DIPA-CRISPR is a simple and accessible method for insect gene editing

Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome thes...

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Veröffentlicht in:	Cell reports methods 2022-05, Vol.2 (5), p.100215-100215, Article 100215
Hauptverfasser:	Shirai, Yu, Piulachs, Maria-Dolors, Belles, Xavier, Daimon, Takaaki
Format:	Artikel
Sprache:	eng
Schlagworte:	adults beetles cockroaches CRISPR-associated endonuclease 9 CRISPR-Cas9 DIPA-CRISPR gene editing genes genome editing hemocoel insects oocytes reproduction ribonucleoproteins Tribolium castaneum
Online-Zugang:	Volltext
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