Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source
Lactococcus lactis produced more exopolysaccharide (EPS) on glucose than on fructose as the sugar substrate, although the transcription level of the eps gene cluster was independent of the sugar source. A major difference between cells grown on the two substrates was the capacity to produce sugar nu...
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| Veröffentlicht in: | Applied and environmental microbiology 1999-11, Vol.65 (11), p.5003-5008 |
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| creator | LOOIJESTEIJN, P. J BOELS, I. C KLEEREBEZEM, M HUGENHOLTZ, J |
| description | Lactococcus lactis produced more exopolysaccharide (EPS) on glucose than on fructose as the sugar substrate, although the transcription level of the eps gene cluster was independent of the sugar source. A major difference between cells grown on the two substrates was the capacity to produce sugar nucleotides, the EPS precursors. However, the activities of the enzymes required for the synthesis of nucleotide sugars were not changed upon growth on different sugars. The activity of fructosebisphosphatase (FBPase) was by far the lowest of the enzymes involved in precursor formation under all conditions. FBPase catalyzes the conversion of fructose-1, 6-diphosphate into fructose-6-phosphate, which is an essential step in the biosynthesis of sugar nucleotides from fructose but not from glucose. By overexpression of the fbp gene, which resulted in increased EPS synthesis on fructose, it was proven that the low activity of FBPase is indeed limiting not only for EPS production but also for growth on fructose as a sugar source. |
| doi_str_mv | 10.1128/aem.65.11.5003-5008.1999 |
| format | Article |
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J ; BOELS, I. C ; KLEEREBEZEM, M ; HUGENHOLTZ, J</creator><creatorcontrib>LOOIJESTEIJN, P. J ; BOELS, I. C ; KLEEREBEZEM, M ; HUGENHOLTZ, J</creatorcontrib><description>Lactococcus lactis produced more exopolysaccharide (EPS) on glucose than on fructose as the sugar substrate, although the transcription level of the eps gene cluster was independent of the sugar source. A major difference between cells grown on the two substrates was the capacity to produce sugar nucleotides, the EPS precursors. However, the activities of the enzymes required for the synthesis of nucleotide sugars were not changed upon growth on different sugars. The activity of fructosebisphosphatase (FBPase) was by far the lowest of the enzymes involved in precursor formation under all conditions. FBPase catalyzes the conversion of fructose-1, 6-diphosphate into fructose-6-phosphate, which is an essential step in the biosynthesis of sugar nucleotides from fructose but not from glucose. By overexpression of the fbp gene, which resulted in increased EPS synthesis on fructose, it was proven that the low activity of FBPase is indeed limiting not only for EPS production but also for growth on fructose as a sugar source.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.65.11.5003-5008.1999</identifier><identifier>PMID: 10543815</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Cell Wall - chemistry ; Cell Wall - metabolism ; Fructose - metabolism ; Fructose-Bisphosphatase - genetics ; Fructose-Bisphosphatase - metabolism ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Lactococcus lactis - growth & development ; Lactococcus lactis - metabolism ; Lactococcus lactis cremoris ; Miscellaneous ; Mission oriented research ; Models, Chemical ; Nucleoside Diphosphate Sugars - metabolism ; Physiology and Biotechnology ; Polysaccharides, Bacterial - biosynthesis</subject><ispartof>Applied and environmental microbiology, 1999-11, Vol.65 (11), p.5003-5008</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright © 1999, American Society for Microbiology 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-2e360846f7be129bba7026788743e6ca2dc24b77544593d0a5428f7c9527dad43</citedby><cites>FETCH-LOGICAL-c529t-2e360846f7be129bba7026788743e6ca2dc24b77544593d0a5428f7c9527dad43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC91673/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC91673/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,3176,27906,27907,53773,53775</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1185412$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10543815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LOOIJESTEIJN, P. J</creatorcontrib><creatorcontrib>BOELS, I. C</creatorcontrib><creatorcontrib>KLEEREBEZEM, M</creatorcontrib><creatorcontrib>HUGENHOLTZ, J</creatorcontrib><title>Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Lactococcus lactis produced more exopolysaccharide (EPS) on glucose than on fructose as the sugar substrate, although the transcription level of the eps gene cluster was independent of the sugar source. A major difference between cells grown on the two substrates was the capacity to produce sugar nucleotides, the EPS precursors. However, the activities of the enzymes required for the synthesis of nucleotide sugars were not changed upon growth on different sugars. The activity of fructosebisphosphatase (FBPase) was by far the lowest of the enzymes involved in precursor formation under all conditions. FBPase catalyzes the conversion of fructose-1, 6-diphosphate into fructose-6-phosphate, which is an essential step in the biosynthesis of sugar nucleotides from fructose but not from glucose. By overexpression of the fbp gene, which resulted in increased EPS synthesis on fructose, it was proven that the low activity of FBPase is indeed limiting not only for EPS production but also for growth on fructose as a sugar source.</description><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Cell Wall - chemistry</subject><subject>Cell Wall - metabolism</subject><subject>Fructose - metabolism</subject><subject>Fructose-Bisphosphatase - genetics</subject><subject>Fructose-Bisphosphatase - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Lactococcus lactis - growth & development</subject><subject>Lactococcus lactis - metabolism</subject><subject>Lactococcus lactis cremoris</subject><subject>Miscellaneous</subject><subject>Mission oriented research</subject><subject>Models, Chemical</subject><subject>Nucleoside Diphosphate Sugars - metabolism</subject><subject>Physiology and Biotechnology</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS0EokPhLyAvELsEvx8Sm6oqD2kqJARr68ZxZoKSONhJ1fn39TAjKLturo99v2Pdq4MQpqSmlJkPEMZayaJrSQivSjE1tdY-QxtKrKkk5-o52hBibcWYIBfoVc6_CCGCKPMSXVAiBTdUblD7PezWAZY-Tjh2ONzHOQ6HDN7vIfVtwHOK7er_9JsD3oJfoo_erxkPRfcZ57XJc419CmNM5V6oZR_K8w4SznFNPrxGLzoYcnhzPi_Rz083P66_VNtvn79eX20rL5ldKha4IkaoTjeBMts0oAlT2hgteFAeWOuZaLSWQkjLWwJSMNNpbyXTLbSCX6KPp3_ntRlD68O0JBjcnPoR0sFF6N3_nanfu128c5YqzYv9_dme4u815MWNffZhGGAKcc1OWcbUU0CqudREyAKaE-hTzDmF7u8slLhjku7q5tYpWbQ7Jnksxh2TLNa3j3d5ZDxFV4B3ZwCyh6FLMPk-_-OokYIy_gCGH6md</recordid><startdate>19991101</startdate><enddate>19991101</enddate><creator>LOOIJESTEIJN, P. 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C ; KLEEREBEZEM, M ; HUGENHOLTZ, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-2e360846f7be129bba7026788743e6ca2dc24b77544593d0a5428f7c9527dad43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>Cell Wall - chemistry</topic><topic>Cell Wall - metabolism</topic><topic>Fructose - metabolism</topic><topic>Fructose-Bisphosphatase - genetics</topic><topic>Fructose-Bisphosphatase - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Lactococcus lactis - growth & development</topic><topic>Lactococcus lactis - metabolism</topic><topic>Lactococcus lactis cremoris</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>Models, Chemical</topic><topic>Nucleoside Diphosphate Sugars - metabolism</topic><topic>Physiology and Biotechnology</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LOOIJESTEIJN, P. J</creatorcontrib><creatorcontrib>BOELS, I. 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C</au><au>KLEEREBEZEM, M</au><au>HUGENHOLTZ, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1999-11-01</date><risdate>1999</risdate><volume>65</volume><issue>11</issue><spage>5003</spage><epage>5008</epage><pages>5003-5008</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Lactococcus lactis produced more exopolysaccharide (EPS) on glucose than on fructose as the sugar substrate, although the transcription level of the eps gene cluster was independent of the sugar source. A major difference between cells grown on the two substrates was the capacity to produce sugar nucleotides, the EPS precursors. However, the activities of the enzymes required for the synthesis of nucleotide sugars were not changed upon growth on different sugars. The activity of fructosebisphosphatase (FBPase) was by far the lowest of the enzymes involved in precursor formation under all conditions. FBPase catalyzes the conversion of fructose-1, 6-diphosphate into fructose-6-phosphate, which is an essential step in the biosynthesis of sugar nucleotides from fructose but not from glucose. By overexpression of the fbp gene, which resulted in increased EPS synthesis on fructose, it was proven that the low activity of FBPase is indeed limiting not only for EPS production but also for growth on fructose as a sugar source.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>10543815</pmid><doi>10.1128/aem.65.11.5003-5008.1999</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Cell Wall - chemistry Cell Wall - metabolism Fructose - metabolism Fructose-Bisphosphatase - genetics Fructose-Bisphosphatase - metabolism Fundamental and applied biological sciences. Psychology Glucose - metabolism Lactococcus lactis - growth & development Lactococcus lactis - metabolism Lactococcus lactis cremoris Miscellaneous Mission oriented research Models, Chemical Nucleoside Diphosphate Sugars - metabolism Physiology and Biotechnology Polysaccharides, Bacterial - biosynthesis |
| title | Regulation of exopolysaccharide production by Lactococcus lactis subsp. cremoris by the sugar source |
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