Single-cell transcriptomics reveals age-resistant maintenance of cell identities, stem cell compartments and differentiation trajectories in long-lived naked mole-rats skin
Naked mole-rats (NMR) are subterranean rodents characterized by an unusual longevity coupled with an unexplained resistance to aging. In the present study, we performed extensive analysis and single-cell RNA-sequencing comparing young and older animals. At variance with other species, NMR exhibited...
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| Veröffentlicht in: | Aging (Albany, NY.) NY.), 2022-05, Vol.14 (9), p.3728-3756 |
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| creator | Savina, Aleksandra Jaffredo, Thierry Saldmann, Frederic Faulkes, Chris G Moguelet, Philippe Leroy, Christine Marmol, Delphine Del Codogno, Patrice Foucher, Lucy Zalc, Antoine Viltard, Mélanie Friedlander, Gérard Aractingi, Selim Fontaine, Romain H |
| description | Naked mole-rats (NMR) are subterranean rodents characterized by an unusual longevity coupled with an unexplained resistance to aging. In the present study, we performed extensive
analysis and single-cell RNA-sequencing comparing young and older animals. At variance with other species, NMR exhibited a striking stability of skin compartments and cell types, which remained stable over time without aging-associated changes. Remarkably, the number of stem cells was constant throughout aging. We found three classical cellular states defining a unique keratinocyte differentiation trajectory that were not altered after pseudo-temporal reconstruction. Epidermal gene expression did not change with aging either. Langerhans cell clusters were conserved, and only a higher basal stem cell expression of
was found in aged animals. In accordance, NMR skin healing closure was similar in young and older animals. Altogether, these results indicate that NMR skin is characterized by peculiar genetic and cellular features, different from those previously demonstrated for mice and humans. The remarkable stability of the aging NMR skin transcriptome likely reflects unaltered homeostasis and resilience. |
| doi_str_mv | 10.18632/aging.204054 |
| format | Article |
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analysis and single-cell RNA-sequencing comparing young and older animals. At variance with other species, NMR exhibited a striking stability of skin compartments and cell types, which remained stable over time without aging-associated changes. Remarkably, the number of stem cells was constant throughout aging. We found three classical cellular states defining a unique keratinocyte differentiation trajectory that were not altered after pseudo-temporal reconstruction. Epidermal gene expression did not change with aging either. Langerhans cell clusters were conserved, and only a higher basal stem cell expression of
was found in aged animals. In accordance, NMR skin healing closure was similar in young and older animals. Altogether, these results indicate that NMR skin is characterized by peculiar genetic and cellular features, different from those previously demonstrated for mice and humans. The remarkable stability of the aging NMR skin transcriptome likely reflects unaltered homeostasis and resilience.</description><identifier>ISSN: 1945-4589</identifier><identifier>EISSN: 1945-4589</identifier><identifier>DOI: 10.18632/aging.204054</identifier><identifier>PMID: 35507806</identifier><language>eng</language><publisher>United States: Impact Journals</publisher><subject>Aging - genetics ; Animals ; Life Sciences ; Longevity - genetics ; Mice ; Mole Rats - genetics ; Research Paper ; Stem Cells ; Transcriptome</subject><ispartof>Aging (Albany, NY.), 2022-05, Vol.14 (9), p.3728-3756</ispartof><rights>Attribution</rights><rights>Copyright: © 2022 Savina et al.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-77b12698db606a29284ec9e2b74819b51b4062dd176f56b2ba8a4e97f4cd73873</citedby><cites>FETCH-LOGICAL-c421t-77b12698db606a29284ec9e2b74819b51b4062dd176f56b2ba8a4e97f4cd73873</cites><orcidid>0000-0002-8724-3261</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134947/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9134947/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35507806$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://cnrs.hal.science/hal-03977899$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Savina, Aleksandra</creatorcontrib><creatorcontrib>Jaffredo, Thierry</creatorcontrib><creatorcontrib>Saldmann, Frederic</creatorcontrib><creatorcontrib>Faulkes, Chris G</creatorcontrib><creatorcontrib>Moguelet, Philippe</creatorcontrib><creatorcontrib>Leroy, Christine</creatorcontrib><creatorcontrib>Marmol, Delphine Del</creatorcontrib><creatorcontrib>Codogno, Patrice</creatorcontrib><creatorcontrib>Foucher, Lucy</creatorcontrib><creatorcontrib>Zalc, Antoine</creatorcontrib><creatorcontrib>Viltard, Mélanie</creatorcontrib><creatorcontrib>Friedlander, Gérard</creatorcontrib><creatorcontrib>Aractingi, Selim</creatorcontrib><creatorcontrib>Fontaine, Romain H</creatorcontrib><title>Single-cell transcriptomics reveals age-resistant maintenance of cell identities, stem cell compartments and differentiation trajectories in long-lived naked mole-rats skin</title><title>Aging (Albany, NY.)</title><addtitle>Aging (Albany NY)</addtitle><description>Naked mole-rats (NMR) are subterranean rodents characterized by an unusual longevity coupled with an unexplained resistance to aging. In the present study, we performed extensive
analysis and single-cell RNA-sequencing comparing young and older animals. At variance with other species, NMR exhibited a striking stability of skin compartments and cell types, which remained stable over time without aging-associated changes. Remarkably, the number of stem cells was constant throughout aging. We found three classical cellular states defining a unique keratinocyte differentiation trajectory that were not altered after pseudo-temporal reconstruction. Epidermal gene expression did not change with aging either. Langerhans cell clusters were conserved, and only a higher basal stem cell expression of
was found in aged animals. In accordance, NMR skin healing closure was similar in young and older animals. Altogether, these results indicate that NMR skin is characterized by peculiar genetic and cellular features, different from those previously demonstrated for mice and humans. The remarkable stability of the aging NMR skin transcriptome likely reflects unaltered homeostasis and resilience.</description><subject>Aging - genetics</subject><subject>Animals</subject><subject>Life Sciences</subject><subject>Longevity - genetics</subject><subject>Mice</subject><subject>Mole Rats - genetics</subject><subject>Research Paper</subject><subject>Stem Cells</subject><subject>Transcriptome</subject><issn>1945-4589</issn><issn>1945-4589</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUsFuFSEUJcbG1urSrWGpidMCw8CwMWkatSYv6aK6JgxzZ0o7A0_gvaT_5EfKvKlNdQPk3nPOPcBB6B0lZ7QVNTs3o_PjGSOcNPwFOqGKNxVvWvXy2fkYvU7pjhDRNFy8Qsd10xDZEnGCft8U9gSVhWnCORqfbHTbHGZnE46wBzMlbEaoIiSXsvEZz8b5DN54CzgM-MB0PfjssoP0CacM81q1Yd6amOfSKyK-x70bBogL1GQX_DLwDmwOsRCx83gKfqwmt4cee3Nf1jkUb9EUerp3_g06GoofePu4n6KfX7_8uLyqNtffvl9ebCrLGc2VlB1lQrV9J4gwTLGWg1XAOslbqrqGdpwI1vdUiqERHetMazgoOXDby7qV9Sn6vOpud90MvS2Go5n0NrrZxAcdjNP_dry71WPYa0Vrrvgi8HEVuP2PdnWx0UuN1ErKVqk9LdgPj8Ni-LWDlPXs0vJ8xkPYJc2EIEQtqgVarVAbQ0oRhidtSvQhDfqQBr2moeDfP7_HE_rv99d_AIvUtkI</recordid><startdate>20220504</startdate><enddate>20220504</enddate><creator>Savina, Aleksandra</creator><creator>Jaffredo, Thierry</creator><creator>Saldmann, Frederic</creator><creator>Faulkes, Chris G</creator><creator>Moguelet, Philippe</creator><creator>Leroy, Christine</creator><creator>Marmol, Delphine Del</creator><creator>Codogno, Patrice</creator><creator>Foucher, Lucy</creator><creator>Zalc, Antoine</creator><creator>Viltard, Mélanie</creator><creator>Friedlander, Gérard</creator><creator>Aractingi, Selim</creator><creator>Fontaine, Romain H</creator><general>Impact Journals</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-8724-3261</orcidid></search><sort><creationdate>20220504</creationdate><title>Single-cell transcriptomics reveals age-resistant maintenance of cell identities, stem cell compartments and differentiation trajectories in long-lived naked mole-rats skin</title><author>Savina, Aleksandra ; Jaffredo, Thierry ; Saldmann, Frederic ; Faulkes, Chris G ; Moguelet, Philippe ; Leroy, Christine ; Marmol, Delphine Del ; Codogno, Patrice ; Foucher, Lucy ; Zalc, Antoine ; Viltard, Mélanie ; Friedlander, Gérard ; Aractingi, Selim ; Fontaine, Romain H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-77b12698db606a29284ec9e2b74819b51b4062dd176f56b2ba8a4e97f4cd73873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aging - genetics</topic><topic>Animals</topic><topic>Life Sciences</topic><topic>Longevity - genetics</topic><topic>Mice</topic><topic>Mole Rats - genetics</topic><topic>Research Paper</topic><topic>Stem Cells</topic><topic>Transcriptome</topic><toplevel>online_resources</toplevel><creatorcontrib>Savina, Aleksandra</creatorcontrib><creatorcontrib>Jaffredo, Thierry</creatorcontrib><creatorcontrib>Saldmann, Frederic</creatorcontrib><creatorcontrib>Faulkes, Chris G</creatorcontrib><creatorcontrib>Moguelet, Philippe</creatorcontrib><creatorcontrib>Leroy, Christine</creatorcontrib><creatorcontrib>Marmol, Delphine Del</creatorcontrib><creatorcontrib>Codogno, Patrice</creatorcontrib><creatorcontrib>Foucher, Lucy</creatorcontrib><creatorcontrib>Zalc, Antoine</creatorcontrib><creatorcontrib>Viltard, Mélanie</creatorcontrib><creatorcontrib>Friedlander, Gérard</creatorcontrib><creatorcontrib>Aractingi, Selim</creatorcontrib><creatorcontrib>Fontaine, Romain H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Aging (Albany, NY.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savina, Aleksandra</au><au>Jaffredo, Thierry</au><au>Saldmann, Frederic</au><au>Faulkes, Chris G</au><au>Moguelet, Philippe</au><au>Leroy, Christine</au><au>Marmol, Delphine Del</au><au>Codogno, Patrice</au><au>Foucher, Lucy</au><au>Zalc, Antoine</au><au>Viltard, Mélanie</au><au>Friedlander, Gérard</au><au>Aractingi, Selim</au><au>Fontaine, Romain H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-cell transcriptomics reveals age-resistant maintenance of cell identities, stem cell compartments and differentiation trajectories in long-lived naked mole-rats skin</atitle><jtitle>Aging (Albany, NY.)</jtitle><addtitle>Aging (Albany NY)</addtitle><date>2022-05-04</date><risdate>2022</risdate><volume>14</volume><issue>9</issue><spage>3728</spage><epage>3756</epage><pages>3728-3756</pages><issn>1945-4589</issn><eissn>1945-4589</eissn><abstract>Naked mole-rats (NMR) are subterranean rodents characterized by an unusual longevity coupled with an unexplained resistance to aging. In the present study, we performed extensive
analysis and single-cell RNA-sequencing comparing young and older animals. At variance with other species, NMR exhibited a striking stability of skin compartments and cell types, which remained stable over time without aging-associated changes. Remarkably, the number of stem cells was constant throughout aging. We found three classical cellular states defining a unique keratinocyte differentiation trajectory that were not altered after pseudo-temporal reconstruction. Epidermal gene expression did not change with aging either. Langerhans cell clusters were conserved, and only a higher basal stem cell expression of
was found in aged animals. In accordance, NMR skin healing closure was similar in young and older animals. Altogether, these results indicate that NMR skin is characterized by peculiar genetic and cellular features, different from those previously demonstrated for mice and humans. The remarkable stability of the aging NMR skin transcriptome likely reflects unaltered homeostasis and resilience.</abstract><cop>United States</cop><pub>Impact Journals</pub><pmid>35507806</pmid><doi>10.18632/aging.204054</doi><tpages>29</tpages><orcidid>https://orcid.org/0000-0002-8724-3261</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Aging - genetics Animals Life Sciences Longevity - genetics Mice Mole Rats - genetics Research Paper Stem Cells Transcriptome |
| title | Single-cell transcriptomics reveals age-resistant maintenance of cell identities, stem cell compartments and differentiation trajectories in long-lived naked mole-rats skin |
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