Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging

Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon...

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Veröffentlicht in:Journal of cerebral blood flow and metabolism 2022-06, Vol.42 (6), p.966-978
Hauptverfasser: Bonney, Stephanie K, Sullivan, Liam T, Cherry, Timothy J, Daneman, Richard, Shih, Andy Y
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container_end_page 978
container_issue 6
container_start_page 966
container_title Journal of cerebral blood flow and metabolism
container_volume 42
creator Bonney, Stephanie K
Sullivan, Liam T
Cherry, Timothy J
Daneman, Richard
Shih, Andy Y
description Perivascular fibroblasts (PVFs) are recognized for their pro-fibrotic role in many central nervous system disorders. Like mural cells, PVFs surround blood vessels and express Pdgfrβ. However, these shared attributes hinder the ability to distinguish PVFs from mural cells. We used in vivo two-photon imaging and transgenic mice with PVF-targeting promoters (Col1a1 or Col1a2) to compare the structure and distribution of PVFs and mural cells in cerebral cortex of healthy, adult mice. We show that PVFs localize to all cortical penetrating arterioles and their offshoots (arteriole-capillary transition zone), as well as the main trunk of only larger ascending venules. However, the capillary zone is devoid of PVF coverage. PVFs display short-range mobility along the vessel wall and exhibit distinct structural features (flattened somata and thin ruffled processes) not seen with smooth muscle cells or pericytes. These findings clarify that PVFs and mural cells are distinct cell types coexisting in a similar perivascular niche.
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subjects Animals
Brain - blood supply
Capillaries - diagnostic imaging
Fibroblasts - metabolism
Mice
Mice, Transgenic
Original
Pericytes - metabolism
title Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging
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