Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines
In cancer cells, cell-surface sialylation is altered, including a change in oligo/polysialic acid (oligo/polySia) structures. Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and...
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| Veröffentlicht in: | International journal of molecular sciences 2022-05, Vol.23 (10), p.5569 |
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| creator | Hane, Masaya Kitajima, Ken Sato, Chihiro |
| description | In cancer cells, cell-surface sialylation is altered, including a change in oligo/polysialic acid (oligo/polySia) structures. Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and treatment of the disease, a precise understanding of the oligo/polySia structures in cancer cells is necessary. In this study, flow cytometric analysis and gene expression datasets were obtained from sixteen different cancer cell lines. These datasets demonstrated the ability to predict glycan structures and their sialylation status. Our results also revealed that sialylation patterns are unique to each cancer cell line. Thus, we can suggest promising combinations of antibody and cancer cell for glycan prediction. However, the precise prediction of minor glycans need to be further explored. |
| doi_str_mv | 10.3390/ijms23105569 |
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Since they are unique and rarely expressed in normal cells, oligo/polySia structures may serve as promising novel biomarkers and targets for therapies. For the diagnosis and treatment of the disease, a precise understanding of the oligo/polySia structures in cancer cells is necessary. In this study, flow cytometric analysis and gene expression datasets were obtained from sixteen different cancer cell lines. These datasets demonstrated the ability to predict glycan structures and their sialylation status. Our results also revealed that sialylation patterns are unique to each cancer cell line. Thus, we can suggest promising combinations of antibody and cancer cell for glycan prediction. 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| subjects | Acids Antibodies Biomarkers Biosynthesis Cancer Cell surface Datasets Enzymes Flow cytometry Gene expression Gene flow Glycan Glycoproteins Hypoxia Neuroblastoma Polysaccharides Polysialic acid Tumor cell lines |
| title | Comprehensive Analysis of Oligo/Polysialylglycoconjugates in Cancer Cell Lines |
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