KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds
Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban pon...
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| Veröffentlicht in: | International journal of environmental research and public health 2022-05, Vol.19 (10), p.5848 |
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| creator | Teixeira, Pedro Pinto, Nuno Henriques, Isabel Tacão, Marta |
| description | Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant
(CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with
(
= 1),
(
= 11),
(
= 8), and
(
= 3). Carbapenemase-encoding genes were detected in 21 isolates:
(
= 20),
(
= 6), and
(
= 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns.
F6,
N9, and
N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that
was located on Tn
b (
F6 and
N10) or Tn
d (
N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition,
was in a class 3 integron, either chromosomal (
N9) or plasmidic (
N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE. |
| doi_str_mv | 10.3390/ijerph19105848 |
| format | Article |
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(CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with
(
= 1),
(
= 11),
(
= 8), and
(
= 3). Carbapenemase-encoding genes were detected in 21 isolates:
(
= 20),
(
= 6), and
(
= 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns.
F6,
N9, and
N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that
was located on Tn
b (
F6 and
N10) or Tn
d (
N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition,
was in a class 3 integron, either chromosomal (
N9) or plasmidic (
N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph19105848</identifier><identifier>PMID: 35627386</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Antibiotics ; Antimicrobial agents ; Bacteria ; Carbapenemase ; Carbapenems ; Citrobacter ; Conjugation ; Dispersal ; Drug resistance ; Enterobacter ; Enterobacterales ; Genes ; Genomes ; Klebsiella ; Multidrug resistant organisms ; Nucleotide sequence ; Pathogens ; Plasmids ; Ponds ; Raoultella ; rRNA 16S ; Sequence analysis ; Surface water</subject><ispartof>International journal of environmental research and public health, 2022-05, Vol.19 (10), p.5848</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3338-afd1bc3174b09910d694c5f4148e4c5be8c673e10a4d76e95c4456ffc9daaca13</citedby><cites>FETCH-LOGICAL-c3338-afd1bc3174b09910d694c5f4148e4c5be8c673e10a4d76e95c4456ffc9daaca13</cites><orcidid>0000-0001-7965-4928 ; 0000-0002-3133-8843</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141432/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9141432/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35627386$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teixeira, Pedro</creatorcontrib><creatorcontrib>Pinto, Nuno</creatorcontrib><creatorcontrib>Henriques, Isabel</creatorcontrib><creatorcontrib>Tacão, Marta</creatorcontrib><title>KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant
(CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with
(
= 1),
(
= 11),
(
= 8), and
(
= 3). Carbapenemase-encoding genes were detected in 21 isolates:
(
= 20),
(
= 6), and
(
= 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns.
F6,
N9, and
N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that
was located on Tn
b (
F6 and
N10) or Tn
d (
N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition,
was in a class 3 integron, either chromosomal (
N9) or plasmidic (
N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE.</description><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Carbapenemase</subject><subject>Carbapenems</subject><subject>Citrobacter</subject><subject>Conjugation</subject><subject>Dispersal</subject><subject>Drug resistance</subject><subject>Enterobacter</subject><subject>Enterobacterales</subject><subject>Genes</subject><subject>Genomes</subject><subject>Klebsiella</subject><subject>Multidrug resistant organisms</subject><subject>Nucleotide sequence</subject><subject>Pathogens</subject><subject>Plasmids</subject><subject>Ponds</subject><subject>Raoultella</subject><subject>rRNA 16S</subject><subject>Sequence analysis</subject><subject>Surface water</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkUtLAzEUhYMovrcuZcCNC6O5TSaTbAQpVYuvgo9tyCQZnTJNatIR_Pem-EBdnQv57sm5HIT2gBxTKslJO3Vx_gISSCmYWEGbwDnBjBNY_TVvoK2UpoRQwbhcRxu05IOKCr6Jbq8mQ0zxUXExusdlVu1t8TS-wYAnMdjetP65GPmFi6HWJovuXCrGKXR64WzRxDArHmOtfTEJ3qYdtNboLrndL91Gj-ejh-Elvr67GA_PrrGhlAqsGwu1oVCxmsic3XLJTNkwYMLloXbC8Io6IJrZijtZGsZK3jRGWq2NBrqNTj995309c9Y4v8jJ1Dy2Mx3fVdCt-vvi2xf1HN6UhPwJHWSDwy-DGF57lxZq1ibjuk57F_qkBryCgSAcREYP_qHT0Eefz1tSBEQFcml4_EmZGFKKrvkJA0Qtq1J_q8oL-79P-MG_u6EfIHeOCg</recordid><startdate>20220511</startdate><enddate>20220511</enddate><creator>Teixeira, Pedro</creator><creator>Pinto, Nuno</creator><creator>Henriques, Isabel</creator><creator>Tacão, Marta</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7965-4928</orcidid><orcidid>https://orcid.org/0000-0002-3133-8843</orcidid></search><sort><creationdate>20220511</creationdate><title>KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds</title><author>Teixeira, Pedro ; Pinto, Nuno ; Henriques, Isabel ; Tacão, Marta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3338-afd1bc3174b09910d694c5f4148e4c5be8c673e10a4d76e95c4456ffc9daaca13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Carbapenemase</topic><topic>Carbapenems</topic><topic>Citrobacter</topic><topic>Conjugation</topic><topic>Dispersal</topic><topic>Drug resistance</topic><topic>Enterobacter</topic><topic>Enterobacterales</topic><topic>Genes</topic><topic>Genomes</topic><topic>Klebsiella</topic><topic>Multidrug resistant organisms</topic><topic>Nucleotide sequence</topic><topic>Pathogens</topic><topic>Plasmids</topic><topic>Ponds</topic><topic>Raoultella</topic><topic>rRNA 16S</topic><topic>Sequence analysis</topic><topic>Surface water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teixeira, Pedro</creatorcontrib><creatorcontrib>Pinto, Nuno</creatorcontrib><creatorcontrib>Henriques, Isabel</creatorcontrib><creatorcontrib>Tacão, Marta</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of environmental research and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teixeira, Pedro</au><au>Pinto, Nuno</au><au>Henriques, Isabel</au><au>Tacão, Marta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds</atitle><jtitle>International journal of environmental research and public health</jtitle><addtitle>Int J Environ Res Public Health</addtitle><date>2022-05-11</date><risdate>2022</risdate><volume>19</volume><issue>10</issue><spage>5848</spage><pages>5848-</pages><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant
(CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with
(
= 1),
(
= 11),
(
= 8), and
(
= 3). Carbapenemase-encoding genes were detected in 21 isolates:
(
= 20),
(
= 6), and
(
= 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns.
F6,
N9, and
N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that
was located on Tn
b (
F6 and
N10) or Tn
d (
N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition,
was in a class 3 integron, either chromosomal (
N9) or plasmidic (
N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35627386</pmid><doi>10.3390/ijerph19105848</doi><orcidid>https://orcid.org/0000-0001-7965-4928</orcidid><orcidid>https://orcid.org/0000-0002-3133-8843</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of environmental research and public health, 2022-05, Vol.19 (10), p.5848 |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; PubMed Central Open Access |
| subjects | Antibiotics Antimicrobial agents Bacteria Carbapenemase Carbapenems Citrobacter Conjugation Dispersal Drug resistance Enterobacter Enterobacterales Genes Genomes Klebsiella Multidrug resistant organisms Nucleotide sequence Pathogens Plasmids Ponds Raoultella rRNA 16S Sequence analysis Surface water |
| title | KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds |
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