Repair of surviving hair cells in the damaged mouse utricle

Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2022-04, Vol.119 (15), p.1-12
Hauptverfasser: Kim, Grace S., Wang, Tian, Sayyid, Zahra N., Fuhriman, Jessica, Jones, Sherri M., Cheng, Alan G.
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Kim, Grace S.
Wang, Tian
Sayyid, Zahra N.
Fuhriman, Jessica
Jones, Sherri M.
Cheng, Alan G.
description Sensory hair cells (HCs) in the utricle are mechanoreceptors required to detect linear acceleration. After damage, the mammalian utricle partially restores the HC population and organ function, although regenerated HCs are primarily type II and immature. Whether native, surviving HCs can repair and contribute to this recovery is unclear. Here, we generated the Pou4f3DTR/+; Atoh1CreERTM/+; Rosa26RtdTomato/+ mouse to fate map HCs prior to ablation. After HC ablation, vestibular evoked potentials were abolished in all animals, with ∼57% later recovering responses. Relative to nonrecovery mice, recovery animals harbored more Atoh1-tdTomato⁺ surviving HCs. In both groups, surviving HCs displayed markers of both type I and type II subtypes and afferent synapses, despite distorted lamination and morphology. Surviving type II HCs remained innervated in both groups, whereas surviving type I HCs first lacked and later regained calyces in the recovery, but not the nonrecovery, group. Finally, surviving HCs initially displayed immature and subsequently mature-appearing bundles in the recovery group. These results demonstrate that surviving HCs are capable of self-repair and may contribute to the recovery of vestibular function.
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subjects Ablation
Animals
Basic Helix-Loop-Helix Transcription Factors - genetics
Biological Sciences
Cell Survival - genetics
Damage detection
Hair
Hair cells
Hair Cells, Vestibular - physiology
Homeodomain Proteins - genetics
Lamination
Math1 protein
Mechanoreceptors
Mice
Mice, Mutant Strains
Recovery
Regeneration - genetics
Repair
RNA, Untranslated - genetics
Saccule and Utricle - cytology
Saccule and Utricle - injuries
Saccule and Utricle - physiology
Sensory neurons
Survival
Synapses
Transcription Factor Brn-3C - genetics
Utricle
Vestibular system
title Repair of surviving hair cells in the damaged mouse utricle
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