Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption
Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of...
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| Veröffentlicht in: | Epidemiology and infection 2017-11, Vol.145 (15), p.3125-3130 |
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| creator | OLAYA-GALÁN, N. N. CORREDOR-FIGUEROA, A. P. GUZMÁN-GARZÓN, T. C. RÍOS-HERNANDEZ, K. S. SALAS-CÁRDENAS, S. P. PATARROYO, M. A. GUTIERREZ, M. F. |
| description | Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption. |
| doi_str_mv | 10.1017/S0950268817002229 |
| format | Article |
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N. ; CORREDOR-FIGUEROA, A. P. ; GUZMÁN-GARZÓN, T. C. ; RÍOS-HERNANDEZ, K. S. ; SALAS-CÁRDENAS, S. P. ; PATARROYO, M. A. ; GUTIERREZ, M. F.</creator><creatorcontrib>OLAYA-GALÁN, N. N. ; CORREDOR-FIGUEROA, A. P. ; GUZMÁN-GARZÓN, T. C. ; RÍOS-HERNANDEZ, K. S. ; SALAS-CÁRDENAS, S. P. ; PATARROYO, M. A. ; GUTIERREZ, M. F.</creatorcontrib><description>Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.</description><identifier>ISSN: 0950-2688</identifier><identifier>EISSN: 1469-4409</identifier><identifier>DOI: 10.1017/S0950268817002229</identifier><identifier>PMID: 28956522</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Beef ; Cattle ; Deoxyribonucleic acid ; Diagnostic systems ; DNA ; DNA, Viral - genetics ; Enzootic Bovine Leukosis - transmission ; Food consumption ; Food production ; Gag protein ; Humans ; Leukemia ; Leukemia Virus, Bovine - genetics ; Meat ; Meat - virology ; Meat products ; Milk ; Milk - virology ; Multiplex Polymerase Chain Reaction ; Original Papers ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Viral infections ; Viruses ; Zoonoses ; Zoonoses/Animal infections</subject><ispartof>Epidemiology and infection, 2017-11, Vol.145 (15), p.3125-3130</ispartof><rights>Copyright © Cambridge University Press 2017</rights><rights>Cambridge University Press 2017</rights><rights>Copyright © Cambridge University Press 2017 This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Cambridge University Press 2017 2017 Cambridge University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-efc24a0004367e2d24f86c25e54fe372e42b2eb54ad84b77aed4fc791f8ab3c03</citedby><cites>FETCH-LOGICAL-c493t-efc24a0004367e2d24f86c25e54fe372e42b2eb54ad84b77aed4fc791f8ab3c03</cites><orcidid>0000-0002-4282-4951 ; 0000-0002-4751-2500</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26521415$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26521415$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28956522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OLAYA-GALÁN, N. N.</creatorcontrib><creatorcontrib>CORREDOR-FIGUEROA, A. P.</creatorcontrib><creatorcontrib>GUZMÁN-GARZÓN, T. C.</creatorcontrib><creatorcontrib>RÍOS-HERNANDEZ, K. S.</creatorcontrib><creatorcontrib>SALAS-CÁRDENAS, S. P.</creatorcontrib><creatorcontrib>PATARROYO, M. A.</creatorcontrib><creatorcontrib>GUTIERREZ, M. F.</creatorcontrib><title>Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption</title><title>Epidemiology and infection</title><addtitle>Epidemiol. Infect</addtitle><description>Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.</description><subject>Animals</subject><subject>Beef</subject><subject>Cattle</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnostic systems</subject><subject>DNA</subject><subject>DNA, Viral - genetics</subject><subject>Enzootic Bovine Leukosis - transmission</subject><subject>Food consumption</subject><subject>Food production</subject><subject>Gag protein</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia Virus, Bovine - genetics</subject><subject>Meat</subject><subject>Meat - virology</subject><subject>Meat products</subject><subject>Milk</subject><subject>Milk - virology</subject><subject>Multiplex Polymerase Chain Reaction</subject><subject>Original Papers</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Viral infections</subject><subject>Viruses</subject><subject>Zoonoses</subject><subject>Zoonoses/Animal infections</subject><issn>0950-2688</issn><issn>1469-4409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>IKXGN</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kc2OFCEUhYnROO3oA7jQkLhxUwoUFLAxGcffONGFuiYUdZmmpwpaqGrj20un23HUuIHF-e695-Qg9JCSZ5RQ-fwz0YKwTikqCWGM6VtoRXmnG86Jvo1We7nZ6yfoXikbQohmSt5FJ0xp0QnGVujDy7QLEfAIy5WFKVi8C3kp-NXHMxwi9hnKGk9hvMI2Djjb77gH8NinjNfLZCN2KZZl2s4hxfvojrdjgQfH_xR9ffP6y_m75uLT2_fnZxeN47qdG_COcVvN8LaTwAbGveocEyC4h1Yy4Kxn0AtuB8V7KS0M3DupqVe2bx1pT9GLw97t0k8wOIhztqPZ5jDZ_MMkG8yfSgxrc5l2RlOupBB1wdPjgpy-LVBmM4XiYBxthLQUQzXn1VyreEWf_IVu0pJjjVepjov6MFopeqBcTqVk8NdmKDH7qsw_VdWZxzdTXE_86qYCjw7Apswp_9arSDndp2iPR-3U5zBcwg1v_z37E1Ujp3s</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>OLAYA-GALÁN, N. 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N.</au><au>CORREDOR-FIGUEROA, A. P.</au><au>GUZMÁN-GARZÓN, T. C.</au><au>RÍOS-HERNANDEZ, K. S.</au><au>SALAS-CÁRDENAS, S. P.</au><au>PATARROYO, M. A.</au><au>GUTIERREZ, M. F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption</atitle><jtitle>Epidemiology and infection</jtitle><addtitle>Epidemiol. Infect</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>145</volume><issue>15</issue><spage>3125</spage><epage>3130</epage><pages>3125-3130</pages><issn>0950-2688</issn><eissn>1469-4409</eissn><abstract>Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>28956522</pmid><doi>10.1017/S0950268817002229</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-4282-4951</orcidid><orcidid>https://orcid.org/0000-0002-4751-2500</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Beef Cattle Deoxyribonucleic acid Diagnostic systems DNA DNA, Viral - genetics Enzootic Bovine Leukosis - transmission Food consumption Food production Gag protein Humans Leukemia Leukemia Virus, Bovine - genetics Meat Meat - virology Meat products Milk Milk - virology Multiplex Polymerase Chain Reaction Original Papers Polymerase Chain Reaction Sequence Analysis, DNA Viral infections Viruses Zoonoses Zoonoses/Animal infections |
| title | Bovine leukaemia virus DNA in fresh milk and raw beef for human consumption |
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