Degron tagging of BleoR and other antibiotic-resistance genes selects for higher expression of linked transgenes and improved exosome engineering

Five antibiotic resistance (AR) genes have been used to select for transgenic eukaryotic cell lines, with the BleoR, PuroR, HygR, NeoR, and BsdR cassettes conferring resistance to zeocin, puromycin, hygromycin, geneticin/G418, and blasticidin, respectively. We recently demonstrated that each AR gene...

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Veröffentlicht in:	The Journal of biological chemistry 2022-05, Vol.298 (5), p.101846, Article 101846
Hauptverfasser:	Tsai, Shang Jui, Ai, Yiwei, Guo, Chenxu, Gould, Stephen J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology antibiotic blasticidin CD63 Cell Line Drug Resistance - genetics Exosomes - genetics extracellular vesicle G418 Humans hygromycin Proteins - metabolism puromycin selectable marker Transgenes transgenic zeocin
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