Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana
Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extens...
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| Veröffentlicht in: | International journal of molecular sciences 2022-05, Vol.23 (10), p.5375 |
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| creator | Marzol, Eliana Borassi, Cecilia Carignani Sardoy, Mariana Ranocha, Philippe Aptekmann, Ariel A Bringas, Mauro Pennington, Janice Paez-Valencia, Julio Martínez Pacheco, Javier Rodríguez-Garcia, Diana R Rondón Guerrero, Yossmayer Del Carmen Peralta, Juan Manuel Fleming, Margaret Mishler-Elmore, John W Mangano, Silvina Blanco-Herrera, Francisca Bedinger, Patricia A Dunand, Christophe Capece, Luciana Nadra, Alejandro D Held, Michael Otegui, Marisa S Estevez, José M |
| description | Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast and oscillatory growth is mediated by continuous remodeling of the cell wall. Root hair cell walls contain polysaccharides and hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) are secreted into the apoplastic space and are thought to trigger either cell wall loosening or polymerization of cell wall components, such as Tyr-mediated assembly of EXT networks (EXT-PRXs). The precise role of these EXT-PRXs is unknown. Using genetic, biochemical, and modeling approaches, we identified and characterized three root-hair-specific putative EXT-PRXs, PRX01, PRX44, and PRX73.
triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in
and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells. |
| doi_str_mv | 10.3390/ijms23105375 |
| format | Article |
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triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in
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triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in
and PRX44-overexpressing background plants. In this study, we propose that PRX01, PRX44, and PRX73 control EXT-mediated cell wall properties during polar expansion of root hair cells.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - chemistry</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Cell Wall</subject><subject>Cell walls</subject><subject>Fluorescence</subject><subject>Glycoproteins</subject><subject>Hair cells</subject><subject>Homeostasis</subject><subject>Hydroxyproline</subject><subject>Mutation</subject><subject>Nutrients</subject><subject>Peroxidase</subject><subject>Peroxidases - genetics</subject><subject>Plant Roots - genetics</subject><subject>Polysaccharides</subject><subject>Soil conditions</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtLAzEUhYMo1tfOtQTcuGg1j5lkshFK8VEQFFFwZbiduWNTppOaTH38e6e0lurqHrgfh3M4hBxzdi6lYRduMo1CcpZKnW6RPZ4I0WNM6e0N3SH7MU4YE1KkZpd0ZKpExjOzR14HFcRIh8MhfcDgv1wBESN9eHxhvLs4SdKlUBcLqSUd-LoJvqKP3jf0FlygN8F_NmPqatoPMHKFn0UXaTOGykENh2SnhCri0eoekOfrq6fBbe_u_mY46N_1cil02lOoBI4KLDFTidEsKRGlUCxXZalRlgg6RQEsy2VuMkiz0hgQcgSgFGgAeUAul76z-WiKRY5tTKjsLLgphG_rwdm_n9qN7Zv_sIYnXArRGpytDIJ_n2Ns7NTFHKsKavTzaIXSXOhMa9aip__QiZ-Huq23oBg3SaZ0S3WXVB58jAHLdRjO7GI4uzlci59sFljDv0vJH2Pdks8</recordid><startdate>20220511</startdate><enddate>20220511</enddate><creator>Marzol, Eliana</creator><creator>Borassi, Cecilia</creator><creator>Carignani Sardoy, Mariana</creator><creator>Ranocha, Philippe</creator><creator>Aptekmann, Ariel A</creator><creator>Bringas, Mauro</creator><creator>Pennington, Janice</creator><creator>Paez-Valencia, Julio</creator><creator>Martínez Pacheco, Javier</creator><creator>Rodríguez-Garcia, Diana R</creator><creator>Rondón Guerrero, Yossmayer Del Carmen</creator><creator>Peralta, Juan Manuel</creator><creator>Fleming, Margaret</creator><creator>Mishler-Elmore, John W</creator><creator>Mangano, Silvina</creator><creator>Blanco-Herrera, Francisca</creator><creator>Bedinger, Patricia A</creator><creator>Dunand, Christophe</creator><creator>Capece, Luciana</creator><creator>Nadra, Alejandro D</creator><creator>Held, Michael</creator><creator>Otegui, Marisa S</creator><creator>Estevez, José M</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4699-6950</orcidid><orcidid>https://orcid.org/0000-0003-3220-1221</orcidid><orcidid>https://orcid.org/0000-0002-7860-3245</orcidid><orcidid>https://orcid.org/0000-0001-6332-7738</orcidid><orcidid>https://orcid.org/0000-0001-8822-4372</orcidid><orcidid>https://orcid.org/0000-0002-2040-3689</orcidid><orcidid>https://orcid.org/0000-0001-6365-363X</orcidid><orcidid>https://orcid.org/0000-0002-7497-9907</orcidid><orcidid>https://orcid.org/0000-0001-6280-6729</orcidid><orcidid>https://orcid.org/0000-0003-1637-4042</orcidid><orcidid>https://orcid.org/0000-0003-2326-4933</orcidid></search><sort><creationdate>20220511</creationdate><title>Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana</title><author>Marzol, Eliana ; Borassi, Cecilia ; Carignani Sardoy, Mariana ; Ranocha, Philippe ; Aptekmann, Ariel A ; Bringas, Mauro ; Pennington, Janice ; Paez-Valencia, Julio ; Martínez Pacheco, Javier ; Rodríguez-Garcia, Diana R ; Rondón Guerrero, Yossmayer Del Carmen ; Peralta, Juan Manuel ; Fleming, Margaret ; Mishler-Elmore, John W ; Mangano, Silvina ; Blanco-Herrera, Francisca ; Bedinger, Patricia A ; Dunand, Christophe ; Capece, Luciana ; Nadra, Alejandro D ; Held, Michael ; Otegui, Marisa S ; Estevez, José M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3275-6e62ebdefe8649704fee3260c6ff7e3fea75e2a08c3c98a58f99a23baa66a7aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Arabidopsis - 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triple mutation and PRX44 and PRX73 overexpression had opposite effects on root hair growth, peroxidase activity, and ROS production, with a clear impact on cell wall thickness. We use an EXT fluorescent reporter with contrasting levels of cell wall insolubilization in
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| subjects | Arabidopsis - genetics Arabidopsis Proteins - chemistry Arabidopsis Proteins - genetics Cell Wall Cell walls Fluorescence Glycoproteins Hair cells Homeostasis Hydroxyproline Mutation Nutrients Peroxidase Peroxidases - genetics Plant Roots - genetics Polysaccharides Soil conditions |
| title | Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana |
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