A new biocatalyst for production of optically pure aryl epoxides by styrene monooxygenase from Pseudomonas fluorescens ST

We developed a biocatalyst by cloning the styrene monooxygenase genes (styA and styB) from Pseudomonas fluorescens ST responsible for the oxidation of styrene to its corresponding epoxide. Recombinant Escherichia coli was able to oxidize different aryl vinyl and aryl ethenyl compounds to their corre...

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Veröffentlicht in:	Applied and environmental microbiology 1999-06, Vol.65 (6), p.2794-2797
Hauptverfasser:	DI GENNARO, P, COLMEGNA, A, GALLI, E, SELLO, G, PELIZZONI, F, BESTETTI, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Catalysis Enzymes Epoxy Compounds - chemistry Epoxy Compounds - metabolism Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Magnetic Resonance Spectroscopy Methods. Procedures. Technologies Oxygenases - genetics Oxygenases - metabolism Physiology and Biotechnology Pseudomonas fluorescens Pseudomonas fluorescens - enzymology Pseudomonas fluorescens - genetics Pseudomonas fluorescens - metabolism Recombinant Proteins - metabolism
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