538788b105965fe5f144b73</citedby><cites>FETCH-LOGICAL-c443t-6166a26625d2bcbf04817e4152a331fcaf1cd37837538788b105965fe5f144b73</cites><orcidid>0000-0001-8784-2953 ; 0000-0002-4702-8401 ; 0000-0001-6872-7028</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169652/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169652/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35380897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Grace S.</creatorcontrib><creatorcontrib>Wang, Tian</creatorcontrib><creatorcontrib>Sayyid, Zahra N.</creatorcontrib><creatorcontrib>Fuhriman, Jessica</creatorcontrib><creatorcontrib>Jones, Sherri M.</creatorcontrib><creatorcontrib>Cheng, Alan G.</creatorcontrib><title>Repair of surviving hair cells in the damaged mouse utricle</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and contribute to this recovery is unclear. Here, we generated the Pou4f3DTR/+; Atoh1CreERTM/+; Rosa26RtdTomato/+
mouse to fate map HCs prior to ablation. After HC ablation, vestibular evoked potentials were abolished in all animals, with ∼57% later recovering responses. Relative to nonrecovery mice, recovery animals harbored more Atoh1-tdTomato⁺ surviving HCs. In both groups, surviving HCs displayed markers of both type I and type II subtypes and afferent synapses, despite distorted lamination and morphology. Surviving type II HCs remained innervated in both groups, whereas surviving type I HCs first lacked and later regained calyces in the recovery, but not the nonrecovery, group. Finally, surviving HCs initially displayed immature and subsequently mature-appearing bundles in the recovery group. These results demonstrate that surviving HCs are capable of self-repair and may contribute to the recovery of vestibular function.</description><subject>Ablation</subject><subject>Animals</subject><subject>Basic Helix-Loop-Helix Transcription Factors - genetics</subject><subject>Biological Sciences</subject><subject>Cell Survival - genetics</subject><subject>Damage detection</subject><subject>Hair</subject><subject>Hair cells</subject><subject>Hair Cells, Vestibular - physiology</subject><subject>Homeodomain Proteins - genetics</subject><subject>Lamination</subject><subject>Math1 protein</subject><subject>Mechanoreceptors</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Recovery</subject><subject>Regeneration - genetics</subject><subject>Repair</subject><subject>RNA, Untranslated - genetics</subject><subject>Saccule and Utricle - cytology</subject><subject>Saccule and Utricle - injuries</subject><subject>Saccule and Utricle - physiology</subject><subject>Sensory neurons</subject><subject>Survival</subject><subject>Synapses</subject><subject>Transcription Factor Brn-3C - genetics</subject><subject>Utricle</subject><subject>Vestibular system</subject><issn>0027-8424</issn><issn>1091-6490</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtLxDAUhYMoOo6uXSkFN26quXkHQRDxBYIgug5pm85k6GNM2gH_vS2j42N14d4vh3NyEDoCfA5Y0otlY-M5ARBaUgC9hSaANaSCabyNJhgTmSpG2B7aj3GBMdZc4V20RzlVWGk5QZcvbml9SNoyiX1Y-ZVvZsl83OSuqmLim6Sbu6SwtZ25IqnbPrqk74LPK3eAdkpbRXf4Nafo7e729eYhfXq-f7y5fkpzxmiXChDCEiEIL0iWZyVmCqRjwImlFMrclpAXVCoqB1dSqQww14KXjpfAWCbpFF2tdZd9Vrsid00XbGWWwdc2fJjWevP30vi5mbUro4d_EZwMAmdfAqF9713sTO3jmM82bghkiGBScC71iJ7-QxdtH5oh3kBxSimXcnR0saby0MYYXLkxA9iMxZixGPNTzPDi5HeGDf_dxAAcr4FF7NqwuRMJHBQR9BNWMpIc</recordid><startdate>20220412</startdate><enddate>20220412</enddate><creator>Kim, Grace S.</creator><creator>Wang, Tian</creator><creator>Sayyid, Zahra N.</creator><creator>Fuhriman, Jessica</creator><creator>Jones, Sherri M.</creator><creator>Cheng, Alan G.</creator><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8784-2953</orcidid><orcidid>https://orcid.org/0000-0002-4702-8401</orcidid><orcidid>https://orcid.org/0000-0001-6872-7028</orcidid></search><sort><creationdate>20220412</creationdate><title>Repair of surviving hair cells in the damaged mouse utricle</title><author>Kim, Grace S. ; Wang, Tian ; Sayyid, Zahra N. ; Fuhriman, Jessica ; Jones, Sherri M. ; Cheng, Alan G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-6166a26625d2bcbf04817e4152a331fcaf1cd37837538788b105965fe5f144b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Ablation</topic><topic>Animals</topic><topic>Basic Helix-Loop-Helix Transcription Factors - genetics</topic><topic>Biological Sciences</topic><topic>Cell Survival - genetics</topic><topic>Damage detection</topic><topic>Hair</topic><topic>Hair cells</topic><topic>Hair Cells, Vestibular - physiology</topic><topic>Homeodomain Proteins - genetics</topic><topic>Lamination</topic><topic>Math1 protein</topic><topic>Mechanoreceptors</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Recovery</topic><topic>Regeneration - genetics</topic><topic>Repair</topic><topic>RNA, Untranslated - genetics</topic><topic>Saccule and Utricle - cytology</topic><topic>Saccule and Utricle - injuries</topic><topic>Saccule and Utricle - physiology</topic><topic>Sensory neurons</topic><topic>Survival</topic><topic>Synapses</topic><topic>Transcription Factor Brn-3C - genetics</topic><topic>Utricle</topic><topic>Vestibular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Grace S.</creatorcontrib><creatorcontrib>Wang, Tian</creatorcontrib><creatorcontrib>Sayyid, Zahra N.</creatorcontrib><creatorcontrib>Fuhriman, Jessica</creatorcontrib><creatorcontrib>Jones, Sherri M.</creatorcontrib><creatorcontrib>Cheng, Alan G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Grace S.</au><au>Wang, Tian</au><au>Sayyid, Zahra N.</au><au>Fuhriman, Jessica</au><au>Jones, Sherri M.</au><au>Cheng, Alan G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repair of surviving hair cells in the damaged mouse utricle</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2022-04-12</date><risdate>2022</risdate><volume>119</volume><issue>15</issue><spage>1</spage><epage>12</epage><pages>1-12</pages><issn>0027-8424</issn><issn>1091-6490</issn><eissn>1091-6490</eissn><abstract>Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and contribute to this recovery is unclear. Here, we generated the Pou4f3DTR/+; Atoh1CreERTM/+; Rosa26RtdTomato/+
mouse to fate map HCs prior to ablation. After HC ablation, vestibular evoked potentials were abolished in all animals, with ∼57% later recovering responses. Relative to nonrecovery mice, recovery animals harbored more Atoh1-tdTomato⁺ surviving HCs. In both groups, surviving HCs displayed markers of both type I and type II subtypes and afferent synapses, despite distorted lamination and morphology. Surviving type II HCs remained innervated in both groups, whereas surviving type I HCs first lacked and later regained calyces in the recovery, but not the nonrecovery, group. Finally, surviving HCs initially displayed immature and subsequently mature-appearing bundles in the recovery group. These results demonstrate that surviving HCs are capable of self-repair and may contribute to the recovery of vestibular function.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>35380897</pmid><doi>10.1073/pnas.2116973119</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-8784-2953</orcidid><orcidid>https://orcid.org/0000-0002-4702-8401</orcidid><orcidid>https://orcid.org/0000-0001-6872-7028</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0027-8424 |
ispartof | Proceedings of the National Academy of Sciences - PNAS, 2022-04, Vol.119 (15), p.1-12 |
issn | 0027-8424 1091-6490 1091-6490 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9169652 |
source | MEDLINE; PMC (PubMed Central); Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
subjects | Ablation Animals Basic Helix-Loop-Helix Transcription Factors - genetics Biological Sciences Cell Survival - genetics Damage detection Hair Hair cells Hair Cells, Vestibular - physiology Homeodomain Proteins - genetics Lamination Math1 protein Mechanoreceptors Mice Mice, Mutant Strains Recovery Regeneration - genetics Repair RNA, Untranslated - genetics Saccule and Utricle - cytology Saccule and Utricle - injuries Saccule and Utricle - physiology Sensory neurons Survival Synapses Transcription Factor Brn-3C - genetics Utricle Vestibular system |
title | Repair of surviving hair cells in the damaged mouse utricle |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T17%3A06%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Repair%20of%20surviving%20hair%20cells%20in%20the%20damaged%20mouse%20utricle&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Kim,%20Grace%20S.&rft.date=2022-04-12&rft.volume=119&rft.issue=15&rft.spage=1&rft.epage=12&rft.pages=1-12&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.2116973119&rft_dat=%3Cjstor_pubme%3E27151826%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2653335777&rft_id=info:pmid/35380897&rft_jstor_id=27151826&rfr_iscdi=true |